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On-line Access: 2016-11-03

Received: 2016-03-27

Revision Accepted: 2016-07-13

Crosschecked: 2016-10-18

Cited: 3

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Fang-xiong Shi


Nazar Ali Korejo


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.11 P.850-863


Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals

Author(s):  Nazar Ali Korejo, Quan-wei Wei, Atta Hussain Shah, Fang-xiong Shi

Affiliation(s):  Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; more

Corresponding email(s):   fxshi@njau.edu.cn

Key Words:  Diabetes, Hyperthyroidism, Testicular and epididymal morphology

Nazar Ali Korejo, Quan-wei Wei, Atta Hussain Shah, Fang-xiong Shi. Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals[J]. Journal of Zhejiang University Science B, 2016, 17(11): 850-863.

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author="Nazar Ali Korejo, Quan-wei Wei, Atta Hussain Shah, Fang-xiong Shi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals
%A Nazar Ali Korejo
%A Quan-wei Wei
%A Atta Hussain Shah
%A Fang-xiong Shi
%J Journal of Zhejiang University SCIENCE B
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%P 850-863
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600136

T1 - Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals
A1 - Nazar Ali Korejo
A1 - Quan-wei Wei
A1 - Atta Hussain Shah
A1 - Fang-xiong Shi
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 11
SP - 850
EP - 863
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600136

This study evaluated the effects of comorbid disorders of diabetes and hyperthyroidism in the adult male mice. In total, 32 ICR strain mice were equally distributed into four groups: control (C), diabetic (D), diabetic-plus-hyperthyroid (DH), and hyperthyroid (H). Mice allocated for diabetes received a single intraperitoneal injection of streptozotocin (STZ) at 200 mg/kg body weight. At the onset of diabetes, one group of mice was concomitantly injected levothyroxine (LT4; 0.3 mg/kg body weight) and the other set of animals received the same treatment independently on a daily basis. The body weight, as well as the testicular and epididymal weights, was reduced markedly in D and DH mice. Higher trends of blood glucose levels were seen in the DH group, in comparison to euthyroid diabetic mice. Thyroid hormones could exert a transient effect on blood glucose homeostasis by altering the serum blood glucose level in diabetic patients. Histomorphometric analysis showed increased luminal sizes of seminiferous tubules, along with decreased epithelial height and atrophic changes in germinal stem cells in the testis of DH and H mice. Caput epididymis of DH mice showed extensive compaction of principal cells, loss of stereocilia, lipid vacuolization, and inflammatory infiltrations; however, damaged tubular integrity, packed clear cells, exfoliated cells, and round spermatids were profoundly noticed in the cauda epididymis. hyperthyroidism elevated the serum testosterone levels in H and DH mice and produced critical damages to the histoarchitecture of the epididymis. Collectively, this experiment endeavored to mimic the polyglandular autoimmune syndrome, which will be helpful to better understand the reasons for male infertility in diabetic-cum-hyperthyroid patients.


方法:32只ICR品系小鼠分为四组:对照组(C)、糖尿病组(D)、糖尿病+甲亢组(DH)和甲亢组(H)。D组小鼠以200 mg/kg剂量单次腹膜内注射链脲佐菌素(STZ),诱导糖尿病成功。另对其中一半以0.3 mg/kg剂量每天注射甲状腺素,组成DH组。小鼠试验结束后,采集睾丸、附睾和血液,并离心分离获得血清。睾丸和附睾用4%(0.04 g/ml)多聚甲醛固定,并用苏木精-伊红染色法(H & E)观察睾丸和附睾组织形态学变化,用放射免疫测定(RIA)试剂盒检测血清中睾酮、促甲状腺激素(TSH)、胰岛素、甲状腺素(T4)和三碘甲状腺原氨酸(T3)的含量并进行分析。
结论:D和DH组小鼠的体重、睾丸和附睾的重量显著降低。相比于正常甲亢或糖尿病小鼠,DH组中血糖水平显著升高。甲状腺激素可能是通过改变糖尿病患者的血清血糖水平对血糖稳态产生瞬时影响。 组织形态学分析结果显示,在DH和H组小鼠睾丸中,输精管管腔增大,上皮厚度减少,睾丸生殖干细胞发生萎缩性变化。DH组小鼠的附睾头呈现主细胞压实、纤毛、脂质空泡化和炎症浸润现象。在附睾尾部观察到了小管完整性受损、透明细胞聚积和细胞脱落,并发现圆形精子。对于DH和H组,甲亢提高了小鼠血清睾酮水平,并损害了附睾的组织形态。总之,本试验模拟了多腺体自身免疫综合征对雄性繁殖的影响,这将有助于更好地了解男性并发糖尿病和甲亢患者不育的原因。


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