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Received: 2008-03-20

Revision Accepted: 2008-04-24

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.9 P.728-733


Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

Author(s):  Jin-zhong XU, Jie SHEN, Yi-yu CHENG, Hai-bin QU

Affiliation(s):  Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   quhb@zju.edu.cn

Key Words:  High-performance liquid chromatography (HPLC), Danshen injection (DSI), Phenolic acids, Multi-wavelengths, Quantification

Jin-zhong XU, Jie SHEN, Yi-yu CHENG, Hai-bin QU. Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector[J]. Journal of Zhejiang University Science B, 2008, 9(9): 728-733.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector
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%A Yi-yu CHENG
%A Hai-bin QU
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820095

T1 - Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector
A1 - Jin-zhong XU
A1 - Jie SHEN
A1 - Yi-yu CHENG
A1 - Hai-bin QU
J0 - Journal of Zhejiang University Science B
VL - 9
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0820095

A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008~0.160 μg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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