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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.4 P.260-265

http://doi.org/10.1631/jzus.2007.B0260


Mrakia psychrophila sp. nov., a new species isolated from Antarctic soil


Author(s):  XIN Ming-xiu, ZHOU Pei-jin

Affiliation(s):  College of Life Sciences, Beijing Normal University, Beijing 100875, China; more

Corresponding email(s):   xinmingxiu@bnu.edu.cn

Key Words:  Mrakia psychrophila, Psychrophilic yeast, 18S rDNA sequence, Teliospores


XIN Ming-xiu, ZHOU Pei-jin. Mrakia psychrophila sp. nov., a new species isolated from Antarctic soil[J]. Journal of Zhejiang University Science B, 2007, 8(4): 260-265.

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author="XIN Ming-xiu, ZHOU Pei-jin",
journal="Journal of Zhejiang University Science B",
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pages="260-265",
year="2007",
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doi="10.1631/jzus.2007.B0260"
}

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%DOI 10.1631/jzus.2007.B0260

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T1 - Mrakia psychrophila sp. nov., a new species isolated from Antarctic soil
A1 - XIN Ming-xiu
A1 - ZHOU Pei-jin
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DOI - 10.1631/jzus.2007.B0260


Abstract: 
The yeast strain (Y18) was isolated from a soil sample collected from Fildes Peninsula, Antarctica. The strain is a psychrophilic yeast with optimum and maximum growth temperatures of 10 °C and 18 °C, respectively. teliospores were formed after 7 d on malt agar, when the germination of teliospores was observed. Both inositol and D-glucuronate were assimilated. Positive results of the DBB (diazonium blue B) color reaction, urease test, and starch formation were observed. The major CoQ is Q8. All results indicated that Y18 belongs to the genes of Mrakia. The 18S rDNA sequence analyses showed that Y18 is closely related to Mrakia frigida. DNA-DNA relatedness study, and some biochemistry characteristics indicated that Y18 represents a new species for which Mrakia psychrophila sp. nov. is proposed.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Babeva, I.P., Lisichkina, G.A., Reshetova, I.S., Danilevich, V.N., 2002. Mrakia curviuscula sp. nov.: a new species of psychrophilic yeasts from forest substrates. Mikrobiologiia, 71(4):526-532.

[2] D'Amico, S., Collins, T., Marx, J.C., Feller, G., Gerday, C., 2006. Psychrophilic microorganisms: challenges for life. EMBO Rep., 7(4):385-389.

[3] Diaz, M.R., Fell, J.W., 2000. Molecular analyses of the IGS & ITS regions of rDNA of the psychrophilic yeasts in the genus Mrakia. Antonie van Leeuwenhoek, 77(1):7-12.

[4] Fell, J.W., Statzell-Tallman, A.C., Hunter, I.L., Phaff, H.J., 1969. Leucosporidium gen. n., the heterobasidiomycetous stage of several yeasts of the genus Candida. Antonie van Leeuwenhoek, 35(1):433-462.

[5] Kourkoutas, Y., Koutinas, A., Kanellaki, M., Banat, I., Marchant, R., 2002. Continuous wine fermentation using a psychrophilic yeast immobilized on apple cuts at different temperatures. Food Microbiol., 19(2-3):127-134.

[6] Lambo, A., Patel, T., 2006. Cometabolic degradation of polychlorinated biphenyls at low temperature by psychrotolerant bacterium Hydrogenophaga sp. IA3-A. Curr. Microbiol., 53(1):48-52.

[7] Liu, D., Schmid, R., Rusnak, M., 2006. Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst. Appl. Microbiol. Biotechnol., 72(5):1024-1032.

[8] López-Archilla, A., Gonzalez, A., Terron, M., Amils, R., 2004. Ecological study of the fungal populations of the acidic Tinto River in southwestern Spain. Can. J. Microbiol., 50(11):923-934.

[9] Margesin, R., Fauster, V., Fonteyne, P.A., 2005. Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida. Lett. Appl. Microbiol., 40(6):453-459.

[10] Nakagawa, T., Nagaoka, T., Taniguchi, S., Miyaji, T., Tomizuka, N., 2004. Isolation and characterization of psychrophilic yeasts producing cold-adapted pectinolytic enzymes. Lett. Appl. Microbiol., 38(5):383-387.

[11] Nakase, T., Suzuki, M., 1988. Sporobolomyces yuccicola, a new species of ballistosporous yeast equipped with ubiquinone-9. Antonie van Leeuwenhoek, 54(1):47-55.

[12] Pfeffer, J., Richter, S., Nieveler, J., Hansen, C.E., Rhlid, R., Schmid, R., Rusnak, M., 2006. High yield expression of lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterization. Appl. Microbiol. Biotechnol., 72(5):931-938.

[13] Ruan, J.S., Liu, Z.H., Li, H., Liang, L.N., Yang, D.C., 1990. The Development and Application of Actinomyces. Science Press, Beijing, p.202-206.

[14] Ruberto, L., Vazquez, S., Lobalbo, A., Maccormack, W.P., 2005. Psychrotolerant hydrocarbon-degrading Rhodococcus strains isolated from polluted Antarctic soils. Antarctic Sci., 17(1):47-56.

[15] Sabri, A., Bare, G., Jacques, P., 2001. Influence of moderate temperatures on myristoyl-CoA metabolism and acyl-CoA thioesterase activity in the psychrophilic antarctic yeast Rhodotorula aurantiaca. J. Biol. Chem., 276(16):12691-12696.

[16] Scorzetti, G., Petrescu, L., Yarrow, D., Fell, J.W., 2000. Cryptococcus adeliensis sp. nov., a xylanase producing basidiomycetous yeast from Antarctica. Antonie van Leeuwenhoek, 77(2):153-157.

[17] Takashima, M., Nakase, T., 1996. A phylogenetic study of the genus Tilletiopsis, Tilletiaria anomala and related taxa based on the small subunit ribosomal DNA sequences. J. Gen. Appl. Microbiol., 42:421-429.

[18] Thompson, J.D., Higgins, D.G., Gibson, T.J., 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic. Acids Res., 22(22):4673-4680.

[19] van de Peer, Y., de Wachter, R., 1994. Treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. Applic. Biosci., 10:569-570.

[20] van der Walt, J.P., Yarrow, D., 1984. Methods for the Isolation, Maintenance, Classification and Identification of Yeasts. In: Kreger-van Rij, N.J.W. (Ed.), The Yeasts: A Taxonomic Study, 3rd Ed. Elsevier Sci. Publ., Amsterdam, p.45-105.

[21] van Uden, N., 1984. Temperature profiles of yeast. Adv. Microb. Physiol., 25:195-251.

[22] Welander, U., 2005. Microbial degradation of organic pollutants in soil in a cold climate. Soil Sediment Contam., 14(3):281-291.

[23] Yamada, Y., Kondo, K., 1973. Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium. J. Gen. Appl. Microbiol., 19:59-77.

[24] Yamada, Y., Komagata, K., 1987. Mrakia gen. nov., a heterobasidiomycetous yeast genus for the Q8-equipped, self-sporulating organisms which produce a unicellular metabasidium, formerly classified in the genus Leucosporidium. J. Gen. Appl. Microbiol., 33:455-457.

[25] Zhu, H., Qu, F., Zhu, L.H., 1993. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic. Acids Res., 21(22):5279-5280.

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