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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.5 P.355-367


Osteogenic differentiation of mesenchymal stem cells promoted by overexpression of connective tissue growth factor

Author(s):  Jin-jing WANG, Feng YE, Li-jia CHENG, Yu-jun SHI, Ji BAO, Huai-qiang SUN, Wei WANG, Peng ZHANG, Hong BU

Affiliation(s):  Key Laboratory of Transplant Engineering and Immunology of Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China; more

Corresponding email(s):   hongbu@scu.edu.cn

Key Words:  Mesenchymal stem cells (MSCs), Connective tissue growth factor (CTGF), Osteogenic differentiation, Osteoblasts, Overexpression, Gene modification

Jin-jing WANG, Feng YE, Li-jia CHENG, Yu-jun SHI, Ji BAO, Huai-qiang SUN, Wei WANG, Peng ZHANG, Hong BU. Osteogenic differentiation of mesenchymal stem cells promoted by overexpression of connective tissue growth factor[J]. Journal of Zhejiang University Science B, 2009, 10(5): 355-367.

@article{title="Osteogenic differentiation of mesenchymal stem cells promoted by overexpression of connective tissue growth factor",
author="Jin-jing WANG, Feng YE, Li-jia CHENG, Yu-jun SHI, Ji BAO, Huai-qiang SUN, Wei WANG, Peng ZHANG, Hong BU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Osteogenic differentiation of mesenchymal stem cells promoted by overexpression of connective tissue growth factor
%A Jin-jing WANG
%A Feng YE
%A Li-jia CHENG
%A Yu-jun SHI
%A Huai-qiang SUN
%A Hong BU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 5
%P 355-367
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820252

T1 - Osteogenic differentiation of mesenchymal stem cells promoted by overexpression of connective tissue growth factor
A1 - Jin-jing WANG
A1 - Feng YE
A1 - Li-jia CHENG
A1 - Yu-jun SHI
A1 - Ji BAO
A1 - Huai-qiang SUN
A1 - Wei WANG
A1 - Peng ZHANG
A1 - Hong BU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 5
SP - 355
EP - 367
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820252

Objective: Large segmental bone defect repair remains a clinical and scientific challenge with increasing interest focusing on combining gene transfection with tissue engineering techniques. The aim of this study is to investigate the effect of connective tissue growth factor (CTGF) on the proliferation and osteogenic differentiation of the bone marrow mesenchymal stem cells (MSCs). Methods: A CTGF-expressing plasmid (pCTGF) was constructed and transfected into MSCs. Then expressions of bone morphogenesis-related genes, proliferation rate, alkaline phosphatase activity, and mineralization were examined to evaluate the osteogenic potential of the CTGF gene-modified MSCs. Results: overexpression of CTGF was confirmed in pCTGF-MSCs. pCTGF transfection significantly enhanced the proliferation rates of pCTGF-MSCs (P<0.05). CTGF induced a 7.5-fold increase in cell migration over control (P<0.05). pCTGF transfection enhanced the expression of bone matrix proteins, such as bone sialoprotein, osteocalcin, and collagen type I in MSCs. The levels of alkaline phosphatase (ALP) activities of pCTGF-MSCs at the 1st and 2nd weeks were 4.0- and 3.0-fold higher than those of MSCs cultured in OS-medium, significantly higher than those of mock-MSCs and normal control MSCs (P<0.05). overexpression of CTGF in MSCs enhanced the capability to form mineralized nodules. Conclusion: overexpression of CTGF could improve the osteogenic differentiation ability of MSCs, and the CTGF gene-modified MSCs are potential as novel cell resources of bone tissue engineering.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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