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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.11 P.813-819


Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Author(s):  Izatus Shima TAIB, Siti Balkis BUDIN, Seri Maseran SITI NOR AIN, Jamaludin MOHAMED, Santhana Raj LOUIS, Srijit DAS, Sulaiman SALLEHUDIN, Nor Fadilah RAJAB, Othman HIDAYATULFATHI

Affiliation(s):  Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia; more

Corresponding email(s):   balkis6466@yahoo.com.my

Key Words:  Litsea elliptica, Toxicity, Red blood cells, Morphology, Cell membrane, Echinocyte

Izatus Shima TAIB, Siti Balkis BUDIN, Seri Maseran SITI NOR AIN, Jamaludin MOHAMED, Santhana Raj LOUIS, Srijit DAS, Sulaiman SALLEHUDIN, Nor Fadilah RAJAB, Othman HIDAYATULFATHI. Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats[J]. Journal of Zhejiang University Science B, 2009, 10(11): 813-819.

@article{title="Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats",
author="Izatus Shima TAIB, Siti Balkis BUDIN, Seri Maseran SITI NOR AIN, Jamaludin MOHAMED, Santhana Raj LOUIS, Srijit DAS, Sulaiman SALLEHUDIN, Nor Fadilah RAJAB, Othman HIDAYATULFATHI",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats
%A Izatus Shima TAIB
%A Siti Balkis BUDIN
%A Seri Maseran SITI NOR AIN
%A Jamaludin MOHAMED
%A Santhana Raj LOUIS
%A Srijit DAS
%A Nor Fadilah RAJAB
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 11
%P 813-819
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920199

T1 - Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats
A1 - Izatus Shima TAIB
A1 - Siti Balkis BUDIN
A1 - Seri Maseran SITI NOR AIN
A1 - Jamaludin MOHAMED
A1 - Santhana Raj LOUIS
A1 - Srijit DAS
A1 - Sulaiman SALLEHUDIN
A1 - Nor Fadilah RAJAB
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 11
SP - 813
EP - 819
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920199

Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, chemopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat’s red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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