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On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2021-01-06
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Aiysvariyah RAJEDADRAM, Kar Yong PIN, Sui Kiong LING, See Wan YAN, Mee Lee LOOI. Hydroxychavicol, a polyphenol from Piper betle leaf extract, induces cell cycle arrest and apoptosis in TP53-resistant HT-29 colon cancer cells[J]. Journal of Zhejiang University Science B, 2021, 22(2): 112-122.
@article{title="Hydroxychavicol, a polyphenol from Piper betle leaf extract, induces cell cycle arrest and apoptosis in TP53-resistant HT-29 colon cancer cells",
author="Aiysvariyah RAJEDADRAM, Kar Yong PIN, Sui Kiong LING, See Wan YAN, Mee Lee LOOI",
journal="Journal of Zhejiang University Science B",
volume="22",
number="2",
pages="112-122",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000446"
}
%0 Journal Article
%T Hydroxychavicol, a polyphenol from Piper betle leaf extract, induces cell cycle arrest and apoptosis in TP53-resistant HT-29 colon cancer cells
%A Aiysvariyah RAJEDADRAM
%A Kar Yong PIN
%A Sui Kiong LING
%A See Wan YAN
%A Mee Lee LOOI
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 2
%P 112-122
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000446
TY - JOUR
T1 - Hydroxychavicol, a polyphenol from Piper betle leaf extract, induces cell cycle arrest and apoptosis in TP53-resistant HT-29 colon cancer cells
A1 - Aiysvariyah RAJEDADRAM
A1 - Kar Yong PIN
A1 - Sui Kiong LING
A1 - See Wan YAN
A1 - Mee Lee LOOI
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 2
SP - 112
EP - 122
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000446
Abstract: This study aims to elucidate the antiproliferative mechanism of hydroxychavicol (HC). Its effects on cell cycle, apoptosis, and the expression of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) in HT-29 colon cancer cells were investigated. HC was isolated from Piper betle leaf (PBL) and verified by high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of the standard drug 5-fluorouracil (5-FU), PBL water extract, and HC on HT-29 cells were measured after 24, 48, and 72 h of treatment. cell cycle and apoptosis modulation by 5-FU and HC treatments were investigated up to 30 h. Changes in phosphorylated JNK (pJNK) and P38 (pP38) MAPK expression were observed up to 18 h. The half maximal inhibitory concentration (IC50) values of HC (30 ?g/mL) and PBL water extract (380 ?g/mL) were achieved at 24 h, whereas the IC50 of 5-FU (50 ?mol/L) was obtained at 72 h. cell cycle arrest at the G0/G1 phase in HC-treated cells was observed from 12 h onwards. Higher apoptotic cell death in HC-treated cells compared to 5-FU-treated cells (P<0.05) was observed. High expression of pJNK and pP38 MAPK was observed at 12 h in HC-treated cells, but not in 5-FU-treated HT-29 cells (P<0.05). It is concluded that HC induces cell cycle arrest and apoptosis of HT-29 cells, with these actions possibly mediated by JNK and P38 MAPK.
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