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CLC number: S432.1

On-line Access: 2019-01-07

Received: 2017-12-09

Revision Accepted: 2018-06-17

Crosschecked: 2018-12-05

Cited: 0

Clicked: 2696

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Cai-ge Lu

https://orcid.org/0000-0002-6862-3488

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.1 P.84-94

http://doi.org/10.1631/jzus.B1700609


Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola


Author(s):  Mi Ni, Qiong Wu, Hong-li Wang, Wei-cheng Liu, Bin Hu, Dian-peng Zhang, Juan Zhao, De-wen Liu, Cai-ge Lu

Affiliation(s):  Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; more

Corresponding email(s):   liuwich@163.com, lcgf88@sina.com

Key Words:  Streptomyces blastmyceticus, Peach brown rot, Monilinia fructicola, Biocontrol efficacy


Mi Ni, Qiong Wu, Hong-li Wang, Wei-cheng Liu, Bin Hu, Dian-peng Zhang, Juan Zhao, De-wen Liu, Cai-ge Lu. Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola[J]. Journal of Zhejiang University Science B, 2019, 20(1): 84-94.

@article{title="Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola",
author="Mi Ni, Qiong Wu, Hong-li Wang, Wei-cheng Liu, Bin Hu, Dian-peng Zhang, Juan Zhao, De-wen Liu, Cai-ge Lu",
journal="Journal of Zhejiang University Science B",
volume="20",
number="1",
pages="84-94",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700609"
}

%0 Journal Article
%T Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola
%A Mi Ni
%A Qiong Wu
%A Hong-li Wang
%A Wei-cheng Liu
%A Bin Hu
%A Dian-peng Zhang
%A Juan Zhao
%A De-wen Liu
%A Cai-ge Lu
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 1
%P 84-94
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700609

TY - JOUR
T1 - Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola
A1 - Mi Ni
A1 - Qiong Wu
A1 - Hong-li Wang
A1 - Wei-cheng Liu
A1 - Bin Hu
A1 - Dian-peng Zhang
A1 - Juan Zhao
A1 - De-wen Liu
A1 - Cai-ge Lu
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 1
SP - 84
EP - 94
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700609


Abstract: 
peach brown rot, caused by Monilinia fructicola, is one of the most serious peach diseases. A strain belonging to the Actinomycetales, named Streptomyces blastmyceticus JZB130180, was found to have a strong inhibitory effect on M. fructicola in confrontation culture. Following the inoculation of peaches in vitro, it was revealed that the fermentation broth of S. blastmyceticus JZB130180 had a significant inhibitory effect on disease development by M. fructicola. The fermentation broth of S. blastmyceticus JZB130180 had an EC50 (concentration for 50% of maximal effect) of 38.3 µg/mL against M. fructicola, as determined in an indoor toxicity test. Analysis of the physicochemical properties of the fermentation broth revealed that it was tolerant of acid and alkaline conditions, temperature, and ultraviolet radiation. In addition, chitinase, cellulase, and protease were also found to be secreted by the strain. The results of this study suggest that S. blastmyceticus JZB130180 may be used for the biocontrol of peach brown rot.

一株新型稻瘟霉素链霉菌JZB130180的鉴定及其对桃褐腐病的生防效果评价

目的:筛选对桃褐腐病有显著防效的链霉菌并解析其生防因子.
创新点:本研究筛选到一株新型稻瘟霉素链霉菌JZB130180,其对桃褐腐病具有显著防效.通过对其生防因子解析及发酵液分析,为后续分离抑菌次生代谢物奠定了基础.
方法:通过对峙培养和离体实验,检测链霉菌对桃褐腐病的抑制作用.通过平皿抑菌实验,计算链霉菌发酵液的室内毒力;随后进行了发酵液的理化性质检测.通过酶活力测定等,解析链霉菌中的生防因子.
结论:链霉菌JZB130180及其发酵液对桃褐腐病具有显著的抑制作用.发酵液对桃褐腐病菌的半数有效抑制浓度(EC50)为38.3 µg/mL,且对酸碱、温度和紫外线都有一定的耐受能力.此外,该菌株可以分泌几丁质酶、纤维素酶、蛋白酶等重要生防因子.

关键词:稻瘟霉素链霉菌;桃褐腐病;褐腐病菌;生防效果

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[37]List of electronic supplementary materials

[38]Table S1 Primers used in this study

[39]Table S2 Solubility determination of active substances

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