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Journal of Zhejiang University SCIENCE A 2007 Vol.8 No.9 P.1469-1474


Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs

Author(s):  WU Dong-lei, ZHENG Ping, MAHMOOD Qaisar, YANG Xiao-sheng

Affiliation(s):  Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   wudl@zju.edu.cn, pzheng@zju.edu.cn

Key Words:  Denitrifying bacteria, Arthrobacter sp. strain CW-1, Dimethyl phthalate (DMP), Phthalic acid (PA), DMP degradation pathway

WU Dong-lei, ZHENG Ping, MAHMOOD Qaisar, YANG Xiao-sheng. Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs[J]. Journal of Zhejiang University Science A, 2007, 8(9): 1469-1474.

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author="WU Dong-lei, ZHENG Ping, MAHMOOD Qaisar, YANG Xiao-sheng",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs
%A WU Dong-lei
%A YANG Xiao-sheng
%J Journal of Zhejiang University SCIENCE A
%V 8
%N 9
%P 1469-1474
%@ 1673-565X
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.A1469

T1 - Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs
A1 - WU Dong-lei
A1 - ZHENG Ping
A1 - MAHMOOD Qaisar
A1 - YANG Xiao-sheng
J0 - Journal of Zhejiang University Science A
VL - 8
IS - 9
SP - 1469
EP - 1474
%@ 1673-565X
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.A1469

Isolation of new bacterial strains and recognition of their metabolic activities are highly desirable for sustainability of natural ecosystems. Biodegradation of dimethyl phthalate (DMP) under anoxic conditions has been shown to occur as a series of sequential steps using strain CW-1 isolated from digested sludge of Sibao Wastewater Treatment Plant in Hangzhou, China. The microbial colony on LB medium was yellowish, 3~5 mm in diameter, convex in the center, and embedded in mucous externally. The individual cells of strain CW-1 are irregular rods, measuring (0.6~0.7)×(0.9~1.0) μm, V-shaped, with clubbed ends, Gram positive and without any filaments. 16S rDNA (1438 bp) sequence analysis showed that the strain was related to Arthrobacter sp. CW-1 and can degrade PAEs utilizing nitrate as electron acceptor, but cannot mineralize DMP completely. The degradation pathway was recommended as: dimethyl phthalate (DMP)→monomethyl phthalate (MMP)→phthalic acid (PA). DMP biodegradation was a first order reaction with degradation rate constant of 0.3033 d−1 and half-life 2.25 d. The DMP conversion to PA by CW-1 could be described by using sequential kinetic model.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Atlas, E., Giam, C.S., 1981. Global transport of organic pollutants: ambient concentrations in the remote marine atmosphere. Science, 211(4478):163-165.

[2] Bittrich, H.J., Haberland, D., Just, G., 1979. Method of Chemist-kinetic Calculate. Higher Education Press, Beijing, p.34-36 (in Chinese).

[3] Booker, S.M., 2001. NTP center reports on phthalate concerns. Environ. Health Persp., 109(6):A260-A261.

[4] Cartwright, C.D., Owen, S.A., Thompson, I.P., Burns, R.G., 2000. Biodegradation of diethyl phthalate in soil by a novel pathway. FEMS Microbiol. Lett., 186(1):27-34.

[5] Chang, B.V., Liao, C.S., Yuan, S.Y., 2005. Anaerobic degradation of diethyl phthalate, di-n-butyl phthalate, and di-(2-ethylhexyl) phthalate from river sediment in Taiwan. Chemosphere, 58(11):1601-1607.

[6] Chatterjee, S., Dutta, T.K., 2003. Metabolism of butyl phthalate by Gordonia sp. strain MTCC 4818. Biochemical and Biophysical Research Communications, 309(1):36-43.

[7] Koch, H.M., Drexler, H., Angerer, J., 2003. An estimation of the daily intake of di-(2-ethylhexyl) phthalate (DEHP) and other phthalates in the general population. Int. J. Hyg. Environ. Health, 206(2):77-83.

[8] Levén, L., Schnürer, A., 2005. Effects of temperature on biological degradation of phenols, benzoates and phthalates under metnanogeneic conditions. Int. Biodeter. Biodegr., 55(2):153-160.

[9] Li, W.Q., Zhang, M., Li, H.F., Zan, L.S., 2002. The study of soil status in fields under plastic house gardening. ACTA Pedologica Sinica, 39:283-287 (in Chinese).

[10] Qian, Z.S., Min, H., 1986. Methane Ferment Microbiology. Zhejiang Science and Technology Press, Hangzhou, p.50-67 (in Chinese).

[11] Ruan, A.D., Min, H., Peng, X.H., Huang, Z., 2005. Isolation and characterization of Pseudomonas sp. strain HF-1, capable of degrading nicotine. Res. Microbio., 156(5-6):700-706.

[12] Shen, P., Wang, Y.Y., Gu, J.D., 2004. Degradation of phthalate acid and ortho-dimethyl phthalate ester by bacteria isolated from sewage sludge and its biochemical pathway. Chin. J. Appl. Environ. Biol., 10:643-646 (in Chinese).

[13] Staples, C.A., Peterson, D.R., Parkerton, T.F., Adams, W.J., 1997. The environmental fate of phthalate esters: A literature review. Chemosphere, 35(4):667-749.

[14] Vikelsøe, J., Thomsen, M., Carlsen, L., 2002. Phthalate and nonylphenols in profiles of differently dressed soils. Sci. Total Environ., 296(1-3):105-116.

[15] Wang, J.L., Liu, P., Qian, Y., 1999. Microbial metabolism of di-butyl phthalate (DBP) by a denitrifying bacterium. Process Biochem., 34(6-7):745-749.

[16] Weisburg, W.G., Barns, M.S., Pelletier, A.D., Lane. J.D., 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol., 173:697-703.

[17] Wezel, V., Van, V., Posthumus, R., 2000. Environmental risk limits for two phthalates, with special emphasis on endocrine disruptive properties. Ecotoxico. and Environ. Safety, 46:3205-3214.

[18] Wilson, K., 1997. Preparation of Genomic DNA from Bacteria. In: Ausubel, F.M., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (Eds.), Current Protocols in Molecular Biology. Wiley, New York, p.241-245.

[19] Xia, F.Y., Zheng, P., Zhou, Q., Feng, X.S., 2002. Shake flask biodegradation of phthalic acid esters. ACTA Scienctiae Circumstantiae, 22:379-1384 (in Chinese).

[20] Xu, X.R., Li, H.B., Gu, J.D., 2005. Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate ester by Pseudomonas fluorescens B-1. Int. Biodeter. Biodegr., 55(1):9-15.

[21] Zheng, P., 2005. Laboratory Manual for Environmental Microbiology. Zhejiang University Press, Hangzhou, p.67-68 (in Chinese).

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