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CLC number: R965

On-line Access: 2016-09-07

Received: 2016-01-16

Revision Accepted: 2016-04-10

Crosschecked: 2016-08-18

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Ye Zhang


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.9 P.692-702


Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide

Author(s):  Yun-zhi Ling, Xiao-hong Li, Li Yu, Ye Zhang, Qi-sheng Liang, Xiao-di Yang, Hong-tao Wang

Affiliation(s):  Department of Anesthesiology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China; more

Corresponding email(s):   yuli95@163.com, zhang_ye011@163.com

Key Words:  Parecoxib, Primary astrocyte, Hydrogen peroxide (H2O2), Brain-derived neurotrophic factor (BDNF), Bax, Bcl-2

Yun-zhi Ling, Xiao-hong Li, Li Yu, Ye Zhang, Qi-sheng Liang, Xiao-di Yang, Hong-tao Wang. Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide[J]. Journal of Zhejiang University Science B, 2016, 17(9): 692-702.

@article{title="Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide",
author="Yun-zhi Ling, Xiao-hong Li, Li Yu, Ye Zhang, Qi-sheng Liang, Xiao-di Yang, Hong-tao Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide
%A Yun-zhi Ling
%A Xiao-hong Li
%A Li Yu
%A Ye Zhang
%A Qi-sheng Liang
%A Xiao-di Yang
%A Hong-tao Wang
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 9
%P 692-702
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600017

T1 - Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide
A1 - Yun-zhi Ling
A1 - Xiao-hong Li
A1 - Li Yu
A1 - Ye Zhang
A1 - Qi-sheng Liang
A1 - Xiao-di Yang
A1 - Hong-tao Wang
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 9
SP - 692
EP - 702
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600017

Objective: To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H2O2) in rat astrocytes in vitro. Methods: All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H2O2 treatment group, 100 μmol/L H2O2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 μmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 μmol/L H2O2. Several indices were investigated, and the expressions of bax, bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified. Results: Compared to the NC group, exposure to H2O2 resulted in significant morphological changes, which could be reversed by pretreatment of parecoxib. In addition, H2O2 treatment led to loss of viability (P=0.026) and increased intracellular reactive oxygen species (ROS) levels (P<0.001), and induced apoptosis (P<0.01) in the primary astrocytes relative to the NC group. However, in the parecoxib pretreatment groups, all the above changes reversed significantly (P<0.05) as compared to the H2O2 treatment group, and were nearly unchanged when compared to the NC group. Mechanical investigation showed that dysregulated bax, bcl-2, and BDNF could be implicated in these changes. Conclusions: Our results indicated that parecoxib provided a protective effect from oxidative stress induced by exposure to H2O2.


方法:设立如下四组对照:(1)阴性对照组;(2)100 µmol/L H2O2处理组(处理时间为24 h);(3)80 µmol/L 帕瑞昔布处理组;(4)160 µmol/L帕瑞昔布处理组(第三和第四组先用帕瑞昔布处理24 h,再用100 µmol/L H2O2处理24 h)。用荧光显微镜观察星形胶质细胞的形态,用MTT法检测星形胶质细胞的存活率,用荧光探针二氯荧光黄双乙酸盐(DCDHF-DA)检测星形胶质细胞内氧自由基的含量,并用碘化丙啶(PI)染色检测细胞的凋亡状态。最后用反转录酶聚合酶链反应(RT-PCR)和蛋白质印迹(Western blot)检测Bax、Bcl-2和BDNF三种蛋白在4组中的表达水平。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[24]List of electronic supplementary materials

[25]Fig. S1 Identification of primary astrocytes using GFAP staining

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