CLC number: S476
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2016-07-13
Cited: 0
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Ya-qian Li, Kai Song, Ya-chai Li, Jie Chen. Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation[J]. Journal of Zhejiang University Science B, 2016, 17(8): 619-627.
@article{title="Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation",
author="Ya-qian Li, Kai Song, Ya-chai Li, Jie Chen",
journal="Journal of Zhejiang University Science B",
volume="17",
number="8",
pages="619-627",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500226"
}
%0 Journal Article
%T Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation
%A Ya-qian Li
%A Kai Song
%A Ya-chai Li
%A Jie Chen
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 8
%P 619-627
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500226
TY - JOUR
T1 - Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation
A1 - Ya-qian Li
A1 - Kai Song
A1 - Ya-chai Li
A1 - Jie Chen
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 8
SP - 619
EP - 627
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500226
Abstract: Trichoderma-based formulations are applied as commercial biocontrol agents for soil-borne plant pathogens. chlamydospores are active propagules in Trichoderma spp., but their production is currently limited due to a lack of optimal liquid fermentation technology. In this study, we explored response surface methodologies for optimizing fermentation technology in Trichoderma SH2303. Our initial studies, using the Plackett-Burman design, identified cornmeal, glycerol, and initial pH levels as the most significant factors (P<0.05) for enhancing the production of chlamydospores. Subsequently, we applied the box-Behnken design to study the interactions between, and optimal levels of, a number of factors in chlamydospore production. These statistically predicted results indicated that the highest number of chlamydospores (3.6×108 spores/ml) would be obtained under the following condition: corn flour 62.86 g/L, glycerol 7.54 ml/L, pH 4.17, and 6-d incubation in liquid fermentation. We validated these predicted values via three repeated experiments using the optimal culture and achieved maximum chlamydospores of 4.5×108 spores/ml, which approximately a 8-fold increase in the number of chlamydospores produced by T. harzianum SH2303 compared with that before optimization. These optimized values could help make chlamydospore production cost-efficient in the future development of novel biocontrol agents.
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