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CLC number: TQ926.4; O657; Q939.97

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Received: 2006-05-18

Revision Accepted: 2006-07-31

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.1 P.51-59


Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma

Author(s):  Hui NI, Qi-he CHEN, Guo-qing HE, Guang-bin WU, Yuan-fan YANG

Affiliation(s):  School of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   nihui1973@yahoo.com.cn, gqhe@zju.edu.cn

Key Words:  Astaxanthin, Phaffia rhodozyma, Extracting, Optimization, Acidic method

Hui NI, Qi-he CHEN, Guo-qing HE, Guang-bin WU, Yuan-fan YANG. Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma[J]. Journal of Zhejiang University Science B, 2008, 9(1): 51-59.

@article{title="Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma",
author="Hui NI, Qi-he CHEN, Guo-qing HE, Guang-bin WU, Yuan-fan YANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma
%A Hui NI
%A Qi-he CHEN
%A Guo-qing HE
%A Guang-bin WU
%A Yuan-fan YANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 1
%P 51-59
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B061261

T1 - Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma
A1 - Hui NI
A1 - Qi-he CHEN
A1 - Guo-qing HE
A1 - Guang-bin WU
A1 - Yuan-fan YANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 1
SP - 51
EP - 59
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B061261

optimization of a process for extracting astaxanthin from Phaffia rhodozyma by acidic method was investigated, regarding several extraction factors such as acids, organic solvents, temperature and time. Fractional factorial design, central composite design and response surface methodology were used to derive a statistically optimal model, which corresponded to the following optimal condition: concentration of lactic acid at 5.55 mol/L, ratio of ethanol to yeast dry weight at 20.25 ml/g, temperature for cell-disruption at 30 °C, and extraction time for 3 min. Under this condition, astaxanthin and the total carotenoids could be extracted in amounts of 1294.7 μg/g and 1516.0 μg/g, respectively. This acidic method has advantages such as high extraction efficiency, low chemical toxicity and no special requirement of instruments. Therefore, it might be a more feasible and practical method for industrial practice.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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