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CLC number: S635.9

On-line Access: 2013-01-31

Received: 2012-03-26

Revision Accepted: 2012-07-02

Crosschecked: 2013-01-14

Cited: 22

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.2 P.124-131

http://doi.org/10.1631/jzus.B1200096


Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts


Author(s):  Rong-fang Guo, Gao-feng Yuan, Qiao-mei Wang

Affiliation(s):  Department of Horticulture, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   qmwang@zju.edu.cn

Key Words:  Glucosinolates, Brassica oleracea, Sulforaphane, Myrosinase, NaCl


Rong-fang Guo, Gao-feng Yuan, Qiao-mei Wang. Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts[J]. Journal of Zhejiang University Science B, 2013, 14(2): 124-131.

@article{title="Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts",
author="Rong-fang Guo, Gao-feng Yuan, Qiao-mei Wang",
journal="Journal of Zhejiang University Science B",
volume="14",
number="2",
pages="124-131",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200096"
}

%0 Journal Article
%T Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts
%A Rong-fang Guo
%A Gao-feng Yuan
%A Qiao-mei Wang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 2
%P 124-131
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200096

TY - JOUR
T1 - Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts
A1 - Rong-fang Guo
A1 - Gao-feng Yuan
A1 - Qiao-mei Wang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 2
SP - 124
EP - 131
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200096


Abstract: 
To understand the regulation mechanism of naCl on glucosinolate metabolism in broccoli sprouts, the germination rate, fresh weight, contents of glucosinolates and sulforaphane, as well as myrosinase activity of broccoli sprouts germinated under 0, 20, 40, 60, 80, and 100 mmol/L of naCl were investigated in our experiment. The results showed that glucoerucin, glucobrassicin, and 4-hydroxy glucobrassicin in 7-d-old broccoli sprouts were significantly enhanced and the activity of myrosinase was inhibited by 100 mmol/L of naCl. However, the total glucosinolate content in 7-d-old broccoli sprouts was markedly decreased although the fresh weight was significantly increased after treatment with naCl at relatively low concentrations (20, 40, and 60 mmol/L). naCl treatment at the concentration of 60 mmol/L for 5 d maintained higher biomass and comparatively higher content of glucosinolates in sprouts of broccoli with decreased myrosinase activity. A relatively high level of naCl treatment (100 mmol/L) significantly increased the content of sulforaphane in 7-d-old broccoli sprouts compared with the control. These results indicate that broccoli sprouts grown under a suitable concentration of naCl could be desirable for human nutrition.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Aires, A., Rosa, E., Carvalho, R., 2006. Effect of nitrogen and sulfur fertilization on glucosinolates in the leaves and roots of broccoli sprouts (Brassica oleracea var. italica). J. Sci. Food Agric., 86(10):1512-1516.

[2]Akhlaghi, M., Bandy, B., 2010. Dietary broccoli sprouts protect against myocardial oxidative damage and cell death during ischemia-reperfusion. Plant Foods Hum. Nutr., 65(3):193-199.

[3]Chen, S.X., Andreasson, E., 2001. Update on glucosinolate metabolism and transport. Plant Physiol. Biochem., 39(9):743-758.

[4]Fahey, J.W., Zhang, Y., Talalay, P., 1997. Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. PNAS, 94(19):10367-10372.

[5]Fahey, J.W., Zalcmann, A.T., Talalay, P., 2001. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry, 56(1):5-51.

[6]Francisco, M., Velasco, P., Moreno, D.A., García-Viguera, C., Cartea, M.E., 2010. Cooking methods of Brassica rapa affect the preservation of glucosinolates, phenolics and vitamin C. Food Res. Int., 43(5):1455-1463.

[7]Guo, R., Yuan, G., Wang, Q., 2011. Effect of sucrose and mannitol on the accumulation of health-promoting compounds and the activity of metabolic enzymes in broccoli sprouts. Sci. Hort., 128(3):159-165.

[8]Jones, R.B., Frisina, C.L., Winkler, S., Imsic, M., Tomkins, R.B., 2010. Cooking method significantly effects glucosinolate content and sulforaphane production in broccoli florets. Food Chem., 123(2):237-242.

[9]Khan, N.A., Nazar, R., Anjum, N.A., 2009. Growth, photosynthesis and antioxidant metabolism in mustard (Brassica juncea L.) cultivars differing in ATP-sulfurylase activity under salinity stress. Sci. Hort., 122(3):455-460.

[10]Kim, H.J., Chen, F., Wang, X., Choi, J.H., 2006. Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.). J. Agric. Food Chem., 54(19):7263-7269.

[11]Kissen, R., Rossiter, J.T., Bones, A.M., 2009. The ‘mustard oil bomb’: not so easy to assemble?! Localization, expression and distribution of the components of the myrosinase enzyme system. Phytochem. Rev., 8(1):69-86.

[12]López-Berenguer, C., Martinez-Ballesta, M.C., Garcia-Viguera, C., Carvajal, M., 2008. Leaf water balance mediated by aquaporins under salt stress and associated glucosinolate synthesis in broccoli. Plant Sci., 174(3):321-328.

[13]Nakagawa, K., Umeda, T., Higuchi, O., Tsuzuki, T., Suzuki, T., Miyazawa, T., 2006. Evaporative light-scattering analysis of sulforaphane in broccoli samples: quality of broccoli products regarding sulforaphane contents. J. Agric. Food Chem., 54(7):2479-2483.

[14]Nyberg, S., 1991. Multiple use of plants: studies on selenium incorporation in some agricultural species for the production of organic selenium compounds. Plant Foods Hum. Nutr., 41(1):69-88.

[15]Okulicz, M., 2010. Multidirectional time-dependent effect of sinigrin and allyl isothiocyanate on metabolic parameters in rats. Plant Foods Hum. Nutr., 65(3):217-224.

[16]Pereira, F.M., Rosa, E., Fahey, J.W., Stephenson, K.K., Carvalho, R., Aires, A., 2002. Influence of temperature and ontogeny on the levels of glucosinolates in broccoli (Brassica oleracea var. italica) sprouts and their effect on the induction of mammalian phase 2 enzymes. J. Agric. Food Chem., 50(21):6239-6244.

[17]Pérez-Balibrea, S., Moreno, D.A., García-Viguera, C., 2010. Glucosinolates in broccoli sprouts (Brassica oleracea var. italica) as conditioned by sulphate supply during germination. J. Food Sci., 75(8):673-677.

[18]Qasim, M., Ashraf, M., Ashraf, M.Y., Rehman, S.U., Rha, E.S., 2003. Salt-induced changes in two canola cultivars differing in salt tolerance. Biol. Plant., 46(4):629-632.

[19]Schreiner, M., Beyene, B., Krumbein, A., Stutzel, H., 2009. Ontogenetic changes of 2-propenyl and 3-indolylmethyl glucosinolates in Brassica carinata leaves as affected by water supply. J. Agric. Food Chem., 57(16):7259-7263.

[20]Scialabba, A., Melati, M.R., 1990. The effect of NaCl on growth and xylem differentiation of radish seedlings. Bot. Gaz., 151(4):516-521.

[21]Sharma, R., Sharma, A., Chaudhary, P., Pearce, V., Vatsyayan, R., Singh, S.V., Awasthi, S., Awasthi, Y.C., 2010. Role of lipid peroxidation in cellular responses to d,l-sulforaphane, a promising cancer chemopreventive agent. Biochemistry, 49(14):3191-3202.

[22]Singh, S.V., Warin, R., Xiao, D., Powolny, A.A., Stan, S.D., Arlotti, J.A., Zeng, Y., Hahm, E.R., Marynowski, S.W., Bommareddy, A., et al., 2009. Sulforaphane inhibits prostate carcinogenesis and pulmonary metastasis in tramp mice in association with increased cytotoxicity of natural killer cells. Cancer Res., 69(5):2117-2125.

[23]Textor, S., Gershenzon, J., 2009. Herbivore induction of the glucosinolate-myrosinase defense system: major trends, biochemical bases and ecological significance. Phytochem. Rev., 8(1):149-170.

[24]van Eylen, D., Hendrickx, I.M., van Loey, A., 2006. Temperature and pressure stability of mustard seed (Sinapis alba L.) myrosinase. Food Chem., 97(2):263-271.

[25]van Eylen, D., Oey, I., Hendrickx, M., van Loey, A., 2008. Effects of pressure/temperature treatments on stability and activity of endogenous broccoli (Brassica oleracea L. cv. italica) myrosinase and on cell permeability. J. Food Eng., 89(2):178-186.

[26]Velasco, P., Cartea, M.E., González, C., Vilar, M., Ordás, A., 2007. Factors affecting the glucosinolate content of kale (Brassica oleracea acephala group). J. Agric. Food Chem., 55(3):955-962.

[27]Yamada, K., Hasegawa, T., Minami, E., Shibuya, N., Kosemura, S., Yamamura, S., Hasegawa, K., 2003. Induction of myrosinase gene expression and myrosinase activity in radish hypocotyls by phototropic stimulation. J. Plant Physiol., 160(3):255-259.

[28]Yuan, G.F., Sun, B., Yuan, J., Wang, Q.M., 2009. Effect of different cooking methods on health-promoting compounds of broccoli. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 10(8):580-588.

[29]Yuan, G.F., Wang, X.P., Guo, R.F., Wang, Q.M., 2010. Effect of salt stress on phenolic compounds, glucosinolates, myrosinase and antioxidant activity in radish sprouts. Food Chem., 121(4):1014-1019.

[30]Zapata, P.J., Serrano, M., Pretel, A.T., Amoros, A., Botella, M.A., 2004. Polyamines and ethylene changes during germination of different plant species under salinity. Plant Sci., 167(4):781-788.

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