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On-line Access: 2010-04-28

Received: 2009-12-03

Revision Accepted: 2010-03-04

Crosschecked: 2010-04-07

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.5 P.307-314


Morphologic observation and classification criteria of atretic follicles in guinea pigs

Author(s):  Wei Wang, Hong-lin Liu, Wei Tian, Fen-fen Zhang, Yan Gong, Jin-wei Chen, Da-gan Mao, Fang-xiong Shi

Affiliation(s):  College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Corresponding email(s):   fxshi@njau.edu.cn

Key Words:  Guinea pig, Follicular atresia, Classification criteria

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Wei Wang, Hong-lin Liu, Wei Tian, Fen-fen Zhang, Yan Gong, Jin-wei Chen, Da-gan Mao, Fang-xiong Shi. Morphologic observation and classification criteria of atretic follicles in guinea pigs[J]. Journal of Zhejiang University Science B, 2010, 11(5): 307-314.

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author="Wei Wang, Hong-lin Liu, Wei Tian, Fen-fen Zhang, Yan Gong, Jin-wei Chen, Da-gan Mao, Fang-xiong Shi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Morphologic observation and classification criteria of atretic follicles in guinea pigs
%A Wei Wang
%A Hong-lin Liu
%A Wei Tian
%A Fen-fen Zhang
%A Yan Gong
%A Jin-wei Chen
%A Da-gan Mao
%A Fang-xiong Shi
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 5
%P 307-314
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900391

T1 - Morphologic observation and classification criteria of atretic follicles in guinea pigs
A1 - Wei Wang
A1 - Hong-lin Liu
A1 - Wei Tian
A1 - Fen-fen Zhang
A1 - Yan Gong
A1 - Jin-wei Chen
A1 - Da-gan Mao
A1 - Fang-xiong Shi
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 5
SP - 307
EP - 314
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900391

There is a lack of appropriate classification criteria for the determination of atretic follicles in guinea pigs. In the present study, new criteria were established based on the latest morphologic criteria for cell death proposed by the Nomenclature Committee on Cell Death (NCCD) in 2009. Ovaries of guinea pigs were sampled on different stages of estrous cycle, and the morphologic observations of atretic follicles were investigated in serial sections. The results showed that the process of follicular atresia could be classified into four continuous stages: (1) the granulosa layer became loose, and some apoptotic bodies began to appear; (2) the granulosa cells were massively eliminated; (3) the theca interna cells differentiated; and (4) the residual follicular cells degenerated. In addition, the examination revealed that these morphologic criteria were accurate and feasible. In conclusion, this study provides new criteria for the classification of atretic follicles in guinea pigs, and this knowledge can inform future research in the area.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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2010-09-29 01:21:56

articule very important and necesary

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