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CLC number: Q939.9; Q969.91

On-line Access: 2012-04-06

Received: 2011-05-15

Revision Accepted: 2011-09-29

Crosschecked: 2012-02-29

Cited: 5

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.4 P.261-266

http://doi.org/10.1631/jzus.B1100155


Acaricidal activities of whole cell suspension, cell-free supernatant, and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.)


Author(s):  Prapassorn Bussaman, Chirayu Sa-Uth, Paweena Rattanasena, Angsumarn Chandrapatya

Affiliation(s):  Biocontrol Research Unit, Department of Biotechnology, Mahasarakham University, Maha Sarakham 44150, Thailand; more

Corresponding email(s):   agramc@ku.ac.th

Key Words:  Xenorhabdus, Luciaphorus, Whole cell suspension, Cell-free supernatant, Crude cell extract, Mushroom mite


Prapassorn Bussaman, Chirayu Sa-Uth, Paweena Rattanasena, Angsumarn Chandrapatya. Acaricidal activities of whole cell suspension, cell-free supernatant, and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.)[J]. Journal of Zhejiang University Science B, 2012, 13(4): 261-266.

@article{title="Acaricidal activities of whole cell suspension, cell-free supernatant, and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.)",
author="Prapassorn Bussaman, Chirayu Sa-Uth, Paweena Rattanasena, Angsumarn Chandrapatya",
journal="Journal of Zhejiang University Science B",
volume="13",
number="4",
pages="261-266",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100155"
}

%0 Journal Article
%T Acaricidal activities of whole cell suspension, cell-free supernatant, and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.)
%A Prapassorn Bussaman
%A Chirayu Sa-Uth
%A Paweena Rattanasena
%A Angsumarn Chandrapatya
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 4
%P 261-266
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100155

TY - JOUR
T1 - Acaricidal activities of whole cell suspension, cell-free supernatant, and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.)
A1 - Prapassorn Bussaman
A1 - Chirayu Sa-Uth
A1 - Paweena Rattanasena
A1 - Angsumarn Chandrapatya
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 4
SP - 261
EP - 266
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100155


Abstract: 
Xenorhabdus bacterium has been used as a biological control agent against Luciaphorus sp., a mushroom mite endemic in Thailand. To develop an effective formulation of Xenorhabdus stokiae, treatments using different parts of X. stokiae isolate PB09 culture, including whole cell suspension, cell-free supernatant, and crude cell extract, were performed. The results show that different parts of X. stokiae isolate PB09 culture could induce variable effects on mite mortality and fecundity. Application with cell-free supernatant of X. stokiae culture resulted in both the highest mite mortality rate [(89.00±3.60)%] and the lowest mite fecundity [(41.33±23.69) eggs/gravid female]. whole cell suspension of X. stokiae isolate PB09 culture was found to be slightly less effective than its cell-free supernatant, suggesting that X. stokiae was more likely to release its metabolites with acaricidal activities to the surrounding culture media. crude cell extract of X. stokiae was not effective against mites. cell-free supernatant of X. stokiae isolate PB09 was the most effective biological control agent and it could be conveniently used in future formulations instead of live bacteria.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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