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Journal of Zhejiang University SCIENCE B 1998 Vol.-1 No.-1 P.

http://doi.org/10.1631/jzus.B2200136


The role of melatonin receptor 1B gene polymorphisms and its effect on the regulation of glucose transport in gestational diabetes mellitus


Author(s):  Lijie WEI, Yi JIANG, Peng GAO, Jingyi ZHANG, Xuan ZHOU, Shenglan ZHU, Yuting CHEN, Huiting ZHANG, Yuanyuan DU, Chenyun FANG, Jiaqi LI, Xuan GAO, Mengzhou HE, Shaoshuai WANG, Ling FENG, Jun YU

Affiliation(s):  Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; more

Corresponding email(s):   yjtj2010@163.com

Key Words:  Gestational diabetes mellitus (GDM), Melatonin receptor 1B (MTNR1B), Single nucleotide polymorphism (SNP), Glucose uptake, Glucose transporters (GLUTs), Peroxisome proliferator-activated receptor gamma (PPARγ, )


Lijie WEI, Yi JIANG, Peng GAO, Jingyi ZHANG, Xuan ZHOU, Shenglan ZHU, Yuting CHEN, Huiting ZHANG, Yuanyuan DU, Chenyun FANG, Jiaqi LI, Xuan GAO, Mengzhou HE, Shaoshuai WANG, Ling FENG, Jun YU. The role of melatonin receptor 1B gene polymorphisms and its effect on the regulation of glucose transport in gestational diabetes mellitus[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .

@article{title="The role of melatonin receptor 1B gene polymorphisms and its effect on the regulation of glucose transport in gestational diabetes mellitus",
author="Lijie WEI, Yi JIANG, Peng GAO, Jingyi ZHANG, Xuan ZHOU, Shenglan ZHU, Yuting CHEN, Huiting ZHANG, Yuanyuan DU, Chenyun FANG, Jiaqi LI, Xuan GAO, Mengzhou HE, Shaoshuai WANG, Ling FENG, Jun YU",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200136"
}

%0 Journal Article
%T The role of melatonin receptor 1B gene polymorphisms and its effect on the regulation of glucose transport in gestational diabetes mellitus
%A Lijie WEI
%A Yi JIANG
%A Peng GAO
%A Jingyi ZHANG
%A Xuan ZHOU
%A Shenglan ZHU
%A Yuting CHEN
%A Huiting ZHANG
%A Yuanyuan DU
%A Chenyun FANG
%A Jiaqi LI
%A Xuan GAO
%A Mengzhou HE
%A Shaoshuai WANG
%A Ling FENG
%A Jun YU
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200136

TY - JOUR
T1 - The role of melatonin receptor 1B gene polymorphisms and its effect on the regulation of glucose transport in gestational diabetes mellitus
A1 - Lijie WEI
A1 - Yi JIANG
A1 - Peng GAO
A1 - Jingyi ZHANG
A1 - Xuan ZHOU
A1 - Shenglan ZHU
A1 - Yuting CHEN
A1 - Huiting ZHANG
A1 - Yuanyuan DU
A1 - Chenyun FANG
A1 - Jiaqi LI
A1 - Xuan GAO
A1 - Mengzhou HE
A1 - Shaoshuai WANG
A1 - Ling FENG
A1 - Jun YU
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200136


Abstract: 
Melatonin receptor 1B (MT2, encoded by the MTNR1B gene), a high-affinity receptor for melatonin, is associated with glucose homeostasis including glucose uptake and transport. rs10830963 variant in the MTNR1B gene is associated with glucose metabolic disorders including )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>gestational diabetes mellitus (GDM). However, the relationship between MT2-mediated melatonin signaling and high birth weight of GDM infant from maternal glucose abnormality remains unclear. This article aims to investigate the relationship between rs10830963 variants and GDM development, and the effects of MT2 receptor on glucose uptake and transport in trophoblasts. TaqMan-MGB probe quantitative polymerase chain reaction (quantitative PCR, qPCR) assays were used for rs10930963 genotyping. MT2 expression in the placenta of GDM and normal pregnant women was detected by immunofluorescence, western blot and real-time PCR. The relationship between MT2 and )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>glucose transporters (GLUTs) or PPARγ was detected by western blot, and glucose consumption of trophoblasts was detected by glucose assay kit. Results showed that the genotype and allele frequencies of rs10830963 were significantly different between GDM and normal pregnant women (P<0.05). Fasting, 1-hour, and 2-hour plasma glucose levels of G allele carriers were significantly higher than that of C allele carriers (P<0.05). The protein and mRNA expression of MT2 in the placenta of GDM were significantly higher than that of normal pregnant women (P<0.05). Melatonin could stimulate glucose uptake and GLUT4 and PPARγ protein expression in trophoblasts, which could be attenuated by MT2 receptor knockdown. In conclusion, rs10830963 variant was associated with an increased risk of GDM. MT2 receptor is essential for melatonin in raising glucose uptake and transport, which may be mediated by PPARγ.

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