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On-line Access: 2013-01-08

Received: 2012-04-07

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.1 P.68-75


Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum

Author(s):  Xia-zhen Yu, Tao Ji, Xue-li Bai, Liang Liang, Lin-yan Wang, Wei Chen, Ting-bo Liang

Affiliation(s):  Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   liangtingbo@zju.edu.cn

Key Words:  Hepatic sinusoidal obstruction syndrome, Gynura segetum (Compositae), Sinusoidal endothelial cells, Matrix metalloproteinase-9

Xia-zhen Yu, Tao Ji, Xue-li Bai, Liang Liang, Lin-yan Wang, Wei Chen, Ting-bo Liang. Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum[J]. Journal of Zhejiang University Science B, 2013, 14(1): 68-75.

@article{title="Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum",
author="Xia-zhen Yu, Tao Ji, Xue-li Bai, Liang Liang, Lin-yan Wang, Wei Chen, Ting-bo Liang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum
%A Xia-zhen Yu
%A Tao Ji
%A Xue-li Bai
%A Liang Liang
%A Lin-yan Wang
%A Wei Chen
%A Ting-bo Liang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 1
%P 68-75
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200112

T1 - Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum
A1 - Xia-zhen Yu
A1 - Tao Ji
A1 - Xue-li Bai
A1 - Liang Liang
A1 - Lin-yan Wang
A1 - Wei Chen
A1 - Ting-bo Liang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 1
SP - 68
EP - 75
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200112

Background and objective: hepatic sinusoidal obstruction syndrome (HSOS) is characterized by painful hepatomegaly, ascites, increased body weight, and jaundice. Gynura segetum (Compositae), a plant widely used in Chinese traditional medicine, often leads to the development of HSOS. However, the mechanism is unclear. The aim was to study the role of matrix metalloproteinase-9 (MMP-9) in the onset of HSOS induced by Gynura segetum. Methods: Twenty-five male Sprague-Dawley rats were randomly divided into two groups. Twenty were exposed to 600 mg/kg daily Gynura segetum extract solution for three weeks; five control rats were exposed to tap water alone. Liver sections were evaluated by light microscopy with a modified scoring system. Routine transmission electron microscopy (TEM) methods were used to evaluate the ultrastructual features of fixed liver tissue, and blood samples were collected to determine liver enzyme concentrations. MMP-9 expression was assessed by both immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA) methods. Results: A stable and reproducible rat model of HSOS was achieved by long-term exposure to Gynura segetum extract. The treated rats presented clinical symptoms and the histopathological manifestation of HSOS, including abnormal liver enzyme concentrations (alanine aminotransferase (ALT): (84.8±13.62) vs. (167.0±72.63) U/L, P<0.05; aspartate aminotransferase (AST): (27.6±6.31) vs. (232.8±108.58) U/L, P<0.05). Hematoxylin and eosin (H&E) staining and TEM together revealed deposition of red blood cells, the damage and destruction of hepatic sinusoidal endothelial cells, collapse of hepatic sinusoids, hemorrhage of subendothelial cells, atrophy and destruction of hepatocytes, etc. Compared with controls, the expression of MMP-9 in the blood sample, the lung and liver tissues of HSOS rats was increased. Conclusions: MMP-9 may have an important role in early pathological changes of HSOS, and thus the onset of the disease.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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