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CLC number: R733.7

On-line Access: 2010-09-08

Received: 2010-05-31

Revision Accepted: 2010-06-25

Crosschecked: 2010-08-02

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.9 P.647-653

http://doi.org/10.1631/jzus.B1000198


Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients


Author(s):  Yan-qin Huang, Ying Yuan, Wei-ting Ge, Han-guang Hu, Su-zhan Zhang, Shu Zheng

Affiliation(s):  Key Laboratory of Cancer Prevention and Intervention of Ministry of Education, Key Laboratory of Molecular Biology in Medical Science of Zhejiang Province, Cancer Institute, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   zhengshu@zju.edu.cn

Key Words:  Microsatellite instability, Colorectal cancer, Gastric cancer, Mutation, DNA methylation


Yan-qin Huang, Ying Yuan, Wei-ting Ge, Han-guang Hu, Su-zhan Zhang, Shu Zheng. Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients[J]. Journal of Zhejiang University Science B, 2010, 11(9): 647-653.

@article{title="Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients",
author="Yan-qin Huang, Ying Yuan, Wei-ting Ge, Han-guang Hu, Su-zhan Zhang, Shu Zheng",
journal="Journal of Zhejiang University Science B",
volume="11",
number="9",
pages="647-653",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000198"
}

%0 Journal Article
%T Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients
%A Yan-qin Huang
%A Ying Yuan
%A Wei-ting Ge
%A Han-guang Hu
%A Su-zhan Zhang
%A Shu Zheng
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 9
%P 647-653
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000198

TY - JOUR
T1 - Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients
A1 - Yan-qin Huang
A1 - Ying Yuan
A1 - Wei-ting Ge
A1 - Han-guang Hu
A1 - Su-zhan Zhang
A1 - Shu Zheng
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 9
SP - 647
EP - 653
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000198


Abstract: 
Objective: The purpose of this study was to determine the unique and universal features of microsatellite instability-high (MSI-H) colorectal cancer (CRC) and MSI-H gastric cancer (GC) in the Chinese population. Methods: A new panel of mononucleotide MSI markers, BAT25, BAT26, NR21, NR24, and MONO-27, was used to define MSI status in 303 CRC and 288 GC subjects. Clinicopathological features of both types of MSI-H tumors were analyzed. Methylation analysis in the hMLH1 promoter region by methylation specific polymerase chain reaction (PCR) and mutation detection of hMSH2/hMLH1 genes by denaturing high-performance liquid chromatography (DHPLC) were carried out simultaneously. Results: MSI-H CRCs and MSI-H GCs account for 11.9% and 8.0% of unselected sporadic CRCs and GCs, respectively. MSI-H CRCs are strongly characterized by early onset, right-side location, low differentiation, mucinous tumor, less infiltration, less lymphatic metastasis, and more often familial tumor. MSI-H GCs only showed site preference for the antrum and less lymphatic metastasis. Genetic and epigenetic analyses were positive in 6/36 MSI-H CRCs and 0/23 MSI-H GCs with pathological mutation in major mismatch repair genes, and in 7/36 MSI-H CRCs and 18/23 MSI-H GCs with methylated hMLH1 promoter (P<0.01), respectively. Conclusions: Although there are many differences in the genetic basis and clinicopathological features between MSI-H CRC and MSI-H GC, when compared with their microsatellite stable (MSS) counterparts, site preference and lymphatic metastasis are features common to both types of MSI-H tumors.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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