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CLC number: X17

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Received: 2008-01-19

Revision Accepted: 2008-04-26

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.9 P.707-712


Availability and toxicity of Fe(II) and Fe(III) in Caco-2 cells

Author(s):  Wan-ling HE, Ying FENG, Xiao-li LI, Yan-yan WEI, Xiao-e YANG

Affiliation(s):  Ministry of Education Key Laboratory of Polluted Environmental Remediation and Ecological Health, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   xyang@zju.edu.cn

Key Words:  Iron availability, Caco-2 cells, Fe(II), Fe(III), Toxicity

Wan-ling HE, Ying FENG, Xiao-li LI, Yan-yan WEI, Xiao-e YANG. Availability and toxicity of Fe(II) and Fe(III) in Caco-2 cells[J]. Journal of Zhejiang University Science B, 2008, 9(9): 707-712.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Availability and toxicity of Fe(II) and Fe(III) in Caco-2 cells
%A Wan-ling HE
%A Ying FENG
%A Xiao-li LI
%A Yan-yan WEI
%A Xiao-e YANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 9
%P 707-712
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820023

T1 - Availability and toxicity of Fe(II) and Fe(III) in Caco-2 cells
A1 - Wan-ling HE
A1 - Ying FENG
A1 - Xiao-li LI
A1 - Yan-yan WEI
A1 - Xiao-e YANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 9
SP - 707
EP - 712
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820023

The objective of the present study was to compare the toxicity and availability of fe(II) and fe(III) to caco-2 cells. Cellular damage was studied by measuring cell proliferation and lactate dehydrogenase (LDH) release. The activities of two major antioxidative enzymes [superoxide dismutase (SOD) and glutathione peroxidase (GPx)] and differentiation marker (alkaline phosphatase) were determined after the cells were exposed to different levels of iron salts. The cellular iron concentration was investigated to evaluate iron bioavailability. The results show that iron uptake of the cells treated with fe(II) is significantly higher than that of the cells treated with fe(III) (P<0.05). fe(II) at a concentration >1.5 mmol/L was found to be more effective in reducing cellular viability than fe(III). LDH release investigation suggests that fe(II) can reduce stability of the cell membrane. The activities of SOD and GPx of the cells treated with fe(II) were higher than those of the cells treated with fe(III), although both of them increased with raising iron supply levels. The results indicate that both fe(II) and fe(III) could reduce the cellular antioxidase gene expression at high levels.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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