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CLC number: R917

On-line Access: 2013-07-04

Received: 2012-12-27

Revision Accepted: 2013-01-22

Crosschecked: 2013-06-17

Cited: 3

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.7 P.615-620


Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase

Author(s):  Jie Zhou, Qian Liu, Guang-jun Fu, Zhen-zhong Zhang

Affiliation(s):  Key Laboratory of State Ministry of Education for Pharmaceutical Technology, School of Pharmacy, Zhengzhou University, Zhengzhou 450001, China

Corresponding email(s):   zhangzz08@126.com

Key Words:  CHIRALPAK®, IC, Enantiomeric separation, High-performance liquid chromatography (HPLC), Mandelic acid, Derivatives

Jie Zhou, Qian Liu, Guang-jun Fu, Zhen-zhong Zhang. Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase[J]. Journal of Zhejiang University Science B, 2013, 14(7): 615-620.

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author="Jie Zhou, Qian Liu, Guang-jun Fu, Zhen-zhong Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase
%A Jie Zhou
%A Qian Liu
%A Guang-jun Fu
%A Zhen-zhong Zhang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 7
%P 615-620
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200361

T1 - Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase
A1 - Jie Zhou
A1 - Qian Liu
A1 - Guang-jun Fu
A1 - Zhen-zhong Zhang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 7
SP - 615
EP - 620
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200361

A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chloromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAK®; IC (250 mm×4.6 mm, 5 μm). mandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid were baseline resolved (resolution factor (RS)=2.21, RS=2.14, and RS=3.70, respectively). In contrast, the enantioselectivities between CHIRALPAK®; IC and 2-chloromandelic acid and 4-hydroxymandelic acid investigated were low. By comparing the chromatographs of mandelic acid enantiomers and mandelic acid spiked with (R)-mandelic acid, it was determined that the first effluent was (R)-mandelic acid.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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