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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.10 P.769-782

http://doi.org/10.1631/jzus.B1200013


Abundance and composition of ammonia-oxidizing bacteria and archaea in different types of soil in the Yangtze River estuary


Author(s):  Xiao-ran Li, Yi-ping Xiao, Wen-wei Ren, Zeng-fu Liu, Jin-huan Shi, Zhe-xue Quan

Affiliation(s):  Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China; more

Corresponding email(s):   quanzx@fudan.edu.cn

Key Words:  Wetland, Nitrification, Ammonia-oxidizing microorganisms, Abundance, Composition


Xiao-ran Li, Yi-ping Xiao, Wen-wei Ren, Zeng-fu Liu, Jin-huan Shi, Zhe-xue Quan. Abundance and composition of ammonia-oxidizing bacteria and archaea in different types of soil in the Yangtze River estuary[J]. Journal of Zhejiang University Science B, 2012, 13(10): 769-782.

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author="Xiao-ran Li, Yi-ping Xiao, Wen-wei Ren, Zeng-fu Liu, Jin-huan Shi, Zhe-xue Quan",
journal="Journal of Zhejiang University Science B",
volume="13",
number="10",
pages="769-782",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200013"
}

%0 Journal Article
%T Abundance and composition of ammonia-oxidizing bacteria and archaea in different types of soil in the Yangtze River estuary
%A Xiao-ran Li
%A Yi-ping Xiao
%A Wen-wei Ren
%A Zeng-fu Liu
%A Jin-huan Shi
%A Zhe-xue Quan
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 10
%P 769-782
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200013

TY - JOUR
T1 - Abundance and composition of ammonia-oxidizing bacteria and archaea in different types of soil in the Yangtze River estuary
A1 - Xiao-ran Li
A1 - Yi-ping Xiao
A1 - Wen-wei Ren
A1 - Zeng-fu Liu
A1 - Jin-huan Shi
A1 - Zhe-xue Quan
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 10
SP - 769
EP - 782
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200013


Abstract: 
Tidal flats are soil resources of great significance. nitrification plays a central role in the nitrogen cycle and is often a critical first step in nitrogen removal from estuarine and coastal environments. We determined the abundance as well as composition of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in different soils during land reclamation process. The abundance of AOA was higher than that of AOB in farm land and wild land while AOA was not detected in tidal flats using real-time polymerase chain reaction (PCR). The different abundances of AOB and AOA were negatively correlated with the salinity. The diversities of AOB and AOA were also investigated using clone libraries by amplification of amoA gene. Among AOB, nearly all sequences belonged to the Nitrosomonas lineage in the initial land reclamation process, i.e., tidal flats, while both Nitrosomonas and Nitrosospira lineages were detected in later and transition phases of land reclamation process, farm land and wild land. The ratio of the numbers of sequences of Nitrosomonas and Nitrosospira lineages was positively correlated with the salinity and the net nitrification rate. As for AOA, there was no obvious correlation with the changes in the physicochemical properties of the soil. This study suggests that AOB may be more import than AOA with respect to influencing the different land reclamation process stages.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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