Full Text:   <2008>

Summary:  <1776>

CLC number: R735.2

On-line Access: 2017-01-03

Received: 2016-05-10

Revision Accepted: 2016-08-01

Crosschecked: 2016-12-13

Cited: 1

Clicked: 3824

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zhong-dong Wang

http://orcid.org/0000-0002-9344-4748

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.1 P.27-36

http://doi.org/10.1631/jzus.B1600205


Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells


Author(s):  Zhong-dong Wang, Fan-yong Qu, Yuan-yuan Chen, Zhang-shen Ran, Hai-yan Liu, Hai-dong Zhang

Affiliation(s):  Clinical Laboratory of Taishan Sanatorium of Shandong Province, Tai’an 271001, China; more

Corresponding email(s):   zdongwang@yeah.net

Key Words:  miR-718, MicroRNA, Early growth response protein 3 (EGR3), Hepatocellular carcinoma (HCC), Malignant phenotype


Zhong-dong Wang, Fan-yong Qu, Yuan-yuan Chen, Zhang-shen Ran, Hai-yan Liu, Hai-dong Zhang. Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells[J]. Journal of Zhejiang University Science B, 2017, 18(1): 27-36.

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author="Zhong-dong Wang, Fan-yong Qu, Yuan-yuan Chen, Zhang-shen Ran, Hai-yan Liu, Hai-dong Zhang",
journal="Journal of Zhejiang University Science B",
volume="18",
number="1",
pages="27-36",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600205"
}

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%T Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells
%A Zhong-dong Wang
%A Fan-yong Qu
%A Yuan-yuan Chen
%A Zhang-shen Ran
%A Hai-yan Liu
%A Hai-dong Zhang
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600205

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A1 - Yuan-yuan Chen
A1 - Zhang-shen Ran
A1 - Hai-yan Liu
A1 - Hai-dong Zhang
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1600205


Abstract: 
Objective: hepatocellular carcinoma (HCC) is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. microRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 (miR-718) in the malignant phenotype of HCC cells and its possible role in HCC diagnosis. Methods: Here we first used a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, Transwell migration and invasion assays, and colony formation assay to evaluate the impact of miR-718 on the malignant phenotypes of HCC cells. Then, we used bioinformatic methods to predict the target gene of miR-718 and used green fluorescence protein (GFP) reporter assay, Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR) to validate the regulation relationship. Finally, we determined the role of the target gene in the HCC phenotype. Results: We found that the expression of miR-718 was significantly reduced in various HCC cell lines and HCC tissues. Re-expression of miR-718 significantly reduced the cellular viability and colony formation ability as well as inhibited the migration and invasion abilities of HCC cell lines. early growth response protein 3 (EGR3) is a direct target of miR-718 and is negatively regulated by miR-718. EGR3 could increase the viability and proliferation of HCC cells, and promot the migration and invasion of HCC cells. Conclusions: miR-718 acts as a tumor suppressive microRNA in HCC via regulating the expression of EGR3, which may provide a new diagnostic marker and treatment target for HCC.

miR-718和EGR3参与调控肝癌细胞系的恶性表型的研究

目的:研究miR-718和早期生长反应蛋白3(EGR3)在肝癌细胞系HepG2及SMMC-7721中对细胞恶性行为的作用。
创新点:首次在肝癌细胞系HepG2和SMMC-7721中发现miR-718作为抑癌基因及EGR3作为促癌基因起到调控肿瘤恶性行为的作用。
方法:采用聚合酶链反应(PCR)方法构建miR-718和EGR3的过表达及敲降质粒,并采用实时定量PCR(qRT-PCR)方法验证质粒有效性;采用MTT法和克隆形成实验检测肝癌细胞系HepG2和SMMC-7721的生长增殖能力;采用Transwell迁移侵袭实验检测肝癌细胞系HepG2和SMMC-7721的迁移和侵袭能力;采用增强型绿色荧光蛋白(EGFP)荧光报告载体实验验证miR-718的靶基因;采用qRT-PCR和蛋白质印迹(Western blot)检测相关基因表达水平的变化。
结论:miR-718在肝癌细胞系HepG2和SMMC-7721中起到抑癌基因的作用;EGR3在肝癌细胞系 HepG2和SMMC-7721中起到促癌基因的作用;miR-718是通过靶定EGR3 mRNA 3’ UTR下调EGR3的表达起到抑癌基因的作用。

关键词:miR-718;小非编码RNA;早期生长反应蛋白3;肝癌;表型

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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