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Received: 2006-01-16

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.2 P.116-120


Evaluation of organ distribution of microcystins in the freshwater phytoplanktivorous fish Hypophthalmichthys molitrix

Author(s):  ZHANG Hang-jun, ZHANG Jian-ying, HONG Ye, CHEN Ying-xu

Affiliation(s):  School of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China

Corresponding email(s):   zjy@zju.edu.cn

Key Words:  Microcystin, Organ distribution, Bioaccumulation, Fish, Risk

ZHANG Hang-jun, ZHANG Jian-ying, HONG Ye, CHEN Ying-xu. Evaluation of organ distribution of microcystins in the freshwater phytoplanktivorous fish Hypophthalmichthys molitrix[J]. Journal of Zhejiang University Science B, 2007, 8(2): 116-120.

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author="ZHANG Hang-jun, ZHANG Jian-ying, HONG Ye, CHEN Ying-xu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%A ZHANG Jian-ying
%A CHEN Ying-xu
%J Journal of Zhejiang University SCIENCE B
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%P 116-120
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0116

T1 - Evaluation of organ distribution of microcystins in the freshwater phytoplanktivorous fish Hypophthalmichthys molitrix
A1 - ZHANG Hang-jun
A1 - ZHANG Jian-ying
A1 - HONG Ye
A1 - CHEN Ying-xu
J0 - Journal of Zhejiang University Science B
VL - 8
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EP - 120
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2007.B0116

To evaluate the public health risk of exposure to microcystins in fish food in China, the distribution pattern of microcystin-LR and microcystin-RR in various organs (liver, intestine, kidney, muscle and lipid) of the dominant freshwater phytoplanktivorous fish Hypophthalmichthys molitrix in Hangzhou, China’s Tiesha River was investigated with the method of HPLC-ESI-MS analysis. The distribution of microcystins was different in the fish organs and the major total microcystins (microcystin-LR and microcystin-RR) were present in the intestines (6.49 μg/g fresh weight), followed by the livers (4.52 μg/g fresh weight) and the muscles (2.86 μg/g fresh weight). microcystins were detected in kidneys (1.35 μg/g fresh weight), but not detected in lipid. The results suggested that the mean daily intake from fish was 0.03 μg/kg body weight which was very close to the recommended WHO tolerable daily intake (TDI) level of 0.04 μg/kg body weight per day, and local people were warned they may have health risk if they consumed fish from the river.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Barco, M., Rivera, J., Caixach, J., 2002. Analysis of cyanobacterial hepatotoxins in water samples by microbore reversed-phase liquid chromatography-electrospray ionization mass spectrometry. J. Chromatogr. A, 959(1-2):103-111.

[2] Chorus, I., Bartram, J., 1999. Toxic Cyanobacteria in Water. A Guide to Public Health Consequences, Monitoring and Management. E and FN Spon, London, p.416-417.

[3] Dawson, R.M., 1998. The toxicology of microcystins. Toxicon, 36(7):953-962.

[4] de Maagd, P.G.J., Hendriks, A.J., Seinen, W., Sijm, D.T.H.M., 1999. pH-dependent hydrophobicity of the cyanobacteria toxin microcystin-LR. Water Res., 33(3):677-680.

[5] de Magalhães, V.F., Soares, R.M., Azevedo, S.M.F.O., 2001. Microcystin contamination in fish from the Jacarepaguá Lagoon (Rio de Janeiro, Brazil): ecological implication and human health risk. Toxicon, 39(7):1077-1085.

[6] Falconer, L.R., Burch, M.D., Steffensen, D.A., Choice, M., Coverdale, O.R., 1994. Toxicity of the blue-green alga (cyanobacterium) Microcystis aeruginosa in drinking water to growing pigs, as an animal model for human injury and risk assessment. Environ. Toxicol. Water Qual., 9(2):131-139.

[7] Gupta, N., Pant, S.C., Vijayaraghavan, R., Lakshmana, R.P.V., 2003. Comparative toxicity evaluation of cyanobacterial cyclic peptide toxin microcystins variants (LR, RR, YR) in mice. Toxicology, 188(2-3):285-296.

[8] Harada, K.I., Tsuji, K., Watanabe, M.F., Kondo, F., 1996. Stability of microcystins from cyanobacteria. III. Effect of pH and temperature. Phycologia, 35(6):83-88.

[9] Huynh-Delerme, C., Edery, M., Huet, H., Puiseux-Dao, S., Bernard, C., Fontaine, J.J., Crespeau, F., de Luze, A., 2005. Microcystin-LR and embryo-larval development of medaka fish, Oryzias latipes. I. Effects on the digestive tract and associated systes. Toxicon, 46(1):16-23.

[10] Ibelings, B.W., Bruning, K., de Jonge, J., Wolfstein, K., Dionisio, P.L.M., Postma, J., Burger, T., 2005. Distribution of microcystins in a lake foodweb: no evidence for biomagnification. Microbial Ecol., 49(4):487-500.

[11] Malbrouck, C., Trausch, G., Devos, P., Kestemont, P., 2003. Hepatic accumulation and effects of microcystin-LR on juvenile goldfish Carassius auratus L. Comp. Biochem. Physiol. Part C, 135(1):39-48.

[12] Mohamed, Z.A., Carmichael, W.W., Hussein, A.A., 2003. Estimation of microcystins in the freshwater fish Oreochromisniloticus in an Egyptian fish farm containing a Microcystis bloom. Environ. Toxicol., 18(2):137-141.

[13] Rivasseau, C., Martine, S., Hennion, M.C., 1998. Determination of some physicochemical parameters of microcystins (cyanobacterial toxins) and trace level analysis in environmental samples using liquid chromatography. J. Chromatogr. A, 799(1-2):155-169.

[14] Specziár, A., Tölg, L., Biró, P., 1997. Feeding strategy and growth of cyprinids in the littoral zone of Lake Balaton. J. Fish Biol., 51(6):1109-1124.

[15] Tencalla, F., Dietrich, D., Schlatter, C., 1994. Toxicity of Microcystis aeruginosa peptide toxins to yearling rainbow trout (Oncorhynchus mykiss). Aquat. Toxicol., 30(3):215-224.

[16] Wang, X.F., Guo, Z.L., Wang, Y., 2003. Detection of microcystin class compounds in water by colorimetric protein phosphatases inhibition assay. Acta Hydrobiologica Sinica, 27(4):431-433 (in Chinese).

[17] Xie, L.Q., Xie, P., Ozawa, K., Honma, T., Yokoyama, A., Park, H.D., 2004. Dynamics of microcystins-LR and -RR in the phytoplanktivorous silver carp in a sub-chronic toxicity experiment. Environ. Pollut., 127(3):431-439.

[18] Xie, L.Q., Xie, P., Guo, L.G., Li, L., Miyabara, Y., Park, H.D., 2005. Organ distribution and bioaccumulation of microcystins in freshwater fish at different trophic levels from the eutrophic lake Chaohu, China. Environ. Toxicol., 20(3):293-300.

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