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CLC number: R374.2

On-line Access: 2016-04-05

Received: 2015-04-16

Revision Accepted: 2015-11-07

Crosschecked: 2016-03-15

Cited: 0

Clicked: 2650

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yue Feng

http://orcid.org/0000-0002-2718-0836

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.4 P.311-316

http://doi.org/10.1631/jzus.B1500091


Prevalence and genotype of Chlamydia psittaci in faecal samples of birds from zoos and pet markets in Kunming, Yunnan, China


Author(s):  Yue Feng, Yue-mei Feng, Zhong-hua Zhang, Shao-xiong Wu, Du-bo Zhong, Chen-jian Liu

Affiliation(s):  Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; more

Corresponding email(s):   newstaar8@hotmail.com

Key Words:  Chlamydia psittaci, Genotype, OmpA, Prevalence, Yunnan


Yue Feng, Yue-mei Feng, Zhong-hua Zhang, Shao-xiong Wu, Du-bo Zhong, Chen-jian Liu. Prevalence and genotype of Chlamydia psittaci in faecal samples of birds from zoos and pet markets in Kunming, Yunnan, China[J]. Journal of Zhejiang University Science B, 2016, 17(4): 311-316.

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author="Yue Feng, Yue-mei Feng, Zhong-hua Zhang, Shao-xiong Wu, Du-bo Zhong, Chen-jian Liu",
journal="Journal of Zhejiang University Science B",
volume="17",
number="4",
pages="311-316",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500091"
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%0 Journal Article
%T Prevalence and genotype of Chlamydia psittaci in faecal samples of birds from zoos and pet markets in Kunming, Yunnan, China
%A Yue Feng
%A Yue-mei Feng
%A Zhong-hua Zhang
%A Shao-xiong Wu
%A Du-bo Zhong
%A Chen-jian Liu
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A1 - Shao-xiong Wu
A1 - Du-bo Zhong
A1 - Chen-jian Liu
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DOI - 10.1631/jzus.B1500091


Abstract: 
Chlamydia psittaci is an important zoonotic pathogen in birds and may be transmitted to humans and result in severe respiratory disease. To assess the prevalence and genotype of C. psittaci in birds in Kunming, yunnan, China, a total of 136 specimens of psittacine birds involving 8 species were collected from the city’s zoos (n=60) and pet markets (n=76). The frequency of C. psittaci infection was 19.9% (27/136) in the psittacine birds. The prevalence of C. psittaci was higher in pet birds (26.3%; 20/76) than in zoo birds (11.7%; 7/60) (P=0.034). In particular, among Agapornis fischeri, the C. psittaci infection (50%; 10/20) was significantly more frequent in the pet markets than in the zoos (P=0.006). In addition, the highest prevalence of 41.2% (7/17) was found in Ara ararauna. To determine the genotype of C. psittaci, 23 OmpA gene fragments (about 1.4 kb) in 27 positive samples were successfully amplified and sequenced. Phylogenetic analysis showed that all the 23 strains belonged to genotype A. Our results demonstrate the high prevalence of C. psittaci genotype A infection in psittacine birds in yunnan Province, suggesting a potential threat to human health in this area. Therefore, it is necessary to take effective measures to prevent the spread of C. psittaci among psittacine birds, as well as among employees and customers.

云南省昆明市动物园和宠物市场中鸟类鹦鹉热衣原体的流行病学调查及其基因型分布研究

目的:调查云南省昆明市动物园和宠物市场的鹦形目鸟类中鹦鹉热衣原体的流行情况。
创新点:首次在云南地区开展鹦形目鸟类中鹦鹉热衣原体的流行病学调查,发现该地区鹦形目鸟类中具有较高的鹦鹉热衣原体感染率。
方法:本研究共采集8种鹦形目鸟类的新鲜粪便样本136份,其中动物园样本60份,宠物市场样本76份。首先,利用高灵敏和高特异性的TaqMan MGB探针荧光定量聚合酶链反应(qPCR)方法检测粪便样品中鹦鹉热衣原体的感染率,然后针对阳性样品,利用PCR技术进行鹦鹉热衣原体OmpA基因扩增、纯化、测序以及基因型分析。
结论:本实验结果显示:鹦鹉热衣原体的感染率为19.9% (27/136),其中宠物市场鸟类中鹦鹉热衣原体的感染率(26.3%;20/76)明显高于动物园(11.7%;7/60)(P=0.034);金刚鹦鹉中感染率最高,达41.2%(7/17)。同缘关系进化树分析表明,这些鹦鹉热衣原体都属于易于由鸟向人跨物种传播的A型。综上所述,昆明地区鹦形目鸟类中具有较高的鹦鹉热衣原体流行,给饲养人员和观鸟者的健康带来了潜在的威胁。

关键词:鹦鹉热衣原体;基因型;OmpA基因;流行病调查;云南

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