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Received: 2006-04-14

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.1 P.39-44


FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs

Author(s):  ZHOU Zhen-qi, WANG Tian, PAN Ling-mei, HUANG Rui-hua, SHI Fang-xiong

Affiliation(s):  College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Corresponding email(s):   fxshi@njau.edu.cn

Key Words:  Forkhead, FoxO, Gastrointestinal tract, Immunohistochemistry, Swine

ZHOU Zhen-qi, WANG Tian, PAN Ling-mei, HUANG Rui-hua, SHI Fang-xiong. FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs[J]. Journal of Zhejiang University Science B, 2007, 8(1): 39-44.

@article{title="FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs",
author="ZHOU Zhen-qi, WANG Tian, PAN Ling-mei, HUANG Rui-hua, SHI Fang-xiong",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs
%A ZHOU Zhen-qi
%A WANG Tian
%A PAN Ling-mei
%A HUANG Rui-hua
%A SHI Fang-xiong
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 1
%P 39-44
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0039

T1 - FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs
A1 - ZHOU Zhen-qi
A1 - WANG Tian
A1 - PAN Ling-mei
A1 - HUANG Rui-hua
A1 - SHI Fang-xiong
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 1
SP - 39
EP - 44
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0039

forkhead box (Fox) proteins play critical roles in the regulation of differentiation, proliferation, immunity and aging of cells. Most studies on Fox proteins are limited to cultured cells and rodent. The aim of the current study is to detect by immunohistrochemistry whether foxO1, foxO3a and foxO4 proteins are localized in the stomach and intestine of the pig. The results showed that foxO4 exists in the mucosa in all parts of the stomach and intestine; foxO3a exists mainly in the lamina propria and muscularis of some parts. However, foxO1 is not detectable in all parts of the stomach and intestine. Collectively, the results of the present study indicate that there exists a distinct expression pattern of Fox proteins, and that foxO4 is a primary forkhead transcriptional factor localized in the gastrointestinal tracts of the pig.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Accili, D., Arden, K.C., 2004. FoxOs at the crossroads of cellular metabolism, differentiation, and transformation. Cell, 117(4):421-426.

[2] Arden, K.C., Biggs, W.H., 2002. Regulation of the FoxO family of transcription factors by phosphatidylinositol-3 kinase-activated signaling. Arch. Biochem. Biophys., 403(2):292-298.

[3] Barthélémy, C., Henderson, C.E., Pettmann, B., 2004. FoxO3a induces motoneuron death through the Fas pathway in cooperation with JNK. BMC Neurosci., 5(1):48.

[4] Burgering, B.M., Kops, G.J., 2002. Cell cycle and death control: long live forkheads. Trends Biochem. Sci., 27(7):352-360.

[5] Fernandez de Mattos, S., Essafi, A., Soeiro, I., Pietersen, A.M., Birkenkamp, K.U., Edwards, C.S., Martino, A., Nelson, B.H., Francis, J.M., Jones, M.C., et al., 2004. FoxO3a and BCR-ABL regulate cyclin D2 transcription through a STAT5/BCL6-dependent mechanism. Mol. Cell Biol., 24(22):10058-10071.

[6] Finnberg, N., El-Deiry, W.S., 2004. Activating FOXO3a, NF-kappaB and p53 by targeting IKKs: an effective multi-faceted targeting of the tumor-cell phenotype? Cancer Biol. Ther., 3(7):614-616.

[7] Fukuoka, M., Daitoku, H., Hatta, M., Matsuzaki, H., Umemura, S., Fukamizu, A., 2003. Negative regulation of forkhead transcription factor AFX (FoxO4) by CBP-induced acetylation. Int. J. Mol. Med., 12(4):503-508.

[8] Graff, P., Amellem, O., Seim, J., Stokke, T., Pettersen, E.O., 2005. The role of p27 in controlling the oxygen-dependent checkpoint of mammalian cells in late G1. Anticancer Res., 25(3B):2259-2267.

[9] Hoekman, M.F., Jacobs, F.M., Smidt, M.P., Burbach, J.P., 2006. Spatial and temporal expression of FoxO transcription factors in the developing and adult murine brain. Gene Expr. Patterns, 6(2):134-140.

[10] Hosaka, T., Biggs, W.H.3rd, Tieu, D., 2004. Disruption of forkhead transcription factor (FOXO) family members in mice reveals their functional diversification. Proc. Natl. Acad. Sci. USA, 101(9):2975-2980.

[11] Hu, M.C., Lee, D.F., Xia, W., Golfman, L.S., Ou-Yang, F., Yang, J.Y., Zou, Y., Bao, S., Hanada, N., Saso, H., et al., 2004. IkappaB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a. Cell, 117(2):225-237.

[12] Jacobs, F.M., van der Heide, L.P., Wijchers, P.J., Burbach, J.P., Hoekman, M.F., Smidt, M.P., 2003. FoxO6, a novel member of the FoxO class of transcription factors with distinct shuttling dynamics. J. Biol. Chem., 278(38):35959-35967.

[13] Kobayashi, Y., Furukawa-Hibi, Y., Chen, C., Horio, Y., Isobe, K., Ikeda, K., Motoyema, N., 2005. SIRT1 is critical regulator of FOXO-mediated transcription in response to oxidative stress. Int. J. Mol. Med., 16(2):237-243.

[14] Li, X., Jiang, Y., Wang, Z., Liu, G., Hutz, R.J., Liu, W., Shi, F., 2005a. Regulation of FoxO1 transcription factor by nitric oxide and cyclic GMP in cultured rat granulosa cells. Zool. Sci., 22(12):1339-1346.

[15] Li, X., Xie, Z., Shi, F., 2005b. Involvement of forkhead box proteins in apoptosis and oncogenesis. Prog. Biochem. Biophs., 32(7):600-606.

[16] Li, X., Xie, Z., Shi, F., 2006. FOXO in the regulation of differentiation, proliferation, immunity and aging of cells. Chin. J. Clin. Rehabil., 10(9):158-162.

[17] Liu, Y., Lehmann, M., 2006. FOXO-independent suppression of programmed cell death by the PI3K/Akt signaling pathway in Drosophila. Dev. Genes Evol., 216(9):531-535.

[18] Martinez-Gac, L., Marques, M., Garcia, Z., Campanero, M.R., Carrera, A.C., 2004. Control of cyclin G2 mRNA expression by forkhead transcription factors: novel mechanism for cell cycle control by phosphoinositide 3-kinase and forkhead. Mol. Cell. Biol., 24(5):2181-2189.

[19] Michl, P., Downward, J., 2005. PI3K/AKT signaling in gastrointestinal cancers. Z. Gastroenterol., 43(10):1133-1139.

[20] Seoane, J., Le, H.V., Shen, L., Anderson, S.A., Massague, J., 2004. Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation. Cell, 117(2):211-223.

[21] Shi, F., LaPolt, P.S., 2003. Relationship between FoxO1 protein levels and follicular development, atresia, and luteinization in the rat ovary. J. Endocrinol., 179(2):195-203.

[22] Skurk, C., Izumiya, Y., Maatz, H., Razeghi, P., Shiojima, I., Sandri, M., Sato, K., Zeng, L., Schiekofer, S., Pimentel, D., et al., 2005. The FoxO3a transcription factor regulates cardiac myocyte size downstream of AKT signaling. J. Biol. Chem., 280(21):20814-20823.

[23] Stahl, M., Dijkers, P.F., Kops, G.J., Lens, S.M., Coffer, P.J., Burgering, B.M., Medema, R.H., 2002. The forkhead transcription factor FoxO regulates transcription of p27Kip1 and Bim in response to IL-2. J. Immunol. (Baltimore, Md.: 1950), 168(10):5024-5031.

[24] Wang, T., Huo, Y.J., Shi, F., Xu, R.J., Hutz, R.J., 2005. Effects of intrauterine growth retardation on development of the gastrointestinal tract in neonatal pigs. Biol. Neonate, 88(1):66-72.

[25] Weinkove, D., Halstead, J.R., Gems, D., Divecha, N., 2006. Long-term starvation and ageing induce AGE-1/PI 3-kinase-dependent translocation of DAF-16/FoxO to the cytoplasm. BMC Biol., 4(1):1.

[26] Yamamura, Y., Lee, W.L., Inoue, K., Ida, H., Ito, Y., 2006. RUNX3 cooperates with FOXO3a to induce apoptosis in gastric cancer cells. J. Biol. Chem., 281(8):5267-5276.

[27] Yang, H., Zhao, R., Yang, H.Y., Lee, M.H., 2005. Constitutively active FOXO4 inhibits Akt activity, regulates p27 Kip1 stability, and suppresses HER2-mediated tumorigenicity. Oncogene, 24(11):1924-1935.

[28] Zhang, D.W., Li, X.Y., Chen, L.T., 2001. Animal models and pathological changes in gastroesophageal reflux. Chin. J. Pediatr. Surg., 22(2):112-114.

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