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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.11 P.915-922

http://doi.org/10.1631/jzus.B1000315


Antioxidants in aqueous extract of Myristica fragrans (Houtt.) suppress mitosis and cyclophosphamide-induced chromosomal aberrations in Allium cepa L. cells


Author(s):  Akeem Akinboro, Kamaruzaman Bin Mohamed, Mohd Zaini Asmawi, Shaida Fariza Sulaiman, Othman Ahmad Sofiman

Affiliation(s):  School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia

Corresponding email(s):   mkamar_usm@yahoo.com

Key Words:  Allium cepa, Antioxidants, Chromosomal aberration, Cyclophosphamide, Mitotic index


Akeem Akinboro, Kamaruzaman Bin Mohamed, Mohd Zaini Asmawi, Shaida Fariza Sulaiman, Othman Ahmad Sofiman. Antioxidants in aqueous extract of Myristica fragrans (Houtt.) suppress mitosis and cyclophosphamide-induced chromosomal aberrations in Allium cepa L. cells[J]. Journal of Zhejiang University Science B, 2011, 12(11): 915-922.

@article{title="Antioxidants in aqueous extract of Myristica fragrans (Houtt.) suppress mitosis and cyclophosphamide-induced chromosomal aberrations in Allium cepa L. cells",
author="Akeem Akinboro, Kamaruzaman Bin Mohamed, Mohd Zaini Asmawi, Shaida Fariza Sulaiman, Othman Ahmad Sofiman",
journal="Journal of Zhejiang University Science B",
volume="12",
number="11",
pages="915-922",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000315"
}

%0 Journal Article
%T Antioxidants in aqueous extract of Myristica fragrans (Houtt.) suppress mitosis and cyclophosphamide-induced chromosomal aberrations in Allium cepa L. cells
%A Akeem Akinboro
%A Kamaruzaman Bin Mohamed
%A Mohd Zaini Asmawi
%A Shaida Fariza Sulaiman
%A Othman Ahmad Sofiman
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 11
%P 915-922
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000315

TY - JOUR
T1 - Antioxidants in aqueous extract of Myristica fragrans (Houtt.) suppress mitosis and cyclophosphamide-induced chromosomal aberrations in Allium cepa L. cells
A1 - Akeem Akinboro
A1 - Kamaruzaman Bin Mohamed
A1 - Mohd Zaini Asmawi
A1 - Shaida Fariza Sulaiman
A1 - Othman Ahmad Sofiman
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 11
SP - 915
EP - 922
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000315


Abstract: 
In this study, freeze-dried water extract from the leaves of Myristica fragrans (Houtt.) was tested for mutagenic and antimutagenic potentials using the Allium cepa assay. Freeze-dried water extract alone and its combination with cyclophosphamide (CP) (50 mg/kg) were separately dissolved in tap water at 500, 1000, 2000, and 4000 mg/kg. Onions (A. cepa) were suspended in the solutions and controls for 48 h in the dark. Root tips were prepared for microscopic evaluation. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radicals’ scavenging power of the extract was tested using butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as standards. Water extract of Myristica fragrans scavenged free radicals better than BHA, but worse than BHT. The extract alone, as well as in combination with CP suppressed cell division, and induced chromosomal aberrations that were insignificantly different from the negative control (P≤0.05). However, cytotoxic and mutagenic actions of CP were considerably suppressed. The observed effects on cell division and chromosomes of A. cepa may be principally connected to the antioxidant properties of the extract. The obtained results suggest mitodepressive and antimutagenic potentials of water extract of the leaves of M. fragrans as desirable properties of a promising anticancer agent.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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