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CLC number: Q943.2

On-line Access: 2019-04-01

Received: 2018-01-03

Revision Accepted: 2018-06-19

Crosschecked: 2019-03-01

Cited: 0

Clicked: 2940

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hong-wu Bian

https://orcid.org/0000-0001-9786-5964

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.4 P.322-331

http://doi.org/10.1631/jzus.B1800003


Regulation of flowering time via miR172-mediated APETALA2-like expression in ornamental gloxinia (Sinningia speciosa)


Author(s):  Xiao-yan Li, Fu Guo, Sheng-yun Ma, Mu-yuan Zhu, Wei-huai Pan, Hong-wu Bian

Affiliation(s):  Institute of Genetic and Regenerative Biology, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   whpan@usx.edu.cn, hwbian@zju.edu.cn

Key Words:  Flowering time, Transgenic gloxinia, MicroRNA172, APETALA2-like (AP2-like)


Xiao-yan Li, Fu Guo, Sheng-yun Ma, Mu-yuan Zhu, Wei-huai Pan, Hong-wu Bian. Regulation of flowering time via miR172-mediated APETALA2-like expression in ornamental gloxinia (Sinningia speciosa)[J]. Journal of Zhejiang University Science B, 2019, 20(4): 322-331.

@article{title="Regulation of flowering time via miR172-mediated APETALA2-like expression in ornamental gloxinia (Sinningia speciosa)",
author="Xiao-yan Li, Fu Guo, Sheng-yun Ma, Mu-yuan Zhu, Wei-huai Pan, Hong-wu Bian",
journal="Journal of Zhejiang University Science B",
volume="20",
number="4",
pages="322-331",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800003"
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%0 Journal Article
%T Regulation of flowering time via miR172-mediated APETALA2-like expression in ornamental gloxinia (Sinningia speciosa)
%A Xiao-yan Li
%A Fu Guo
%A Sheng-yun Ma
%A Mu-yuan Zhu
%A Wei-huai Pan
%A Hong-wu Bian
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 4
%P 322-331
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800003

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T1 - Regulation of flowering time via miR172-mediated APETALA2-like expression in ornamental gloxinia (Sinningia speciosa)
A1 - Xiao-yan Li
A1 - Fu Guo
A1 - Sheng-yun Ma
A1 - Mu-yuan Zhu
A1 - Wei-huai Pan
A1 - Hong-wu Bian
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 4
SP - 322
EP - 331
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800003


Abstract: 
We investigated the microRNA172 (miR172)-mediated regulatory network for the perception of changes in external and endogenous signals to identify a universally applicable floral regulation system in ornamental plants, manipulation of which could be economically beneficial. transgenic gloxinia plants, in which miR172 was either overexpressed or suppressed, were generated using Agrobacterium-mediated transformation. They were used to study the effect of altering the expression of this miRNA on time of flowering and to identify its mRNA target. Early or late flowering was observed in transgenic plants in which miR172 was overexpressed or suppressed, respectively. A full-length complementary DNA (cDNA) of gloxinia (Sinningia speciosa) APETALA2-like (SsAP2-like) was identified as a target of miR172. The altered expression levels of miR172 caused up- or down-regulation of SsAP2-like during flower development, which affected the time of flowering. Quantitative real-time reverse transcription PCR analysis of different gloxinia tissues revealed that the accumulation of SsAP2-like was negatively correlated with the expression of miR172a, whereas the expression pattern of miR172a was negatively correlated with that of miR156a. Our results suggest that transgenic manipulation of miR172 could be used as a universal strategy for regulating time of flowering in ornamental plants.

MiR172介导的AP2-like转录因子表达对大岩桐花期调控的研究

目的:本文通过研究microRNA172a(miR172a)对成花途径中关键靶基因APETALA2类(AP2-like)的调控作用及其机理分析,探索通过操纵miRNA表达改变观赏植物花期的基因工程分子育种新策略.
创新点:本研究基于植物miR172序列和功能的高度保守性,通过转基因的方法操纵大岩桐miR172的表达,进而影响AP2-like基因的表达,并起到调控花期的作用.
方法:本研究借用拟南芥miR172a的已知序列构建组成型过表达载体35S:miR172a和抑制miR172表达的35S:MIM172载体.利用农杆菌介导法成功获得了35S:miR172a过表达株系以及抑制miR172作用的35S:MIM172株系,并利用cDNA末端快速扩增技术(rapid amplification of cDNA ends,RACE)克隆得到了大岩桐AP2-like cDNA全序列,并通过实时荧光定量聚合酶链式反应技术(qPCR)检测转基因株系中AP2-like的表达变化.
结论:短日照条件下,35S:miR172a过表达株系花期比野生型提前(47.00±2.16)天;35S:MIM172株系花期延迟(7.00±4.28)天.在35S:miR172a过表达株系中miR172的表达水平明显上升,其靶基因SsAP2-like的表达量明显下降;35S:MIM172株系中miR172的积累水平受到抑制,而SsAP2-like的表达量明显上升,表明miR172介导调控SsAP2的表达对大岩桐成花转变具有促进作用.通过改变miR172的表达调控关键靶基因进而改变花期的方法可以作为调节观赏植物开花时间的有效策略.

关键词:花期;大岩桐;MicroRNA172;APETALA2-like(AP2-like)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[28]List of electronic supplementary materials

[29]Table S1 Culture medium used in this study

[30]Table S2 Primers used in the experiments

[31]Fig. S1 PCR analysis of Pre-AtmiR172a and MIM172 (35S: MIM172) in transgenic gloxinias

[32]Data S1 Nucleotide sequence of SsAP2-like

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