CLC number:
On-line Access: 2022-04-22
Received: 2020-10-10
Revision Accepted: 2021-01-13
Crosschecked: 2022-04-22
Cited: 0
Clicked: 3965
Citations: Bibtex RefMan EndNote GB/T7714
https://orcid.org/0000-0003-3035-278X
Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU. Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances[J]. Journal of Zhejiang University Science B, 2021, 22(7): 533-547.
@article{title="Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances",
author="Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU",
journal="Journal of Zhejiang University Science B",
volume="22",
number="7",
pages="533-547",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000602"
}
%0 Journal Article
%T Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances
%A Zhijing LIU
%A Cong XU
%A Ran TIAN
%A Wan WANG
%A Jiage MA
%A Liya GU
%A Fei LIU
%A Zhanmei JIANG
%A Juncai HOU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 7
%P 533-547
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000602
TY - JOUR
T1 - Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances
A1 - Zhijing LIU
A1 - Cong XU
A1 - Ran TIAN
A1 - Wan WANG
A1 - Jiage MA
A1 - Liya GU
A1 - Fei LIU
A1 - Zhanmei JIANG
A1 - Juncai HOU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 7
SP - 533
EP - 547
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000602
Abstract: Lactic acid bacteria (LAB) are a representative probiotic. As the dominant flora in the human intestinal tract, LAB can regulate the balance of human intestinal flora and improve host health. The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population, with excellent probiotics and high antibacterial activity. After 16S ribosomal RNA (rRNA) homology and phylogenetic tree analysis, potential probiotics were tested for their antibacterial activity, resistance to artificial gastrointestinal fluid and drugs, surface hydrophobicity, and safety. Three strains of LAB with acid resistance, bile salt resistance, epithelial cell adhesion, and no multidrug resistance were selected: Lactobacillus salivarius, Leuconostoc lactis, and Lactobacillus paracasei. Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L. lactis were organic acids, whereas those of L. salivarius and L. paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity. These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens.
[1]AlshammariE, PatelM, SachidanandanM, et al., 2019. Potential evaluation and health fostering intrinsic traits of novel probiotic strain Enterococcus durans F3 isolated from the gut of fresh water fish Catla catla. Food Sci Anim Resour, 39(5):844-861.
[2]AmorimJC, PiccoliRH, DuarteWF, 2018. Probiotic potential of yeasts isolated from pineapple and their use in the elaboration of potentially functional fermented beverages. Food Res Int, 107:518-527.
[3]AymesF, MonnetC, CorrieuG, 1999. Effect of α-acetolactate decarboxylase inactivation onα-acetolactate and diacetyl production by Lactococcus lactis subsp. lactis biovar diacetylactis. J Biosci Bioeng, 87(1):87-92.
[4]BansalR, JainA, GoyalM, et al., 2019. Antibiotic abuse during endodontic treatment: a contributing factor to antibiotic resistance. J Family Med Prim Care, 8(11):3518-3524.
[5]BegleyM, GahanCGM, HillC, 2005. The interaction between bacteria and bile. FEMS Microbiol Rev, 29(4):625-651.
[6]CárdenasN, MartínV, ArroyoR, et al., 2019. Prevention of recurrent acute otitis media in children through the use of Lactobacillus salivarius PS7, a target-specific probiotic strain. Nutrients, 11(2):376.
[7]ChavagnatF, HaueterM, JimenoJ, et al., 2002. Comparison of partial tuf gene sequences for the identification of lactobacilli. FEMS Microbiol Lett, 217(2):177-183.
[8]CocolinL, ManzanoM, CantoniC, et al., 2001. Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages. Appl Environ Microbiol, 67(11):5113-5121.
[9]CotterPD, HillC, 2003. Surviving the acid test: responses of Gram-positive bacteria to low pH. Microbiol Mol Biol Rev, 67(3):429-453.
[10]FečkaninováA, KoščováJ, MudroňováD, et al., 2019. Characterization of two novel lactic acid bacteria isolated from the intestine of rainbow trout (Oncorhynchus mykiss, Walbaum) in Slovakia. Aquaculture, 506:294-301.
[11]GadGFM, Abdel-HamidAM, FaragZSH, 2014. Antibiotic resistance in lactic acid bacteria isolated from some pharmaceutical and dairy products. Braz J Microbiol, 45(1):25-33.
[12]GarsaAK, KumariyaR, SoodSK, et al., 2014. Bacteriocin production and different strategies for their recovery and purification. Probiotics Antimicrob Proteins, 6(1):47-58.
[13]GiraffaG, ChanishviliN, WidyastutiY, 2010. Importance of lactobacilli in food and feed biotechnology. Res Microbiol, 161(6):480-487.
[14]HammamiI, RhoumaA, JaouadiB, et al., 2009. Optimization and biochemical characterization of a bacteriocin from a newly isolated Bacillus subtilis strain 14B for biocontrol of Agrobacterium spp. strains. Lett Appl Microbiol, 48(2):253-260.
[15]HolsP, Ledesma-GarcíaL, GabantP, et al., 2019. Mobilization of microbiota commensals and their bacteriocins for therapeutics. Trends Microbiol, 27(8):690-702.
[16]HuJ, MaLB, NieYF, et al., 2018. A microbiota-derived bacteriocin targets the host to confer diarrhea resistance in early-weaned piglets. Cell Host Microbe, 24(6):817-832.e8.
[17]JalilpourY, AbdollahzadeB, ParviziFardG, et al., 2017. A simple route for preparation of pH-sensitive hydrogels by using egg white proteins in alginate scaffold for the encapsulation of probiotics. Ars Pharm, 58(3):127-136.
[18]JubairWK, HendricksonJD, SeversEL, et al., 2018. Modulation of inflammatory arthritis in mice by gut microbiota through mucosal inflammation and autoantibody generation. Arthritis Rheumatol, 70(8):1220-1233.
[19]KhannaS, ToshPK, 2014. A clinician’s primer on the role of the microbiome in human health and disease. Mayo Clin Proc, 89(1):107-114.
[20]KılıçGB, KarahanAG, 2010. Identification of lactic acid bacteria isolated from the fecal samples of healthy humans and patients with dyspepsia, and determination of their pH, bile, and antibiotic tolerance properties. J Mol Microbiol Biotechnol, 18(4):220-229.
[21]KimSG, BecattiniS, MoodyTU, et al., 2019. Microbiota-derived lantibiotic restores resistance against vancomycin-resistant Enterococcus. Nature, 572(7771):665-669.
[22]KordelM, SchüllerF, SahlHG, 1989. Interaction of the pore forming-peptide antibiotics Pep 5, nisin and subtilin with non-energized liposomes. FEBS Lett, 244(1):99-102.
[23]Krzych-FałtaE, FurmańczykK, TomaszewskaA, et al., 2018. Probiotics: myths or facts about their role in allergy prevention. Adv Clin Exp Med, 27(1):119-124.
[24]LähteinenT, MalinenE, KoortJMK, et al., 2010. Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces. Anaerobe, 16(3):293-300.
[25]LappeR, Sant'AnnaV, BrandelliA, 2012. Extraction of the antimicrobial peptide cerein 8A by aqueous two-phase systems and aqueous two-phase micellar systems. Nat Prod Res, 26(23):2259-2265.
[26]le BarzM, DanielN, VarinTV, et al., 2019. In vivo screening of multiple bacterial strains identifies Lactobacillus rhamnosus Lb102 and Bifidobacterium animalis ssp. lactis Bf141 as probiotics that improve metabolic disorders in a mouse model of obesity. FASEB J, 33(4):4921-4935.
[27]LiongMT, ShahNP, 2005. Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. J Dairy Sci, 88(1):55-66.
[28]LiuWJ, BaoQH, Jirimutu, et al., 2012. Isolation and identification of lactic acid bacteria from Tarag in Eastern Inner Mongolia of China by 16S rRNA sequences and DGGE analysis. Microbiol Res, 167(2):110-115.
[29]MarkoD, 2009. Food-borne mycotoxins. Mol Nutr Food Res, 53(4):421-421.
[30]MilioniC, MartínezB, Degl'InnocentiS, et al., 2015. A novel bacteriocin produced by Lactobacillus plantarum LpU4 as a valuable candidate for biopreservation in artisanal raw milk cheese. Dairy Sci Technol, 95(4):479-494.
[31]NuobarieneL, CizeikieneD, GradzeviciuteE, et al., 2015. Phytase-active lactic acid bacteria from sourdoughs: isolation and identification. LWT-Food Sci Technol, 63(1):766-772.
[32]OteroMC, Nader-MacíasME, 2006. Inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus gasseri isolated from the vaginal tract of cattle. Anim Reprod Sci, 96(2):35-46.
[33]ÖzogulF, HamedI, 2018. The importance of lactic acid bacteria for the prevention of bacterial growth and their biogenic amines formation: a review. Crit Rev Food Sci Nutr, 58(10):1660-1670.
[34]PeiJJ, LiXS, HanH, et al., 2018. Purification and characterization of plantaricin SLG1, a novel bacteriocin produced by Lb. plantarum isolated from yak cheese. Food Control, 84:111-117.
[35]ReubenRC, RoyPC, SarkarSL, et al., 2020. Characterization and evaluation of lactic acid bacteria from indigenous raw milk for potential probiotic properties. J Dairy Sci, 103(2):1223-1237.
[36]RolhionN, ChassaingB, NahoriMA, et al., 2019. A Listeria monocytogenes bacteriocin can target the commensal prevotella copri and modulate intestinal infection. Cell Host Microbe, 26(5):691-701.
[37]Sant'AnnaV, Folmer CorreaAP, de Souza da Motta A, et al., 2016. Liquid-liquid extraction of antimicrobial peptide P34 by aqueous two-phase and micellar systems. Prep Biochem Biotechnol, 46(8):838-843.
[38]ShafeiHA, SabourHA, IbrahimN, et al., 2000. Isolation, screening and characterization of bacteriocin-producing lactic acid bacteria isolated from traditional fermented food. Microbiol Res, 154(4):321-331.
[39]ShahandashtiRV, KermanshahiRK, GhadamP, 2016. The inhibitory effect of bacteriocin produced by Lactobacillus acidophilus ATCC 4356 and Lactobacillus plantarum ATCC 8014 on planktonic cells and biofilms of Serratia marcescens. Turk J Med Sci, 46(4):1188-1196.
[40]SimhaBV, SoodSK, KumariyaR, et al., 2012. Simple and rapid purification of pediocin PA-1 from Pediococcus pentosaceous NCDC 273 suitable for industrial application. Microbiol Res, 167(9):544-549.
[41]SvetochEA, EruslanovBV, LevchukVP, et al., 2011. Isolation of Lactobacillus salivarius 1077 (NRRL B-50053) and characterization of its bacteriocin, including the antimicrobial activity spectrum. Appl Environ Microbiol, 77(8):2749-2754.
[42]TuomolaE, CrittendenR, PlayneM, et al., 2001. Quality assurance criteria for probiotic bacteria. Am J Clin Nutr, 73(2):393s-398s.
[43]Usman, HosonoA, 1999. Bile tolerance, taurocholate deconjugation, and binding of cholesterol by Lactobacillus gasseri strains. J Dairy Sci, 82(2):243-248.
[44]YerlikayaO, 2019. Probiotic potential and biochemical and technological properties of Lactococcus lactis ssp. lactis strains isolated from raw milk and kefir grains. J Dairy Sci, 102(1):124-134.
[45]ZhaiQX, ShenXD, CenS, et al., 2020. Screening of Lactobacillus salivarius strains from the feces of Chinese populations and the evaluation of their effects against intestinal inflammation in mice. Food Funct, 11(1):221-235.
[46]ZhangM, HangXM, FanXB, et al., 2008. Characterization and selection of Lactobacillus strains for their effect on bile tolerance, taurocholate deconjugation and cholesterol removal. World J Microbiol Biotechnol, 24(1):7-14.
[47]ZhengG, SlavikMF, 1999. Isolation, partial purification and characterization of a bacteriocin produced by a newly isolated Bacillus subtilis strain. Lett Appl Microbiol, 28(5):363-367.
Open peer comments: Debate/Discuss/Question/Opinion
<1>