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On-line Access: 2015-06-08

Received: 2014-11-30

Revision Accepted: 2015-04-08

Crosschecked: 2015-05-13

Cited: 8

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Citations:  Bibtex RefMan EndNote GB/T7714


Li Min


Nan Zheng


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.541-548


Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows

Author(s):  Li Min, Jian-bo Cheng, Bao-lu Shi, Hong-jian Yang, Nan Zheng, Jia-qi Wang

Affiliation(s):  Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; more

Corresponding email(s):   zhengnan_1980@126.com

Key Words:  Heat stress, Dairy cows, Serum, Temperature-humidity index (THI), Biomarkers

Li Min, Jian-bo Cheng, Bao-lu Shi, Hong-jian Yang, Nan Zheng, Jia-qi Wang. Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows[J]. Journal of Zhejiang University Science B, 2015, 16(6): 541-548.

@article{title="Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows",
author="Li Min, Jian-bo Cheng, Bao-lu Shi, Hong-jian Yang, Nan Zheng, Jia-qi Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows
%A Li Min
%A Jian-bo Cheng
%A Bao-lu Shi
%A Hong-jian Yang
%A Nan Zheng
%A Jia-qi Wang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 541-548
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400341

T1 - Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows
A1 - Li Min
A1 - Jian-bo Cheng
A1 - Bao-lu Shi
A1 - Hong-jian Yang
A1 - Nan Zheng
A1 - Jia-qi Wang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 541
EP - 548
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400341

heat stress affects feed intake, milk production, and endocrine status in dairy cows. The temperature-humidity index (THI) is employed as an index to evaluate the degree of heat stress in dairy cows. However, it is difficult to ascertain whether THI is the most appropriate measurement of heat stress in dairy cows. This experiment was conducted to investigate the effects of heat stress on serum insulin, adipokines (leptin and adiponectin), AMP-activated protein kinase (AMPK), and heat shock signal molecules (heat shock transcription factor (HSF) and heat shock proteins (HSP)) in dairy cows and to research biomarkers to be used for better understanding the meaning of THI as a bioclimatic index. To achieve these objectives, two experiments were performed. The first experiment: eighteen lactating Holstein dairy cows were used. The treatments were: heat stress (HS, THI average=81.7, n=9) and cooling (CL, THI average=53.4, n=9). Samples of HS were obtained on August 16, 2013, and samples of CL were collected on April 7, 2014 in natural conditions. The second experiment: HS treatment cows (n=9) from the first experiment were fed for 8 weeks from August 16, 2013 to October 12, 2013. Samples for moderate heat stress, mild heat stress, and no heat stress were obtained, respectively, according to the physical alterations of the THI. Results showed that heat stress significantly increased the serum adiponectin, AMPK, HSF, HSP27, HSP70, and HSP90 (P<0.05). Adiponectin is strongly associated with AMPK. The increases of adiponectin and AMPK may be one of the mechanisms to maintain homeostasis in heat-stressed dairy cows. When heat stress treatment lasted 8 weeks, a higher expression of HSF and HSP70 was observed under moderate heat stress. serum HSF and HSP70 are sensitive and accurate in heat stress and they could be potential indicators of animal response to heat stress. We recommend serum HSF and HSP70 as meaningful biomarkers to supplement the THI and evaluate moderate heat stress in dairy cows in the future.




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