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On-line Access: 2021-02-07

Received: 2020-08-10

Revision Accepted: 2020-10-08

Crosschecked: 2021-01-06

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Huixia SHOU


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.2 P.99-111


Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing

Author(s):  Qi WANG, Xiaoxia SHEN, Tian QIU, Wei WU, Lin LI, Zhi'an WANG, Huixia SHOU

Affiliation(s):  State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   huixia@zju.edu.cn

Key Words:  DNA extraction, Cellulose filter paper, Field test, Genetically modified (GM) crops, Chinese medicine ultra-fine powder, Rapid molecular identification

Qi WANG, Xiaoxia SHEN, Tian QIU, Wei WU, Lin LI, Zhi'an WANG, Huixia SHOU. Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing[J]. Journal of Zhejiang University Science B, 2021, 22(2): 99-111.

@article{title="Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing",
author="Qi WANG, Xiaoxia SHEN, Tian QIU, Wei WU, Lin LI, Zhi'an WANG, Huixia SHOU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing
%A Xiaoxia SHEN
%A Tian QIU
%A Wei WU
%A Lin LI
%A Zhi'an WANG
%A Huixia SHOU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 2
%P 99-111
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000465

T1 - Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing
A1 - Qi WANG
A1 - Xiaoxia SHEN
A1 - Tian QIU
A1 - Wei WU
A1 - Lin LI
A1 - Zhi'an WANG
A1 - Huixia SHOU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 2
SP - 99
EP - 111
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000465

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification. In this study, a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin (PVC) sheet. This modified method is named EZ-D, for EASY DNA extraction. Compared with the original cetyl trimethylammonium bromide (CTAB) method, DNA extracted by EZ-D is more efficient in polymerase chain reaction (PCR) amplification due to the more stable performance of the EZ-D stick. The EZ-D method is also faster, easier, and cheaper. PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples. A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL. Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80% in GC content. EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues. Moreover, when EZ-D was combined with the loop-mediated isothermal amplification (LAMP) method, DNA identification of biological samples could be achieved without the need for specialized equipment. As an optimized DNA purification method, EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.


目的:本研究旨在开发一种优化的滤纸法快速纯化DNA技术--EZ-D(EASY DNA),并将EZ-D与聚合酶链式反应(PCR)/环介导等温扩增(LAMP)技术相结合应用于实验室或室外不同类型植物材料的目的基因的快速鉴定,同时对EZ-D方法的DNA提取效率和所提取DNA的完整性和可扩增能力进行评价。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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