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CLC number: Q946.829

On-line Access: 2013-10-08

Received: 2012-12-29

Revision Accepted: 2013-06-03

Crosschecked: 2013-09-06

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.10 P.903-915

http://doi.org/10.1631/jzus.B1200365


Optimization of extraction of phenolics from leaves of Ficus virens


Author(s):  Xiao-xin Chen, Xiao-bing Wu, Wei-ming Chai, Hui-ling Feng, Yan Shi, Han-tao Zhou, Qing-xi Chen

Affiliation(s):  Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China

Corresponding email(s):   htzhou@xmu.edu.cn, chenqx@xmu.edu.cn

Key Words:  Ficus virens, Phenolics, Flavonoids, Antioxidants, Response surface methodology (RSM)


Xiao-xin Chen, Xiao-bing Wu, Wei-ming Chai, Hui-ling Feng, Yan Shi, Han-tao Zhou, Qing-xi Chen. Optimization of extraction of phenolics from leaves of Ficus virens[J]. Journal of Zhejiang University Science B, 2013, 14(10): 903-915.

@article{title="Optimization of extraction of phenolics from leaves of Ficus virens",
author="Xiao-xin Chen, Xiao-bing Wu, Wei-ming Chai, Hui-ling Feng, Yan Shi, Han-tao Zhou, Qing-xi Chen",
journal="Journal of Zhejiang University Science B",
volume="14",
number="10",
pages="903-915",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200365"
}

%0 Journal Article
%T Optimization of extraction of phenolics from leaves of Ficus virens
%A Xiao-xin Chen
%A Xiao-bing Wu
%A Wei-ming Chai
%A Hui-ling Feng
%A Yan Shi
%A Han-tao Zhou
%A Qing-xi Chen
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 10
%P 903-915
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200365

TY - JOUR
T1 - Optimization of extraction of phenolics from leaves of Ficus virens
A1 - Xiao-xin Chen
A1 - Xiao-bing Wu
A1 - Wei-ming Chai
A1 - Hui-ling Feng
A1 - Yan Shi
A1 - Han-tao Zhou
A1 - Qing-xi Chen
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 10
SP - 903
EP - 915
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200365


Abstract: 
In this research, the conditions for extraction of phenolics from leaves of Ficus virens were optimized using response surface methodology (RSM). The extraction abilities of phenolics (EAP) and flavonoids (EAF), the 2,2-diphenyl-1-pierylhydrazyl (DPPH) free-radical scavenging potential, and the ferric reducing/antioxidant power (FRAP) were used as quality indicators. The results of single-factor experiments showed that temperature, ethanol concentration, extraction time, and the number of extraction cycles were the main influencing variables, and these provided key information for the central composite design. The results of RSM fitted well to a second degree polynomial model and more than 98% of the variability was explained. The ideal extraction conditions for EAP, EAF, DPPH free-radical scavenging potential, and FRAP were obtained. Considering the four quality indicators overall, the ideal extraction conditions were 58% ethanol at 57 °C for 37 min with three extraction cycles. At the ideal extraction conditions, the values of EAP, EAF, DPPH free-radical scavenging potential, and FRAP were 5.72%, 3.09%, 58.88 mg ascorbic acid equivalent (AAE)/g dry weight (DW), and 15.86 mg AAE/g DW, respectively. In addition, linear correlations were observed between EAP, EAF, and antioxidant potential.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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