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CLC number: R36

On-line Access: 2017-09-05

Received: 2016-07-09

Revision Accepted: 2016-10-26

Crosschecked: 2017-08-16

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Yi-ping Tian


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.9 P.747-756


Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer

Author(s):  Yi-ping Tian, Ai-fen Lin, Mei-fu Gan, Hao Wang, Dan Yu, Chong Lai, Dan-dan Zhang, Yi-min Zhu, Mao-de Lai

Affiliation(s):  Department of Pathology, School of Medicine, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   zhuym@zju.edu.cn, lmd@zju.edu.cn

Key Words:  5-Hydroxymethylcytosine (5hmC), 5-Methylcytosine (5mC), Prognosis, Colorectal cancer

Yi-ping Tian, Ai-fen Lin, Mei-fu Gan, Hao Wang, Dan Yu, Chong Lai, Dan-dan Zhang, Yi-min Zhu, Mao-de Lai. Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer[J]. Journal of Zhejiang University Science B, 2017, 18(9): 747-756.

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author="Yi-ping Tian, Ai-fen Lin, Mei-fu Gan, Hao Wang, Dan Yu, Chong Lai, Dan-dan Zhang, Yi-min Zhu, Mao-de Lai",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer
%A Yi-ping Tian
%A Ai-fen Lin
%A Mei-fu Gan
%A Hao Wang
%A Dan Yu
%A Chong Lai
%A Dan-dan Zhang
%A Yi-min Zhu
%A Mao-de Lai
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 9
%P 747-756
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600314

T1 - Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer
A1 - Yi-ping Tian
A1 - Ai-fen Lin
A1 - Mei-fu Gan
A1 - Hao Wang
A1 - Dan Yu
A1 - Chong Lai
A1 - Dan-dan Zhang
A1 - Yi-min Zhu
A1 - Mao-de Lai
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 9
SP - 747
EP - 756
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600314

Aberrant DNA methylation has raised widespread attention in tumorigenesis. In this study, we aimed to investigate the changes of global DNA methylation and hydroxymethylation from normal to tumor tissues in colorectal cancer (CRC) and their association with the prognosis. The levels of genomic 5-Hydroxymethylcytosine (5hmC) and 5-Methylcytosine (5mC) in cancerous tissues were significantly lower than those in corresponding adjacent normal tissues. The genomic levels of 5mC were significantly positively correlated with 5hmC in normal and cancerous tissues (all P<0.05). The ratio of 5mC in cancerous tissues to matched normal tissues (C/N-5mC) was also significantly positively correlated with the ratio of 5hmC in cancerous tissues to matched normal tissues (C/N-5hmC) (P=0.01). The 5mC levels and C/N-5mC ratios decreased with age (all P<0.05). Higher 5mC and 5hmC levels were found in rectal than in colon tissues (all P<0.05). High levels of 5mC in cancerous tissues and high C/N-5hmC ratios were each associated with lymph node metastasis (all P<0.05). Survival analysis indicated that the C/N-5mC ratio (P=0.04) is an independent protective factor for overall survival. The data showed that patients with a combination of high C/N-5hmC and low C/N-5mC ratios tended to have a worse prognosis (P<0.01). Our findings showed that the C/N-5mC ratio may be an independent prognostic factor for CRC outcome. Patients with both a high C/N-5hmC ratio and a low C/N-5mC ratio exhibited the worst survival, suggesting that 5mC and 5hmC can be used as critical markers in tumorigenesis and prognosis estimation.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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