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CLC number: R737.1

On-line Access: 2012-05-04

Received: 2011-12-08

Revision Accepted: 2012-04-11

Crosschecked: 2012-04-16

Cited: 9

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.5 P.335-341

http://doi.org/10.1631/jzus.B1100366


Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells


Author(s):  Guo-qing Ding, Yan-lan Yu, Zhou-jun Shen, Xie-lai Zhou, Shan-wen Chen, Guo-dong Liao, Yue Zhang

Affiliation(s):  Department of Urology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; more

Corresponding email(s):   yanlanyu@gmail.com

Key Words:  Bacillus Calmette-Gué, rin (BCG) vaccine, Bladder neoplasms, Gene recombination, Interferon-alpha 2b


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Guo-qing Ding, Yan-lan Yu, Zhou-jun Shen, Xie-lai Zhou, Shan-wen Chen, Guo-dong Liao, Yue Zhang. Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells[J]. Journal of Zhejiang University Science B, 2012, 13(5): 335-341.

@article{title="Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells",
author="Guo-qing Ding, Yan-lan Yu, Zhou-jun Shen, Xie-lai Zhou, Shan-wen Chen, Guo-dong Liao, Yue Zhang",
journal="Journal of Zhejiang University Science B",
volume="13",
number="5",
pages="335-341",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100366"
}

%0 Journal Article
%T Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells
%A Guo-qing Ding
%A Yan-lan Yu
%A Zhou-jun Shen
%A Xie-lai Zhou
%A Shan-wen Chen
%A Guo-dong Liao
%A Yue Zhang
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 5
%P 335-341
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100366

TY - JOUR
T1 - Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells
A1 - Guo-qing Ding
A1 - Yan-lan Yu
A1 - Zhou-jun Shen
A1 - Xie-lai Zhou
A1 - Shan-wen Chen
A1 - Guo-dong Liao
A1 - Yue Zhang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 5
SP - 335
EP - 341
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100366


Abstract: 
Objective: Our objective was to construct a recombinant bacillus Calmette-Gué;rin vaccine (rBCG) that secretes human interferon-alpha 2b (IFNα-2b) and to study its immunogenicity and in vitro antitumor activity against human bladder cancer cell lines T24 and T5637. Methods: The signal sequence BCG Ag85B and the gene IFNα-2b were amplified from the genome of BCG and human peripheral blood, respectively, by polymerase chain reaction (PCR). The two genes were cloned in Escherichia coli-BCG shuttle-vector pMV261 to obtain a new recombinant plasmid pMV261-Ag85B-IFNα-2b. BCG was transformed with the recombinant plasmid by electroporation and designated rBCG-IFNα-2b. Mononuclear cells were isolated from human peripheral blood (PBMCs) and stimulated with rBCG-IFNα-2b or wild type BCG for 3 d, and then cultured with human bladder cancer cell lines T24 and T5637. Their cytotoxicities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: BCG was successfully transformed with the recombinant plasmid pMV261-Ag85B-IFNα-2b by electroporation and the recombinant BCG (rBCG-IFNα-2b) was capable of synthesizing and secreting cytokine IFNα-2b. PBMC proliferation was enhanced significantly by rBCG-IFNα-2b, and the cytotoxicity of PBMCs stimulated by rBCG-IFNα-2b to T24 and T5627 was significantly stronger in comparison to wild type BCG. Conclusions: A recombinant BCG, secreting human IFNα-2b (rBCG-IFNα-2b), was constructed successfully and was superior to control wild type BCG in inducing immune responses and enhancing cytotoxicity to human bladder cancer cell lines T24 and T5637. This suggests that rBCG-IFNα-2b could be a promising agent for bladder cancer patients in terms of possible reductions in both clinical dosage and side effects of BCG immunotherapy.

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