CLC number: R96
On-line Access: 2017-06-05
Received: 2016-08-23
Revision Accepted: 2016-12-04
Crosschecked: 2017-05-10
Cited: 0
Clicked: 4385
Lan-lan Wan, Da-qi Zhang, Jin-nan Zhang, Li-qun Ren. Anti-hepatocarcinoma activity of TT-1, an analog of melittin, combined with interferon-α via promoting the interaction of NKG2D and MICA[J]. Journal of Zhejiang University Science B, 2017, 18(6): 522-531.
@article{title="Anti-hepatocarcinoma activity of TT-1, an analog of melittin, combined with interferon-α via promoting the interaction of NKG2D and MICA",
author="Lan-lan Wan, Da-qi Zhang, Jin-nan Zhang, Li-qun Ren",
journal="Journal of Zhejiang University Science B",
volume="18",
number="6",
pages="522-531",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600369"
}
%0 Journal Article
%T Anti-hepatocarcinoma activity of TT-1, an analog of melittin, combined with interferon-α via promoting the interaction of NKG2D and MICA
%A Lan-lan Wan
%A Da-qi Zhang
%A Jin-nan Zhang
%A Li-qun Ren
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 6
%P 522-531
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600369
TY - JOUR
T1 - Anti-hepatocarcinoma activity of TT-1, an analog of melittin, combined with interferon-α via promoting the interaction of NKG2D and MICA
A1 - Lan-lan Wan
A1 - Da-qi Zhang
A1 - Jin-nan Zhang
A1 - Li-qun Ren
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 6
SP - 522
EP - 531
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600369
Abstract: hepatocarcinoma is one of the malignant cancers with significant morbidity and mortality. immunotherapy has emerged in clinical treatment, owing to the limitation and severe side effects of chemotherapy. In the immune system, natural killer (NK) cells are important effectors required to eliminate malignant tumor cells without the limitation of major histocompatibility complex (MHC) molecule issues. Hence, treatment which could stimulate NK cells is of great interest. Here, we investigated the efficacy of the combined therapy of TT-1 (a mutant of melittin) and interferon-α; (IFN-α;) on NK cells and human liver cancer HepG-2/Huh7 cells in vitro and in vivo, as well as the mechanism involved. The combination therapy significantly inhibited the growth of HepG-2/Huh7 cells in vivo, but this effect was impaired after depleting NK cells. TT-1 not only up-regulated MHC class I-related chain molecules A (MICA) expression, but also prevented the secretion of soluble MICA (sMICA). Both the mRNA and protein of a disintegrin and metallopeptidase 10 (ADAM 10) in HepG-2/Huh7 cells were decreased after TT-1 treatment. The combined therapy of TT-1 and IFN-α could suppress the growth of HepG-2/Huh7 xenografted tumor effectively via promoting the interaction of NK group 2, member D (NKG2D) and MICA, indicating that TT-1+IFN-α would be a potential approach in treating liver cancer.
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