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On-line Access: 2011-12-30

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.1 P.20-28


Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro

Author(s):  Yu-li Huang, Ruo-feng Qiu, Wei-yi Mai, Jian Kuang, Xiao-yan Cai, Yu-gang Dong, Yun-zhao Hu, Yuan-bin Song, An-ping Cai, Zhi-gao Jiang

Affiliation(s):  Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China, Department of Cardiology, the First People's Hospital of Shunde, Foshan 528300, China

Corresponding email(s):   wymai@hotmail.com

Key Words:  Mesenchymal stem cells (MSCs), Proliferation, Differentiation, Insulin-like growth factor-1 (IGF-1), CXC chemokine receptor 4 (CXCR4), Migration

Yu-li Huang, Ruo-feng Qiu, Wei-yi Mai, Jian Kuang, Xiao-yan Cai, Yu-gang Dong, Yun-zhao Hu, Yuan-bin Song, An-ping Cai, Zhi-gao Jiang. Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro[J]. Journal of Zhejiang University Science B, 2012, 13(1): 20-28.

@article{title="Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro",
author="Yu-li Huang, Ruo-feng Qiu, Wei-yi Mai, Jian Kuang, Xiao-yan Cai, Yu-gang Dong, Yun-zhao Hu, Yuan-bin Song, An-ping Cai, Zhi-gao Jiang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro
%A Yu-li Huang
%A Ruo-feng Qiu
%A Wei-yi Mai
%A Jian Kuang
%A Xiao-yan Cai
%A Yu-gang Dong
%A Yun-zhao Hu
%A Yuan-bin Song
%A An-ping Cai
%A Zhi-gao Jiang
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 1
%P 20-28
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100117

T1 - Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro
A1 - Yu-li Huang
A1 - Ruo-feng Qiu
A1 - Wei-yi Mai
A1 - Jian Kuang
A1 - Xiao-yan Cai
A1 - Yu-gang Dong
A1 - Yun-zhao Hu
A1 - Yuan-bin Song
A1 - An-ping Cai
A1 - Zhi-gao Jiang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 1
SP - 20
EP - 28
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100117

Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5–20 ng/ml. proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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