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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.3 P.162-169

http://doi.org/10.1631/jzus.2007.B0162


Detection of PCV2 DNA by SYBR Green I-based quantitative PCR


Author(s):  YANG Zong-zhao, HABIB Mudasser, SHUAI Jiang-bing, FANG Wei-huan

Affiliation(s):  Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   whfang@zju.edu.cn

Key Words:  Porcine circovirus type 2 (PCV2), Real-time PCR, Sensitivity


YANG Zong-zhao, HABIB Mudasser, SHUAI Jiang-bing, FANG Wei-huan. Detection of PCV2 DNA by SYBR Green I-based quantitative PCR[J]. Journal of Zhejiang University Science B, 2007, 8(3): 162-169.

@article{title="Detection of PCV2 DNA by SYBR Green I-based quantitative PCR",
author="YANG Zong-zhao, HABIB Mudasser, SHUAI Jiang-bing, FANG Wei-huan",
journal="Journal of Zhejiang University Science B",
volume="8",
number="3",
pages="162-169",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0162"
}

%0 Journal Article
%T Detection of PCV2 DNA by SYBR Green I-based quantitative PCR
%A YANG Zong-zhao
%A HABIB Mudasser
%A SHUAI Jiang-bing
%A FANG Wei-huan
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 3
%P 162-169
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0162

TY - JOUR
T1 - Detection of PCV2 DNA by SYBR Green I-based quantitative PCR
A1 - YANG Zong-zhao
A1 - HABIB Mudasser
A1 - SHUAI Jiang-bing
A1 - FANG Wei-huan
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 3
SP - 162
EP - 169
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0162


Abstract: 
We developed an assay for the detection and quantitation of porcine circovirus type 2 (PCV2) with the SYBR Green I-based real-time PCR. The real-time PCR provides a broad dynamic range, detecting from 103 to 1011 copies of DNA per reaction. No cross-reactions were found in specimens containing PCV1. Because of the high sensitivity and specificity of the assay with a relatively rapid and simple procedure, real-time PCR can be used as a routine assay for the clinical diagnosis of PCV2 infection. In this study we applied real-time PCR assay to 80 clinical samples, collected from 40 pigs with postweaning multisystemic wasting syndrome (PMWS) and 40 healthy pigs in comparison with conventional PCR assay. In 56 of 80 samples, PCV2 DNA was detected by conventional PCR assay. All samples positive for PCV2 DNA in conventional PCR assay were also positive in real-time assay, and 12 of 24 samples that tested negative for PCV2 DNA in the conventional assay were tested positive in real-time PCR assay. real-time PCR assay increased the number of samples in which PCV2 was detected by 15%. It is, therefore, considered to be a useful tool for the detection of PCV2.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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