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On-line Access: 2010-11-04

Received: 2010-02-10

Revision Accepted: 2010-04-05

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.11 P.862-870

http://doi.org/10.1631/jzus.B1000048


Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination


Author(s):  Qiao-yun Li, Hong-bin Niu, Jun Yin, Hong-bo Shao, Ji-shan Niu, Jiang-ping Ren, Yong-chun Li, Xiang Wang

Affiliation(s):  National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002, China, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China, Institute of Life Sciences, Qingdao University of Science and Technology (QUST), Qingdao 266042, China

Corresponding email(s):   xmzxyj@126.com, shaohongbochu@126.com

Key Words:  Phalaris coerulescens thioredoxin gene (PTrx), Aluminum, Transgenic barley, Oxidative stress


Qiao-yun Li, Hong-bin Niu, Jun Yin, Hong-bo Shao, Ji-shan Niu, Jiang-ping Ren, Yong-chun Li, Xiang Wang. Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination[J]. Journal of Zhejiang University Science B, 2010, 11(11): 862-870.

@article{title="Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination",
author="Qiao-yun Li, Hong-bin Niu, Jun Yin, Hong-bo Shao, Ji-shan Niu, Jiang-ping Ren, Yong-chun Li, Xiang Wang",
journal="Journal of Zhejiang University Science B",
volume="11",
number="11",
pages="862-870",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000048"
}

%0 Journal Article
%T Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination
%A Qiao-yun Li
%A Hong-bin Niu
%A Jun Yin
%A Hong-bo Shao
%A Ji-shan Niu
%A Jiang-ping Ren
%A Yong-chun Li
%A Xiang Wang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 11
%P 862-870
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000048

TY - JOUR
T1 - Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination
A1 - Qiao-yun Li
A1 - Hong-bin Niu
A1 - Jun Yin
A1 - Hong-bo Shao
A1 - Ji-shan Niu
A1 - Jiang-ping Ren
A1 - Yong-chun Li
A1 - Xiang Wang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 11
SP - 862
EP - 870
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000048


Abstract: 
A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl3 treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl3 during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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