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CLC number: R780.1

On-line Access: 2014-03-04

Received: 2013-03-22

Revision Accepted: 2013-05-07

Crosschecked: 2014-02-11

Cited: 3

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.3 P.289-294


Effect of vitamin B12 on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice*

Author(s):  Shu-fan Zhao1,2, Mao-zhou Chai1,2, Min Wu3, Yong-hong He1, Tian Meng1,2, Bing Shi1,2

Affiliation(s):  1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; more

Corresponding email(s):   menglj123@126.com

Key Words:  Cleft palate, Transforming growth factor-&beta, 3 (TGF-&beta, 3), Activin receptor-like kinase 5 (ALK5), Vitamin B12 , 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin, Dexamethasone

Shu-fan Zhao, Mao-zhou Chai, Min Wu, Yong-hong He, Tian Meng, Bing Shi. Effect of vitamin B12 on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice[J]. Journal of Zhejiang University Science B, 2014, 15(3): 289-294.

@article{title="Effect of vitamin B12 on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice",
author="Shu-fan Zhao, Mao-zhou Chai, Min Wu, Yong-hong He, Tian Meng, Bing Shi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of vitamin B12 on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice
%A Shu-fan Zhao
%A Mao-zhou Chai
%A Min Wu
%A Yong-hong He
%A Tian Meng
%A Bing Shi
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 3
%P 289-294
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300083

T1 - Effect of vitamin B12 on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice
A1 - Shu-fan Zhao
A1 - Mao-zhou Chai
A1 - Min Wu
A1 - Yong-hong He
A1 - Tian Meng
A1 - Bing Shi
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 3
SP - 289
EP - 294
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300083

The purpose of this study was to investigate the effect of 28vitamin B12%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>vitamin B12 on palatal development by co-administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and dexamethasone (DEX). We examined the morphological and histological features of the palatal shelf and expression levels of key signaling molecules (28transforming growth factor-&beta%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>transforming growth factor-&beta;283 (TGF-&beta%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>3 (TGF-&beta;283)%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>3) and TGF-β type I receptor (activin receptor-like kinase 5, ALK5)) during palatogenesis among a control group (Group A), TCDD+DEX exposed group (Group B), and TCDD+DEX+28vitamin B12%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>vitamin B12 exposed group (Group C). While we failed to find that 28vitamin B12%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>vitamin B12 decreased the incidence of 28cleft palate%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>cleft palate induced by TCDD+DEX treatment, the expression levels of key signaling molecules (TGF-β3 and ALK5) during palatogenesis were significantly modulated. In TCDD+DEX exposed and TCDD+DEX+28vitamin B12%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>vitamin B12 exposed groups, palatal shelves could not contact in the midline due to their small sizes. Our results suggest that 28vitamin B12%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>vitamin B12 may inhibit the expression of some 28cleft palate%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>cleft palate inducers such as TGF-β3 and ALK5 in DEX+TCDD exposed mice, which may be beneficial against palatogenesis to some degree, even though we were unable to observe a protective role of 28vitamin B12%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>2f4a8b>vitamin B12 in morphological and histological alterations of palatal shelves induced by DEX and TCDD.


研究方法:在小鼠腭发育关键时期,采用高剂量TCDD和DEX一次给药建立模型,并观察维生素B12的拮抗作用(见图3)。于胚胎期17.5天(GD 17.5)体视显微镜下检测各组腭裂发生率(见表1和图2),并于GD 13.5、GD 14.5、GD 15.5 分别剪取胎鼠腭突提取RNA,采用实时荧光定量聚合酶链反应检测TGF-β3和ALK5基因表达(见图4、5)。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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