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CLC number: Q2-33

On-line Access: 2016-10-02

Received: 2015-09-25

Revision Accepted: 2016-02-16

Crosschecked: 2016-09-10

Cited: 1

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Citations:  Bibtex RefMan EndNote GB/T7714


Yuan-yuan Xu


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.10 P.807-812


Detection of biological thiols based on a colorimetric method

Author(s):  Yuan-yuan Xu, Yang-yang Sun, Yu-juan Zhang, Chen-he Lu, Jin-feng Miao

Affiliation(s):  Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Corresponding email(s):   mjf171647@126.com

Key Words:  Biothiols, Colorimetric detection, Bovine plasma, Cell lines

Yuan-yuan Xu, Yang-yang Sun, Yu-juan Zhang, Chen-he Lu, Jin-feng Miao. Detection of biological thiols based on a colorimetric method[J]. Journal of Zhejiang University Science B, 2016, 17(10): 807-812.

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publisher="Zhejiang University Press & Springer",

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%T Detection of biological thiols based on a colorimetric method
%A Yuan-yuan Xu
%A Yang-yang Sun
%A Yu-juan Zhang
%A Chen-he Lu
%A Jin-feng Miao
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%DOI 10.1631/jzus.B1500232

T1 - Detection of biological thiols based on a colorimetric method
A1 - Yuan-yuan Xu
A1 - Yang-yang Sun
A1 - Yu-juan Zhang
A1 - Chen-he Lu
A1 - Jin-feng Miao
J0 - Journal of Zhejiang University Science B
VL - 17
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1500232

Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.




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