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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.11 P.813-819


Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Author(s):  Izatus Shima TAIB, Siti Balkis BUDIN, Seri Maseran SITI NOR AIN, Jamaludin MOHAMED, Santhana Raj LOUIS, Srijit DAS, Sulaiman SALLEHUDIN, Nor Fadilah RAJAB, Othman HIDAYATULFATHI

Affiliation(s):  Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia; more

Corresponding email(s):   balkis6466@yahoo.com.my

Key Words:  Litsea elliptica, Toxicity, Red blood cells, Morphology, Cell membrane, Echinocyte

Izatus Shima TAIB, Siti Balkis BUDIN, Seri Maseran SITI NOR AIN, Jamaludin MOHAMED, Santhana Raj LOUIS, Srijit DAS, Sulaiman SALLEHUDIN, Nor Fadilah RAJAB, Othman HIDAYATULFATHI. Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats[J]. Journal of Zhejiang University Science B, 2009, 10(11): 813-819.

@article{title="Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats",
author="Izatus Shima TAIB, Siti Balkis BUDIN, Seri Maseran SITI NOR AIN, Jamaludin MOHAMED, Santhana Raj LOUIS, Srijit DAS, Sulaiman SALLEHUDIN, Nor Fadilah RAJAB, Othman HIDAYATULFATHI",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats
%A Izatus Shima TAIB
%A Siti Balkis BUDIN
%A Seri Maseran SITI NOR AIN
%A Jamaludin MOHAMED
%A Santhana Raj LOUIS
%A Srijit DAS
%A Nor Fadilah RAJAB
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 11
%P 813-819
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920199

T1 - Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats
A1 - Izatus Shima TAIB
A1 - Siti Balkis BUDIN
A1 - Seri Maseran SITI NOR AIN
A1 - Jamaludin MOHAMED
A1 - Santhana Raj LOUIS
A1 - Srijit DAS
A1 - Sulaiman SALLEHUDIN
A1 - Nor Fadilah RAJAB
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 11
SP - 813
EP - 819
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920199

Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, chemopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat’s red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Abreu, P.R., Almeida, M.C., Bernardo, R.M., Bernardo, L.C., Brito, L.C., Garcia, E.A., Fonseca, A.S., Bernardo-Filho, M., 2006. Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m. J. Zhejiang Univ. Sci. B, 7(6):429-435.

[2] Álvarez, L., Gil, A.G., Ezpeleta, O., Jalon-Garcia, J.A., Cerain, L., 2004. Immunotoxic effects of Ochratoxin A in Wistar rats after oral administration. Food Chem. Toxicol., 42(5): 825-834.

[3] Bent, S., Ko, R., 2004. Commonly used herbal medicines in the United States: a review. Am. J. Med., 116(7):478-485.

[4] Bhamarapravati, S., Pendland, S.L., Mahady, G.B., 2003. Extracts of spice and food plants from Thai traditional medicine inhibit the growth of the human carcinogen Helicobacter pylori. In Vivo, 17(6):541-544.

[5] Celik, I., Suzek, H., 2008. The hematological effects of methyl parathion in rats. J. Hazard. Mater., 153(3):1117-1121.

[6] Cynamon, H.A., Isenbergs, J.N., Nguyen, C.H., 1984. A rapid method for erythrocytes membrane phospholipids determination. Clin. Chim. Acta, 144(1):65-70.

[7] Da Silva, J., Herrmann, S.M., Heuser, V., Peres, W., Marroni, N.P., Gonzalez-Gallego, J., Erdtmann, B., 2002. Evaluation of the genotoxic affect of rutin and quercetina by comet assay and micronucleus test. Food Chem. Toxicol., 40(7):941-947.

[8] Darcie, S.J.V., Lewis, S.M., 1994. Practical Haematology. ELBS, Churchill Livingstone, Leith Walk, Edinburgh, p.215-247.

[9] Eisenberg, D.M., Davis, R.B., Ettener, S.L., 1998. Trends in alternative medicine use in the United States, 1990–1997: results of a follow-up national survey. JAMA, 280(18): 1569-1575.

[10] Folch, J., Lees, M., Sloane, S.G.H., 1957. A simple method for isolation and purification of total lipids from animal tissue. J. Chem. Biol., 226(1):497-509.

[11] Grosvenor, P.W., Gothard, P.K., McWilliam, N.C., Supriono, A., Gray, D.O., 1995. Medicinal plants from Riau Province, Sumatra, Indonesia. Part 1: Uses. J. Ethnopharmacol., 45(2):75-95.

[12] Hasumura, M., Yasuhara, K., Tamura, T., Imai, T., Mitsumori, K., Hirose, M., 2004. Evaluation of the toxicity of enzymatically decomposed rutin with 13-weeks dietary administration to Wistar rats. Food Chem. Toxicol., 42(3): 439-444.

[13] Hidayatulfathi, O., Sallehudin, S., Ibrahim, J., Azizol, A.K., 2003. Evaluation of methanol extracts of some Malaysian plants for larvicidal activities. Trop. Biomed., 21(2): 153-157.

[14] Hidayatulfathi, O., Sallehudin, S., Ibrahim, J., 2004. Adulticidal activity of some Malaysian plant extracts against Aedes aegypti Linnaeus. Trop. Biomed., 21:61-67.

[15] Janbaz, K.H., Saeed, S.A., Gilani, A.H., 2002. Protective effect of rutin on paracetamol and CCl4-induced hepatotoxicity in rodents. Fitoterapia, 73(7-8):557-663.

[16] Jantan, I., Zaki, Z.M., 1998. Development of Environment-Friendly Insect Repellents from the Leaf Oils of Selected Malaysian Plants. ASEAN Review of Biodiversity and Environmental Conversation (ARBEC). May, p.1-7. Available from http://www.arbec.com.my/pdf/may (accessed on Oct. 13, 2009).

[17] Jiwajinda, S., Santisopasri, V., Murakami, A., Kawanaka, M., Kawanaka, H., Gasquet, M., Eilas, R., Balansard, G., Ohigashi, H., 2002. In vitro anti-tumor promoting and anti-parasitic activities of the quassinoids from Eurycoma longifolia, a medicinal plant in Southeast Asia. J. Ethnopharmacol., 82(1):55-58.

[18] Jokinen, C.H., Swaim, W.R., Nuttall, F.Q., 2004. A case of hereditary xerocytosis diagnosed as a result of suspected hypoglycemia and observed low glycohemoglobin. J. Lab. Clin. Med., 144(1):27-30.

[19] Kolanjiappan, K., Manoharan, S., Kayalvizhi, M., 2002. Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clin. Chim. Acta, 326(1-2):143-149.

[20] Mahieu, S., Contini, M.C., Gonzalez, M., Millen, N., Elias, M.M., 2000. Aluminium toxicity. Hematological effects. Toxicol. Lett., 111(3):235-242.

[21] Mittal, P.K., Subbarao, S.K., 2003. Prospects of using herbal products in the control ofmosquito vector. ICMR Bull, 33(1):1-10.

[22] Nakahara, K., Trakoontivakorn, G., Alzoreky, N.S., Ono, H., Onishi-Kameyama, M., Yoshida, M., 2002. Antimutagenecity of some edible Thai plants, and bioactive carbazole alkaloid, mahanine, isolated from Micromelum minutum. J. Agric. Food Chem., 50(17):4796-4802.

[23] Nehez, M., Toth, C.S., Desi, I., 1994. The effect of dimethoate, dichlorvos, and parathion-methyl on bone marrow cell chromosomes of rats in subchronic experiments in vivo. Ecotoxicol. Environ. Saf., 29(3):365-371.

[24] OECD, 2000. Guidance Notes for Analysis and Evaluation of Repeat-Dose Toxicity Studies. OECD Series on Testing and Assessment Number 23 and OECD Series on Pesticides Number 10. Health and Safety Publication. OECD Environment, Paris. Available from website http://www.oecd.org/dataoecd/47/29/2765785

[25] Oteiza, P.I., 1994. A mechanism for the stimulatory effect of aluminum on iron-induced lipid peroxidation. Arch. Biochem. Biophys., 308(2):374-379.

[26] Przybylska, M., Faber, M., Zaborowski, A., Swietoslawski, J., Bryszewska, M., 1998. Morphological changes of human RBC induced by cholesterol sulphate. Clin. Biochem., 31(2):73-79.

[27] Rohani, A., Nazni, W.A., Ngo, L.V., Ibrahim, J., Lee, H.L., 1997. Adulticidal properties of the essential extracts of some Malaysian plants on vector mosquitoes. Trop. Biomed., 14:5-9.

[28] Sanderson, J.H., Philips, C.E., 1981. An Atlas of Laboratory Animal Haematology. Oxford University Press, New York, p.471.

[29] Sheetz, M.P., Singer, S.J., 1974. Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interaction. Proc. Natl. Acad. Sci., 71(11): 4457-4461.

[30] Shi, Y.Z., Ruan, J.Y., Ma, L.F., Han, W.Y., Wang, F., 2008. Accumulation and distribution of arsenic and cadmium by tea plants. J. Zhejiang Univ. Sci. B, 9(3):265-270.

[31] Shiva Shankar Reddy, C.S., Subramanyam, M.V.V., Vani, R., Asha Devi, S., 2007. In vitro models of oxidative stress in rat erythrocytes: effect of antioxidant supplements. Toxicol. in Vitro, 21(8):1355-1364.

[32] Siti Nor Ain, S.M., 2008. Safety Evaluation of Litsea elliptica Blume (Lauraceae) Essential Oil Using Acute and Subacute Toxicity Study via Oral Route on Sprague-Dawley Rats. Available from http://www.fskb.ukm.my/SENARAI%20TESIS%20PELAJAR%20SARJANA%20FSKB.pdf (accessed on Oct. 13, 2009).

[33] Suwalsky, M., Orellana, P., Avello, M., Villena, F., Sotomayor, C.P., 2006. Human erythrocytes are affected in vitro by extracts of Ugni molinae leaves. Food. Chem. Toxicol., 44(8):1393-1398.

[34] Suwalsky, M., Vargas, P., Avello, M., Villena, F., Sotomayor, C.P., 2008. Human erythrocytes are affected in vitro by flavonoids of Aristotelia chilensis (Maqui) leaves. Int. J. Pharm., 363(1-2):85-90.

[35] Talalay, P., Talalay, P., 2001. The importance of using scientific principles in the development of medicinal agents from plants. Acad. Med., 76(3):238-247.

[36] Udden, M.M., 2005. Effects of diethylene glycol butyl ether and butoxyethoxyacetic acid on rat and human RBC. Toxicol. Lett., 156(1):95-101.

[37] WHO (World Health Organization), 1995. Pesticides Residues in Food—1995, Joint FAO/WHO. Meeting on Pesticides Residues in Food. Part II: Toxicological and Environmental. Available from http://www.who.int/ipcs/publications/jmpr/en (accessed on Oct. 13, 2009).

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