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On-line Access: 2017-10-05

Received: 2017-02-21

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Ling Hong


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.10 P.833-844


Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Author(s):  Ling Hong, Jun-li Liu, Samira Z. Midoun, Philip C. Miller

Affiliation(s):  Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; more

Corresponding email(s):   lhong@mail.hust.edu.cn, pcmiller@eng.ucsd.edu

Key Words:  Dunaliella salina, Transcriptome profile, Metabolic processes and adjustment, Regulatory metabolism, Salt stress

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Ling Hong, Jun-li Liu, Samira Z. Midoun, Philip C. Miller. Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina[J]. Journal of Zhejiang University Science B, 2017, 18(10): 833-844.

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author="Ling Hong, Jun-li Liu, Samira Z. Midoun, Philip C. Miller",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina
%A Ling Hong
%A Jun-li Liu
%A Samira Z. Midoun
%A Philip C. Miller
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700088

T1 - Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina
A1 - Ling Hong
A1 - Jun-li Liu
A1 - Samira Z. Midoun
A1 - Philip C. Miller
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 10
SP - 833
EP - 844
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700088

The unicellular green alga Dunaliella salina is well adapted to salt stress and contains compounds (including β-carotene and vitamins) with potential commercial value. A large transcriptome database of D. salina during the adjustment, exponential and stationary growth phases was generated using a high throughput sequencing platform. We characterized the metabolic processes in D. salina with a focus on valuable metabolites, with the aim of manipulating D. salina to achieve greater economic value in large-scale production through a bioengineering strategy. Gene expression profiles under salt stress verified using quantitative polymerase chain reaction (qPCR) implied that salt can regulate the expression of key genes. This study generated a substantial fraction of D. salina transcriptional sequences for the entire growth cycle, providing a basis for the discovery of novel genes. This first full-scale transcriptome study of D. salina establishes a foundation for further comparative genomic studies.


结论:通过杜氏盐藻转录组测序共获取了39 820条单一序列。在功能注释和聚类分析的基础上预测了杜氏盐藻盐胁迫下累积的渗透平衡产物(甘油和脯氨酸)、多胺以及类胡萝卜素的代谢路径。相关代谢途径的关键酶的表达谱分析,说明盐能够调节甘油、脯氨酸以及多胺的代谢过程。抑制精胺合成底物的供应可能会缓解盐藻增殖对胡萝卜素含量的影响。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[45]List of electronic supplementary materials

[46]Data S1 Sequences of the genes identified in D. salina transcriptome

[47]Table S1 Primers of those selective genes involved in the metabolic processes in D. salina

[48]Table S2 Summary of annotation of D. salina transcriptome

[49]Table S3 Top-hit species (viridiplantae) list of D. salina BLAST-annotated uniseqs

[50]Table S4 Enzymes identified in metabolism of osmolytes (glycerol and proline), polyamines, and carotenoid through annotation of D. salina transcriptome

[51]Table S5 The best hit of the highlighted enzymes in the metabolic processes of D. salina

[52]Fig. S1 KOG (euKaryotic Ortholog Groups) functional classification of D. salina uniseqs

[53]Fig. S2 Gene ontology (GO) annotation of D. salina transcriptome

[54]Fig. S3 KEGG functional analyses of D. salina uniseqs

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