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CLC number: R285.5

On-line Access: 2020-09-07

Received: 2020-05-27

Revision Accepted: 2020-07-16

Crosschecked: 2020-08-17

Cited: 0

Clicked: 2116

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Mee Lee Looi

https://orcid.org/0000-0001-5415-6973

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.9 P.745-748

http://doi.org/10.1631/jzus.B2000278


Anti-migratory effects of Piper betle leaf aqueous extract on cancer cells and its microtubule targeting properties


Author(s):  Mee Lee Looi, Alwyn Khai Howe Wong, Shelly Anne Gnapragasan, Anis Zafirah Japri, Aiysvariyah Rajedadram, Kar Yong Pin

Affiliation(s):  Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; more

Corresponding email(s):   meelee.looi@um.edu.my

Key Words:  Piper betle, Cell migration, Microtubules, Tubulin network


Mee Lee Looi, Alwyn Khai Howe Wong, Shelly Anne Gnapragasan, Anis Zafirah Japri, Aiysvariyah Rajedadram, Kar Yong Pin. Anti-migratory effects of Piper betle leaf aqueous extract on cancer cells and its microtubule targeting properties[J]. Journal of Zhejiang University Science B, 2020, 21(9): 745-748.

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author="Mee Lee Looi, Alwyn Khai Howe Wong, Shelly Anne Gnapragasan, Anis Zafirah Japri, Aiysvariyah Rajedadram, Kar Yong Pin",
journal="Journal of Zhejiang University Science B",
volume="21",
number="9",
pages="745-748",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000278"
}

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%T Anti-migratory effects of Piper betle leaf aqueous extract on cancer cells and its microtubule targeting properties
%A Mee Lee Looi
%A Alwyn Khai Howe Wong
%A Shelly Anne Gnapragasan
%A Anis Zafirah Japri
%A Aiysvariyah Rajedadram
%A Kar Yong Pin
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
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A1 - Mee Lee Looi
A1 - Alwyn Khai Howe Wong
A1 - Shelly Anne Gnapragasan
A1 - Anis Zafirah Japri
A1 - Aiysvariyah Rajedadram
A1 - Kar Yong Pin
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DOI - 10.1631/jzus.B2000278


Abstract: 
Piper betle (PB), also known as “betel” in Malay language, is a tropical Asian vine. PB leaves are commonly chewed by Asians along with betel quid. It contains phenols such as eugenol and hydroxychavicol along with chlorophyll, β-carotene, and vitamin C (Salehi et al., 2019). Extracts from PB leaves have various medicinal properties including anticancer, antioxidant, anti-inflammatory, and antibacterial effects (Salehi et al., 2019). Previous research has shown that PB induces cell cycle arrest at late S or G2/M phase and causes apoptosis at higher doses (Wu et al., 2014; Guha Majumdar and Subramanian, 2019). A combination of PB leaf extract has also been shown to enhance the cytotoxicity of the anticancer drug, 5-fluorouracil (5-FU), in cancer cells (Ng et al., 2014).

槟榔叶水提液对癌细胞的抗迁移作用及其微管靶向性的研究

概要:本文研究了槟榔(Piper betle,PB)水提取物对癌细胞转移能力的调节作用及其对微管蛋白结构和网络的影响.采用亚毒剂量对PB和5-氟尿嘧啶(5-FU)处理的癌细胞进行抗迁移研究,观察细胞迁移20小时.在所有处理组中,100 µg/mL PB处理的癌细胞均显示出最大的抗迁移作用(P=0.016).总体上,PB对癌细胞的抗迁移作用高于5-FU.通过细胞形态观察发现,PB处理的细胞表现出与标准微管抑制剂(紫杉醇)相似的细胞特征,并在PB和紫杉醇处理的癌细胞中发现M期细胞群.通过对微管结构和网络进一步研究,我们发现PB和紫杉醇处理的癌细胞表现出长期破坏的纺锤体.因此,我们认为PB具有对癌细胞的抗迁移作用,并可能改变蛋白结构和网络.
关键词:槟榔;细胞迁移;微管;微管蛋白网络

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Belotti D, Vergani V, Drudis T, et al., 1996. The microtubule-affecting drug paclitaxel has antiangiogenic activity. Clin Cancer Res, 2(11):1843-1849.

[2]Blajeski AL, Phan VA, Kottke TJ, et al., 2002. G1 and G2 cell-cycle arrest following microtubule depolymerization in human breast cancer cells. J Clin Invest, 110(1):91-99.

[3]Cadart C, Zlotek-Zlotkiewicz E, le Berre M, et al., 2014. Exploring the function of cell shape and size during mitosis. Dev Cell, 29(2):159-169.

[4]Gandalovičová A, Rosel D, Fernandes M, et al., 2017. Migrastatics—anti-metastatic and anti-invasion drugs: promises and challenges. Trends Cancer, 3(6):391-406.

[5]Ganguly A, Yang HL, Sharma R, et al., 2012. The role of microtubules and their dynamics in cell migration. J Biol Chem, 287(52):43359-43369.

[6]Ganguly A, Cabral F, Yang HL, et al., 2015. Peloruside A is a microtubule-stabilizing agent with exceptional anti-migratory properties in human endothelial cells. Oncoscience, 2(6):585-595.

[7]https://doi.org/10.18632/oncoscience.169

[8]Guha Majumdar A, Subramanian M, 2019. Hydroxychavicol from Piper betle induces apoptosis, cell cycle arrest, and inhibits epithelial-mesenchymal transition in pancreatic cancer cells. Biochem Pharmacol, 166:274-291.

[9]Hayot C, Debeir O, van Ham P, et al., 2006. Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicol Appl Pharmacol, 211(1):30-40.

[10]Mukhtar E, Adhami VM, Mukhtar H, 2014. Targeting microtubules by natural agents for cancer therapy. Mol Cancer Ther, 13(2):275-284.

[11]Ng PL, Rajab NF, Then SM, et al., 2014. Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 15(8):692-700.

[12]Perez EA, 2009. Microtubule inhibitors: differentiating tubulin-inhibiting agents based on mechanisms of action, clinical activity, and resistance. Mol Cancer Ther, 8(8):2086-2095.

[13]Salehi B, Zakaria ZA, Gyawali R, et al., 2019. Piper species: a comprehensive review on their phytochemistry, biological activities and applications. Molecules, 24(7):1364.

[14]Shakibaei M, Kraehe P, Popper B, et al., 2015. Curcumin potentiates antitumor activity of 5-fluorouracil in a 3D alginate tumor microenvironment of colorectal cancer. BMC Cancer, 15:250.

[15]Théry M, Bornens M, 2008. Get round and stiff for mitosis. HFSP J, 2(2):65-71.

[16]Wu PF, Tseng HC, Chyau CC, et al., 2014. Piper betle leaf extracts induced human hepatocellular carcinoma HEP3B cell death via MAPKS regulating the p73 pathway in vitro and in vivo. Food Funct, 5(12):3320-3328.

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