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CLC number: Q946

On-line Access: 2015-08-04

Received: 2014-11-11

Revision Accepted: 2015-04-30

Crosschecked: 2015-07-08

Cited: 6

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Citations:  Bibtex RefMan EndNote GB/T7714


Rowland Monday Ojo Kayode


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.8 P.680-689


Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds

Author(s):  Rowland Monday Ojo Kayode, Anthony Jide Afolayan

Affiliation(s):  Medicinal Plant and Economic Development Research Centre, Department of Botany, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa

Corresponding email(s):   kayodermosnr@gmail.com, AAfolayan@ufh.ac.za

Key Words:  Moringa oleifera seed, Extraction methods, Essential oil, Cytotoxicity

Rowland Monday Ojo Kayode, Anthony Jide Afolayan. Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds[J]. Journal of Zhejiang University Science B, 2015, 16(8): 680-689.

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author="Rowland Monday Ojo Kayode, Anthony Jide Afolayan",
journal="Journal of Zhejiang University Science B",
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%0 Journal Article
%T Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds
%A Rowland Monday Ojo Kayode
%A Anthony Jide Afolayan
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 8
%P 680-689
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400303

T1 - Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds
A1 - Rowland Monday Ojo Kayode
A1 - Anthony Jide Afolayan
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 8
SP - 680
EP - 689
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400303

Renewed interest in natural materials as food flavors and preservatives has led to the search for suitable essential oils. Moringa oleifera seed essential oil was extracted by solvent-free microwave and hydrodistillation. This study assessed its chemical constituents. cytotoxicity of the oils was investigated using hatchability and lethality tests on brine shrimps. A total of 16 and 26 compounds were isolated from the hydrodistillation extraction (HDE) and solvent-free microwave extraction (SME) oils, respectively, which accounted for 97.515% and 97.816% of total identifiable constituents, respectively. At 24 h when the most eggs had hatched, values of the SME (56.7%) and HDE (60.0%) oils were significantly different (P<0.05) from those of sea water (63.3%) and chloramphenicol (15.0%). Larva lethality was different significantly (P<0.05) between HDE and SME oils at different concentrations and incubation periods. The median lethal concentration (LC50) of the oils was >1000 mg/ml recommended as an index for non-toxicity, which gives the oil advantage over some antioxidant, antimicrobial, therapeutic, and preservative chemicals.


结论:HDE和SME两种方法提取的辣木籽精油分别含有16和26种化学成分,各自占总可识别成分的97.515%和97.816%。因此,SME提取法含有更多的成分。盐水虾卵分别在含有两种精油、海水和氯霉素的样品中孵化24 h后,孵化率分别为60.0%(HDE)、56.7%(SME)、63.3%(海水)和15.0%(氯霉素),精油中的孵化率远高于含有氯霉素的样品的孵化率(P<0.05)。同时,SME和HDE精油对幼虾的半致死量分别为2908.23和3473.63 µg/ml(大于1000 µg/ml被认为无毒)。因此,两种方法提取的精油可广泛适用于药物、食品防腐剂、抗氧化剂和香料等添加剂。


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