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Guilian YANG


Chunfeng WANG


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.5 P.348-365


Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells

Author(s):  Guan WANG, Mingyue HAO, Qiong LIU, Yanlong JIANG, Haibin HUANG, Guilian YANG, Chunfeng WANG

Affiliation(s):  College of Veterinary Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun 130118, China; more

Corresponding email(s):   wangchunfeng@jlau.edu.cn, yangguilian@jlau.edu.cn

Key Words:  Oxidative stress, Apoptosis, Human umbilical vein endothelial cell (HUVEC), Hydrogen peroxide (H2O2)

Guan WANG, Mingyue HAO, Qiong LIU, Yanlong JIANG, Haibin HUANG, Guilian YANG, Chunfeng WANG. Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells[J]. Journal of Zhejiang University Science B, 2021, 22(5): 348-365.

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author="Guan WANG, Mingyue HAO, Qiong LIU, Yanlong JIANG, Haibin HUANG, Guilian YANG, Chunfeng WANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells
%A Guan WANG
%A Mingyue HAO
%A Qiong LIU
%A Yanlong JIANG
%A Haibin HUANG
%A Guilian YANG
%A Chunfeng WANG
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 5
%P 348-365
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000441

T1 - Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells
A1 - Guan WANG
A1 - Mingyue HAO
A1 - Qiong LIU
A1 - Yanlong JIANG
A1 - Haibin HUANG
A1 - Guilian YANG
A1 - Chunfeng WANG
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 5
SP - 348
EP - 365
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000441

This study probed the protective effect of recombinant Lactobacillus plantarum against hydrogen peroxide (H2O2)-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). We constructed a new functional L. plantarum (NC8-pSIP409-alr-angiotensin-converting enzyme inhibitory peptide (ACEIP)) with a double-gene-labeled non-resistant screen as an expression vector. A 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric assay was carried out to determine the cell viability of HUVEC cells following pretreatment with NC8-pSIP409-alr-ACEIP. Flow cytometry (FCM) was used to determine the apoptosis rate of HUVEC cells. Cysteinyl aspartate specific proteinase (caspase)-3/8/9 activity was also assayed and western blotting was used to determine protein expression of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), inducible nitric oxide synthase (iNOS), nicotinamide adenine dinucleotide phosphate oxidase 2 (gp91phox), angiotensin II (AngII), and angiotensin-converting enzyme 2 (ACE2), as well as corresponding indicators of oxidative stress, such as reactive oxygen species (ROS), mitochondrial membrane potential (MMP), malondialdehyde (MDA), and superoxide dismutase (SOD). NC8-pSIP409-alr-ACEIP attenuated H2O2-induced cell death, as determined by the MTT assay. NC8-pSIP409-alr-ACEIP reduced apoptosis of HUVEC cells by FCM. In addition, compared to the positive control, the oxidative stress index of the H2O2-induced HUVEC (Hy-HUVEC), which was pretreated by NC8-pSIP409-alr-ACEIP, iNOS, gp91phox, MDA, and ROS, was decreased obviously; SOD expression level was increased; caspase-3 or -9 was decreased, but caspase-8 did not change; Bcl-2/Bax ratio was increased; permeability changes of mitochondria were inhibited; and loss of transmembrane potential was prevented. Expression of the hypertension-related protein (AngII protein) in HUVEC cells protected by NC8-pSIP409-alr-ACEIP decreased and expression of ACE2 protein increased. These plantarum results suggested that NC8-pSIP409-alr-ACEIP protects against H2O2-induced injury in HUVEC cells. The mechanism for this effect is related to enhancement of antioxidant capacity and apoptosis.


方法:首次构建以双基因标记的非抗性筛选锚定为表达载体的新功能型植物乳杆菌(NC8-pSIP409-alr-ACEIP),使用不同浓度的过氧化氢(H2O2)诱导人脐静脉内皮细胞(HUVEC)细胞,构建氧化应激损伤细胞模型(Hy-HUVEC),通过MTT、流式细胞术等方法检测Hy-HUVEC的凋亡,通过蛋白质印迹法(western blot)检测AngII、ACE2、诱导型一氧化氮合酶(iNOS)、尼克酰胺腺嘌呤二核苷酸磷酸氧化酶(gp91phox)、Bcl-2和Bax的蛋白表达情况,同时检测氧化应激相应指标活性氧(ROS)、线粒体膜电位(MMP)、丙二醛(MDA)和超氧化物歧化酶(SOD)的变化情况。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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