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On-line Access: 2011-03-09

Received: 2010-05-03

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Crosschecked: 2011-01-25

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.3 P.219-225


Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita

Author(s):  Xiao-ning Yan, Richard A. Sikora, Jing-wu Zheng

Affiliation(s):  Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China, Soil-Ecosystem Phytopathology and Nematology, Institute for Resource Conservation and Plant Science (INRES), Department of Plant Health, University of Bonn, Bonn 53115, Germany, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Corresponding email(s):   jwzheng@zju.edu.cn

Key Words:  Cucumber endophytic fungi, Meloidogyne incognita, Control efficacy, Colonization, In vitro activity

Xiao-ning Yan, Richard A. Sikora, Jing-wu Zheng. Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita[J]. Journal of Zhejiang University Science B, 2011, 12(3): 219-225.

@article{title="Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita",
author="Xiao-ning Yan, Richard A. Sikora, Jing-wu Zheng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita
%A Xiao-ning Yan
%A Richard A. Sikora
%A Jing-wu Zheng
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 3
%P 219-225
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000165

T1 - Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita
A1 - Xiao-ning Yan
A1 - Richard A. Sikora
A1 - Jing-wu Zheng
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 3
SP - 219
EP - 225
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000165

Seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control. Endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against Meloidogyne incognita. Among the 294 isolates screened, 23 significantly reduced galls formed by M. incognita in greenhouse test. The 10 most effective isolates were Fusarium (5), Trichoderma (1), Chaetomium (1), Acremonium (1), Paecilomyces (1), and Phyllosticta (1). Their control efficacies were repeatedly tested and their colonizations as well as in vitro activity against M. incognita were studied. They reduced the number of galls by 24.0%–58.4% in the first screening and 15.6%–44.3% in the repeated test, respectively. Phyllosticta Ph511 and Chaetomium Ch1001 had high colonizations on both the roots and the aboveground parts of cucumber seedlings. Fusarium isolates had colonization preference on the roots, their root colonizations ranging from 20.1% to 47.3% of the total root area. Trichoderma Tr882, Paecilomyces Pa972, and Acremonium Ac985 had low colonizations on both the roots and the aboveground parts. Acremonium Ac985, Chaetomium Ch1001, Paecilomyces Pa972, and Phyllosticta Ph511 produced compounds affecting motility of the second stage juveniles of M. incognita. Based on these results, Chaetomium Ch1001 was considered to have the highest potential as a seed treatment agent for M. incognita biocontrol.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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