JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE A 2014 Vol.15 No.8 P.618-623

Random amplified polymorphic DNA reveals that TiO₂ nanoparticles are genotoxic to Cucurbita pepo *

Author(s): Fabiola Moreno-Olivas^1,4, Vincent U. Gant Jr.², Kyle L. Johnson², Jose R. Peralta-Videa^1,3,4, Jorge L. Gardea-Torresdey^1,3,4
Affiliation(s): 1. Depertment of Chemistry, The University of Texas at El Paso, El Paso, TX 79968, USA; more
Corresponding email(s): fmoreno2@miners.utep.edu
Key Words: Random amplified polymorphic DNA (RAPD), Titanium dioxide (TiO₂), Nanoparticles (NPs), Genomic DNA, Zucchini

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Fabiola Moreno-Olivas, Vincent U. Gant Jr. , Kyle L. Johnson, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey. Random amplified polymorphic DNA reveals that TiO₂ nanoparticles are genotoxic to Cucurbita pepo[J]. Journal of Zhejiang University Science A, 2014, 15(8): 618-623.

@article{title="Random amplified polymorphic DNA reveals that TiO₂ nanoparticles are genotoxic to Cucurbita pepo",
author="Fabiola Moreno-Olivas, Vincent U. Gant Jr. , Kyle L. Johnson, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey",
journal="Journal of Zhejiang University Science A",
volume="15",
number="8",
pages="618-623",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.A1400159"
}

%0 Journal Article
%T Random amplified polymorphic DNA reveals that TiO₂ nanoparticles are genotoxic to Cucurbita pepo
%A Fabiola Moreno-Olivas
%A Vincent U. Gant Jr.
%A Kyle L. Johnson
%A Jose R. Peralta-Videa
%A Jorge L. Gardea-Torresdey
%J Journal of Zhejiang University SCIENCE A
%V 15
%N 8
%P 618-623
%@ 1673-565X
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A1400159

TY - JOUR
T1 - Random amplified polymorphic DNA reveals that TiO₂ nanoparticles are genotoxic to Cucurbita pepo
A1 - Fabiola Moreno-Olivas
A1 - Vincent U. Gant Jr.
A1 - Kyle L. Johnson
A1 - Jose R. Peralta-Videa
A1 - Jorge L. Gardea-Torresdey
J0 - Journal of Zhejiang University Science A
VL - 15
IS - 8
SP - 618
EP - 623
%@ 1673-565X
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A1400159

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Titanium dioxide nanoparticles (TiO₂ NPs) are used in cosmetics, sunscreens, paints, and toothpaste, among other applications. These NPs are very stable and can be transported and dispersed in wastewater and biosolids. Animal species have shown negative reactions to TiO₂ NPs. However, little is known about their toxicity in plants, specifically the possibility of genotoxic effects. In this study, we used a random amplified polymorphic DNA (RAPD) technique to study the genotoxic effects of TiO₂ NPs on hydroponically cultivated zucchini (Cucurbita pepo) plants. Seeds were allowed to germinate for 7 d and plants were selected at random for individual and population studies. Four plants were selected for the individual study and 18 for the population study. RAPD profiles of TiO₂ NPs treated plants showed differences in band intensity, loss of bands, or appearance of new bands, compared to untreated plants. To the authors’ knowledge, this is the first report of the genotoxic potential of TiO₂ NPs in zucchini.

随机扩增多态性DNA技术研究发现二氧化钛纳米颗粒对西葫芦具有基因毒性

研究目的：二氧化钛（TiO2）纳米颗粒已经广泛应用于化妆品、防晒霜、涂料和牙膏等。这些纳米颗粒性质非常稳定，能在废水和生物固体中转移和分散。现有研究表明，TiO2纳米颗粒对动物正常生理活动具有毒性等负面作用。但是，它们对植物是否具有毒性特别是是否会产生植物基因毒性至今尚不清楚。因此，本文使用随机扩增多态性DNA技术研究TiO2纳米颗粒是否对西葫芦具有基因毒性，为TiO2纳米颗粒排放进入环境后的潜在植物毒性风险评价提供依据。
创新要点：首次发现了TiO2纳米颗粒对西葫芦具有基因毒性。
重要结论：采用随机扩增多态性DNA技术，发现TiO2纳米颗粒污染处理的西葫芦样品与未处理样品的基因组DNA图谱相比，不仅在谱带强度有明显差异，而且存在谱带消失和新谱带产生现象，表明TiO2纳米颗粒对西葫芦具有基因毒性。
随机扩增多态性DNA技术；TiO2；纳米颗粒；基因组DNA；西葫芦；毒性

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随机扩增多态性DNA技术研究发现二氧化钛纳米颗粒对西葫芦具有基因毒性

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