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CLC number: Q815

On-line Access: 2013-01-31

Received: 2012-03-06

Revision Accepted: 2012-07-29

Crosschecked: 2013-01-03

Cited: 5

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.2 P.132-143


Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

Author(s):  Xiao-yan Zhang, Yong Peng, Zhong-rui Su, Qi-he Chen, Hui Ruan, Guo-qing He

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   gqhe@zju.edu.cn

Key Words:  Phytosterol, Mycobacterium neoaurum ZJUVN-08, Androstenedione, Hydroxypropyl-β, -cyclodextrin

Xiao-yan Zhang, Yong Peng, Zhong-rui Su, Qi-he Chen, Hui Ruan, Guo-qing He. Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08[J]. Journal of Zhejiang University Science B, 2013, 14(2): 132-143.

@article{title="Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08",
author="Xiao-yan Zhang, Yong Peng, Zhong-rui Su, Qi-he Chen, Hui Ruan, Guo-qing He",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08
%A Xiao-yan Zhang
%A Yong Peng
%A Zhong-rui Su
%A Qi-he Chen
%A Hui Ruan
%A Guo-qing He
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 2
%P 132-143
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200067

T1 - Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08
A1 - Xiao-yan Zhang
A1 - Yong Peng
A1 - Zhong-rui Su
A1 - Qi-he Chen
A1 - Hui Ruan
A1 - Guo-qing He
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 2
SP - 132
EP - 143
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200067

Biotransformation of phytosterol (PS) by a newly isolated mutant Mycobacterium neoaurum ZJUVN-08 to produce androstenedione has been investigated in this paper. The parameters of the biotransformation process were optimized using fractional factorial design and response surface methodology. androstenedione was the sole product in the fermentation broth catalyzed by the mutant M. neoaurum ZJUVN-08 strain. Results showed that molar ratio of hydroxypropyl-β;-cyclodextrin (HP-β-CD) to PS and substrate concentrations were the two most significant factors affecting androstenedione production. By analyzing the statistical model of three-dimensional surface plot, the optimal process conditions were observed at 0.1 g/L inducer, pH 7.0, molar ratio of HP-β-CD to PS 1.92:1, 8.98 g/L PS, and at 120 h of incubation time. Under these conditions, the maximum androstenedione yield was 5.96 g/L and nearly the same with the non-optimized (5.99 g/L), while the maximum PS conversion rate was 94.69% which increased by 10.66% compared with the non-optimized (84.03%). The predicted optimum conditions from the mathematical model were in agreement with the verification experimental results. It is considered that response surface methodology was a powerful and efficient method to optimize the parameters of PS biotransformation process.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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