CLC number: R735.7
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2020-02-22
Cited: 0
Clicked: 3929
Xiao-Long Wu, Ying Chen, Wen-Cui Kong, Zhong-Quan Zhao. Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway[J]. Journal of Zhejiang University Science B, 2020, 21(3): 234-245.
@article{title="Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway",
author="Xiao-Long Wu, Ying Chen, Wen-Cui Kong, Zhong-Quan Zhao",
journal="Journal of Zhejiang University Science B",
volume="21",
number="3",
pages="234-245",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900413"
}
%0 Journal Article
%T Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway
%A Xiao-Long Wu
%A Ying Chen
%A Wen-Cui Kong
%A Zhong-Quan Zhao
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 3
%P 234-245
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900413
TY - JOUR
T1 - Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway
A1 - Xiao-Long Wu
A1 - Ying Chen
A1 - Wen-Cui Kong
A1 - Zhong-Quan Zhao
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 3
SP - 234
EP - 245
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900413
Abstract: hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality globally. It accounts for the majority of primary liver cancer cases. amyloid precursor protein (APP), a cell membrane protein, plays a vital role in the pathogenesis of Alzheimer’s disease, and has been found to be implicated in tumor growth and metastasis. Therefore, to understand the relationship between APP and 5-fluorouracil (5-FU) resistance in liver cancer, Cell Counting Kit-8, apoptosis and cell cycle assays, western blotting, and reverse transcription-quantitative polymerase chain reaction (qPCR) analysis were performed. The results demonstrated that APP expression in Bel7402-5-FU cells was significantly up-regulated, as compared with that in Bel7402 cells. Through successful construction of APP-silenced (siAPP) and overexpressed (OE) Bel7402 cell lines, data revealed that the Bel7402-APP751-OE cell line was insensitive, while the Bel7402-siAPP cell line was sensitive to 5-FU in comparison to the matched control group. Furthermore, APP overexpression decreased, while APP silencing increased 5-FU-induced apoptosis in Bel7402 cells. Mechanistically, APP overexpression and silencing can regulate the mitochondrial apoptotic pathway and the expression of apoptotic suppressor genes (B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-xl)). Taken together, these results preliminarily revealed that APP overexpression contributes to the resistance of liver cancer cells to 5-FU, providing a new perspective for drug resistance.
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