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Abstract: The effects of equine chorionic gonadotropin (eCG) on follicular development and ovulation in cyclic guinea Pigs were investigated by histological and immunohistochemical analyses. Three groups of guinea Pigs (n=12) were administrated subcutaneously with saline, 20 or 50 IU of eCG, respectively, on cyclic Day 12 (Day 1=vaginal openings). Ovaries were collected at 4 and 8 d after administration (6 animals per group each time). The eCG administration induced significant and distinct morphological changes in the ovaries, as it promoted the luteinization of granulosa cells, but not follicular development. In addition, proliferating cell nuclear antigen (PCNA) and steroidogenic acute regulatory protein (StAR) were immunolocalized specifically in luteinized follicles. Our experiments together indicate that eCG administration can induce follicular luteinization but not superovulation in guinea Pigs. The eCG in cyclic guinea Pigs functions similar to that of luteinizing hormone (LH), but not follicle-stimulating hormone (FSH).

正常发情周期豚鼠经孕马血清促性腺激素诱导的卵泡黄体化研究

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