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Suppl. Mater.: 

CLC number: Q291

On-line Access: 2015-12-04

Received: 2015-06-21

Revision Accepted: 2015-11-07

Crosschecked: 2015-11-16

Cited: 1

Clicked: 4085

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hong-chang Liu

http://orcid.org/0000-0002-0797-6095

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.12 P.1027-1041

http://doi.org/10.1631/jzus.B1500150


Transcriptome changes in Polygonum multiflorum Thunb. roots induced by methyl jasmonate


Author(s):  Hong-chang Liu, Wei Wu, Kai Hou, Jun-wen Chen, Zhi Zhao

Affiliation(s):  College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China; more

Corresponding email(s):   ewuwei@sicau.edu.cn

Key Words:  Polygonum multiflorum Thunb., Methyl jasmonate, Transcriptome change, Differentially expressed genes


Hong-chang Liu, Wei Wu, Kai Hou, Jun-wen Chen, Zhi Zhao. Transcriptome changes in Polygonum multiflorum Thunb. roots induced by methyl jasmonate[J]. Journal of Zhejiang University Science B, 2015, 16(12): 1027-1041.

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author="Hong-chang Liu, Wei Wu, Kai Hou, Jun-wen Chen, Zhi Zhao",
journal="Journal of Zhejiang University Science B",
volume="16",
number="12",
pages="1027-1041",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500150"
}

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%T Transcriptome changes in Polygonum multiflorum Thunb. roots induced by methyl jasmonate
%A Hong-chang Liu
%A Wei Wu
%A Kai Hou
%A Jun-wen Chen
%A Zhi Zhao
%J Journal of Zhejiang University SCIENCE B
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%DOI 10.1631/jzus.B1500150

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T1 - Transcriptome changes in Polygonum multiflorum Thunb. roots induced by methyl jasmonate
A1 - Hong-chang Liu
A1 - Wei Wu
A1 - Kai Hou
A1 - Jun-wen Chen
A1 - Zhi Zhao
J0 - Journal of Zhejiang University Science B
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EP - 1041
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1500150


Abstract: 
Transcriptome profiling has been widely used to analyze transcriptomic variation in plants subjected to abiotic or biotic stresses. Although gene expression changes induced by methyl jasmonate (MeJA) have been profiled in several plant species, no information is available on the MeJA-triggered transcriptome response of Polygonum multiflorum Thunb., a species with highly valuable medicinal properties. In this study, we used transcriptome profiling to investigate transcriptome changes in roots of P. multiflorum seedlings subjected to a 0.25 mmol/L-MeJA root-irrigation treatment. A total of 18 677 differentially expressed genes (DEGs) were induced by MeJA treatment, of which 4535 were up-regulated and 14 142 were down-regulated compared with controls. These DEGs were associated with 125 metabolic pathways. In addition to various common primary and secondary metabolic pathways, several secondary metabolic pathways related to components with significant pharmacological effects were enriched by MeJA, including arachidonic acid metabolism, linoleic acid metabolism, and stilbenoid biosynthesis. The MeJA-induced transcriptome changes uncovered in this study provide a solid foundation for future study of functional genes controlling effective components in secondary metabolic pathways of P. multiflorum.

茉莉酸甲酯处理对何首乌根转录组的影响

目的:研究外源茉莉酸甲酯(MeJA)处理对中药何首乌(Polygonum multiflorum)根转录组的影响,分析在外源MeJA刺激下何首乌根各代谢途径中差异表达基因的变化,为寻找影响何首乌根主要药效成分合成的关键酶基因奠定基础。
创新点:首次在中药何首乌上利用外源MeJA处理研究何首乌根转录组的变化,且获得了根中各代谢途径的大量差异表达基因。
方法:将何首乌植株分为对照组(Hoagland营养液灌根)和实验组(含0.25 mmol/L MeJA的Hoagland营养液灌根)。灌根处理26 h后,分别提取对照组和实验组根中的总RNA,利用新一代测序技术分析对照组和实验组根的转录组变化并进行测序。同时,利用美国国家生物技术信息中心(NCBI)的蛋白质数据库和基因数据库信息,对所测cDNA序列进行基因功能注释和代谢途径归类,寻找MeJA刺激下何首乌根各条代谢途径中的差异表达基因。
结论:外源MeJA刺激处理可影响中药何首乌根转录组的表达变化(表S1)。与对照相比,外源MeJA处理可导致何首乌根中4535个基因上调,14 142个基因下调变化,并分别在125个代谢途径中富集(表1)。代谢途径差异表达基因分析的结果表明,可在相应代谢途径中找到影响何首乌某些主要药效成分合成的关键酶基因(图2)。差异表达基因的代谢途径归类分析结果提示今后可对何首乌根各代谢途径中的关键酶基因分别进行研究(表1)。综上所述,利用外源MeJA处理后进行转录组测序对寻找何首乌中主要有效成份合成的关键酶基因有重要价值。

关键词:何首乌;茉莉酸甲酯;转录组变化;差异表达基因

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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