Full Text:   <2168>

CLC number: Q943.2

On-line Access: 2010-11-04

Received: 2010-02-10

Revision Accepted: 2010-04-05

Crosschecked: 2010-10-15

Cited: 8

Clicked: 5021

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.11 P.862-870


Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination

Author(s):  Qiao-yun Li, Hong-bin Niu, Jun Yin, Hong-bo Shao, Ji-shan Niu, Jiang-ping Ren, Yong-chun Li, Xiang Wang

Affiliation(s):  National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002, China, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China, Institute of Life Sciences, Qingdao University of Science and Technology (QUST), Qingdao 266042, China

Corresponding email(s):   xmzxyj@126.com, shaohongbochu@126.com

Key Words:  Phalaris coerulescens thioredoxin gene (PTrx), Aluminum, Transgenic barley, Oxidative stress

Qiao-yun Li, Hong-bin Niu, Jun Yin, Hong-bo Shao, Ji-shan Niu, Jiang-ping Ren, Yong-chun Li, Xiang Wang. Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination[J]. Journal of Zhejiang University Science B, 2010, 11(11): 862-870.

@article{title="Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination",
author="Qiao-yun Li, Hong-bin Niu, Jun Yin, Hong-bo Shao, Ji-shan Niu, Jiang-ping Ren, Yong-chun Li, Xiang Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination
%A Qiao-yun Li
%A Hong-bin Niu
%A Jun Yin
%A Hong-bo Shao
%A Ji-shan Niu
%A Jiang-ping Ren
%A Yong-chun Li
%A Xiang Wang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 11
%P 862-870
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000048

T1 - Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination
A1 - Qiao-yun Li
A1 - Hong-bin Niu
A1 - Jun Yin
A1 - Hong-bo Shao
A1 - Ji-shan Niu
A1 - Jiang-ping Ren
A1 - Yong-chun Li
A1 - Xiang Wang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 11
SP - 862
EP - 870
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000048

A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl3 treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl3 during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Asada, K., 1984. Chloroplasts: formation of active oxygen and its scavenging. Methods Enzymol., 105:422-429.

[2]Balmer, Y., Vensel, W.H., Tanaka, C.K., Hurkman, W.J., Gelhaye, E., Rouhier, N., Jacquot, J.P., Manieri, W., Schürmann, P., Droux, M., et al., 2004. Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria. PNAS, 101(8):2642-2647.

[3]Boscolo, P.R.S., Menossi, M., Jorge, R.A., 2003. Aluminum-induced oxidative stress in maize. Phytochemistry, 62(2):181-189.

[4]Bradford, M.M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2):248-254.

[5]Broin, M., Rey, P., 2003. Potato plants lacking the CDSP32 plastidic thioredoxin exhibit over-oxidation of the BAS1 2-Cys peroxiredoxin and increased lipid peroxidation in thylakoids under photooxidative stress. Plant Physiol., 132(3):1335-1343.

[6]Cakmak, I., Horst, W.J., 1991. Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxides activities in root tips of soybean (Glycine max). Physiol. Plantarum, 83(3):463-468.

[7]Delisle, G., Champoux, M., Houde, M., 2001. Characterization of oxalate oxidase and cell death in Al-sensitive and tolerant wheat roots. Plant Cell Physiol., 42(3):324-333.

[8]Ellman, G.D., 1959. Tissue sulfhydryl groups. Arch. Biochem. Biophys., 82(1):70-77.

[9]Goodwin, S.B., Sutter, T.R., 2009. Microarray analysis of Arabidopsis genome response to aluminum stress. Biol. Plantarum, 53(1):85-99.

[10]Jung, B.G., Lee, K.O., Lee, S.S., Chi, Y.H., Jang, H.H., Kang, S.S., Lee, K., Lim, D., Yoon, S.H., Yun, D.J., et al., 2002. A Chinese cabbage cDNA with high sequence identity to phospholipid hydroperoxide glutathione peroxidases encodes a novel isoform of thioredoxin-dependent peroxidase. J. Biol. Chem., 277(15):12572-12578.

[11]Kochian, L.V., 1995. Cellular mechanisms of aluminum toxicity and resistance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 46(1):237-260.

[12]Laloi, C., Mestres-Ortega, D., Marco, Y., Meyer, Y., Reichheld, J.P., 2004. The Arabidopsis cytosolic thioredoxin h5 gene induction by oxidative stress and its W-box-mediated response to pathogen elicitor. Plant Physiol., 134(3):1006-1016.

[13]Lemaire, S.D., Collin, V., Keryer, E., Issakidis-Bourguet, E., Lavergne, D., 2003. Chlamydomonas reinhardtii: a model organism for the study of the thioredoxin family. Plant Physiol. Biochem., 41(6-7):513-521.

[14]Levine, R.L., Garland, D., Olive, C.N., Amici, A., Climent, I., Lenz, A.G., Ahn, B.W., Shaltiel, S., Stadtman, E.R., 1990. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol., 186:464-478.

[15]Li, X.M., Nield, J., Hayman, D., Langridge, P., 1995. Thioredoxin activity in the C terminus of Phalaris S protein. Plant J., 8(1):133-138.

[16]Li, Y.C., Ren, J.P., Cho, M.J., Zhou, S.M., Kim, Y.B., Guo, H.X., Wong, J.H., Niu, H.B., Kim, H.K., Morigasaki, S., et al., 2009. The level of expression of thioredoxin is linked to fundamental properties and applications of wheat seeds. Mol. Plant, 2(3):430-441.

[17]Livingstone, D.R., Lips, F., Garcia Martinez, P., Pipe, P.K., 1992. Antioxidant enzymes in the digestive gland of the common mussel Mytilus edulis. Mar. Biol., 112(2):265-276.

[18]Maron, L.G., Kirst, M., Mao, C., Milner, M.J., Menossi, M., Kochian, L.V., 2008. Transcriptional profiling of aluminum toxicity and tolerance responses in maize roots. New Phytol., 179(1):116-128.

[19]Meyer, Y., Verdoucq, L., Vignols, F., 1999. Plant thioredoxins and glutaredoxins: identity and putative roles. Trends Plant Sci., 4(10):388-394.

[20]Mittler, R., 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci., 7(9):405-410.

[21]Pan, J.W., Zhu, M.Y., Chen, H., 2001. Aluminum-induced cell death in root-tip cells of barley. Environ. Exp. Bot., 46(1):71-79.

[22]Rey, P., Cuiné, S., Eymery, F., Garin, J., Court, M., Jacquot, J.P., Rouhier, N., Broin, M., 2005. Analysis of the proteins targeted by CDSP32, a plastidic thioredoxin participating in oxidative stress responses. Plant J., 41(1):31-42.

[23]Richards, K.D., Schott, E.J., Sharma, Y.K., Davis, K.R., Gardner, R.C., 1998. Aluminum induces oxidative stress genes in Arabidopsis thaliana. Plant Physiol., 116(1):409-418.

[24]Sato, K., Shin-I, T., Seki, M., Shinozaki, K., Yoshida, H., Takeda, K., Yamazaki, Y., Conte, M., Kohara, Y., 2009. Development of 5006 full-length CDNAs in barley: a tool for accessing cereal genomics resources. DNA Res., 16(2):81-89.

[25]Schaedle, M., Bassham, J.A., 1977. Chloroplast glutathione reductase. Plant Physiol., 59(5):1011-1012.

[26]Schenk, H., Klein, M., Erdbrugger, W., Droge, W., Schulze-Osthoff, K., 1994. Distinct effects of thioredoxin and antioxidants on the activation of transcription factors NF-κB and AP-1. PNAS, 9l(5):1672-1676.

[27]Shao, H.B., Jiang, S.Y., Li, F.M., Chu, L.Y., Shao, M.A., Li, F., 2007a. Some advances in plant stress physiology and their implications in the systems biology era. Colloids Surf. B Biointerfaces, 54(1):33-36.

[28]Shao, H.B., Chu, L.Y., Lu, Z.H., Kang, C.M., 2007b. Primary antioxidant free radical scavenging and redox signaling pathways in higher plant cells. Int. J. Biol. Sci., 4(1):8-14.

[29]Shao, H.B., Chu, L.Y., Shao, M.A., 2008. Calcium as a versatile plant signal transducer under soil water stress. Bioessays, 30(7):634-641.

[30]Shao, H.B., Jaleel, C.A., Manivannan, P., Panneerselvam, R., Shao, M.A., 2009. Understanding water deficit stress-induced changes in the basic metabolism of higher plants—biotechnologically and sustainably improving agriculture and the ecoenvironment in arid regions of the globe. Crit. Rev. Biotechnol., 29(2):131-151.

[31]Tamás, L., Budíková, S., Šimonovičová, M., Huttova, J., Siroka, B., Mistrik, I., 2006. Rapid and simple method for Al-toxicity analysis in emerging barley roots during germination. Biol. Plantarum, 50(1):87-93.

[32]Tsukamoto, S., Morita, S., Hirano, E., Yokoi, H., Masumura, T., Tanaka, K., 2005. A novel cis-element that is responsive to oxidative stress regulates three antioxidant defense genes in rice. Plant Physiol., 137(1):317-327.

[33]Vieira Dos Santos, C., Rey, P., 2006. Plant thioredoxins are key actors in the oxidative stress response. Trends Plant Sci., 11(7):329-334.

[34]Wang, G.L., Fang, H.J., 1998. Principle and Technique of Plant Genetic Engineering. Science Press, Beijing (in Chinese).

[35]Wong, J.H., Cai, N., Balmer, Y., Tanaka, C.K., Vensel,W.H., Hurkman, W.J., Buchanan, B.B., 2004. Thioredoxin targets of developing wheat seeds identified by complementary proteomic approaches. Phytochemistry, 65(11):1629-1640.

[36]Yamamoto, Y., Kobayashi, Y., Matsumoto, H., 2001. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol., 125(1):199-208.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE