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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.10 P.801-805

http://doi.org/10.1631/jzus.B1000051


Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts


Author(s):  Yuriy G. Shckorbatov, Vladimir N. Pasiuga, Elena I. Goncharuk, Tatiana Ph. Petrenko, Valentin A. Grabina, Nicolay N. Kolchigin, Dmitry D. Ivanchenko, Victor N. Bykov, Oleksandr M. Dumin

Affiliation(s):  Kharkiv National University, pl. Svobody 4, Kharkiv 61077, Ukraine; more

Corresponding email(s):   Yury.G.Shckorbatov@univer.kharkov.ua, goncharuk_elena@rambler.ru

Key Words:  Human cells, Chromatin, Heterochromatin, Electromagnetic field, Elliptical polarization


Yuriy G. Shckorbatov, Vladimir N. Pasiuga, Elena I. Goncharuk, Tatiana Ph. Petrenko, Valentin A. Grabina, Nicolay N. Kolchigin, Dmitry D. Ivanchenko, Victor N. Bykov, Oleksandr M. Dumin. Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts[J]. Journal of Zhejiang University Science B, 2010, 11(10): 801-805.

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author="Yuriy G. Shckorbatov, Vladimir N. Pasiuga, Elena I. Goncharuk, Tatiana Ph. Petrenko, Valentin A. Grabina, Nicolay N. Kolchigin, Dmitry D. Ivanchenko, Victor N. Bykov, Oleksandr M. Dumin",
journal="Journal of Zhejiang University Science B",
volume="11",
number="10",
pages="801-805",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000051"
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%0 Journal Article
%T Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts
%A Yuriy G. Shckorbatov
%A Vladimir N. Pasiuga
%A Elena I. Goncharuk
%A Tatiana Ph. Petrenko
%A Valentin A. Grabina
%A Nicolay N. Kolchigin
%A Dmitry D. Ivanchenko
%A Victor N. Bykov
%A Oleksandr M. Dumin
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 10
%P 801-805
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000051

TY - JOUR
T1 - Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts
A1 - Yuriy G. Shckorbatov
A1 - Vladimir N. Pasiuga
A1 - Elena I. Goncharuk
A1 - Tatiana Ph. Petrenko
A1 - Valentin A. Grabina
A1 - Nicolay N. Kolchigin
A1 - Dmitry D. Ivanchenko
A1 - Victor N. Bykov
A1 - Oleksandr M. Dumin
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 10
SP - 801
EP - 805
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000051


Abstract: 
To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm2, exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm2 produced no effect, and irradiation at the intensities of 10 and 100 µW/cm2 induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.

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