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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zhijing LIU

https://orcid.org/0000-0003-3035-278X

Zhanmei JIANG

https://orcid.org/0000-0002-7296-658X

Juncai HOU

https://orcid.org/0000-0003-2943-3398

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.7 P.533-547

http://doi.org/10.1631/jzus.B2000602


Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances


Author(s):  Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU

Affiliation(s):  Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China; more

Corresponding email(s):   zhanmeijiang@neau.edu.cn, jchou@neau.edu.cn

Key Words:  Intestinal lactic acid bacteria, Screening, Probiotic, Bacteriostatic substance


Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU. Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances[J]. Journal of Zhejiang University Science B, 2021, 22(7): 533-547.

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author="Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU",
journal="Journal of Zhejiang University Science B",
volume="22",
number="7",
pages="533-547",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000602"
}

%0 Journal Article
%T Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances
%A Zhijing LIU
%A Cong XU
%A Ran TIAN
%A Wan WANG
%A Jiage MA
%A Liya GU
%A Fei LIU
%A Zhanmei JIANG
%A Juncai HOU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 7
%P 533-547
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000602

TY - JOUR
T1 - Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances
A1 - Zhijing LIU
A1 - Cong XU
A1 - Ran TIAN
A1 - Wan WANG
A1 - Jiage MA
A1 - Liya GU
A1 - Fei LIU
A1 - Zhanmei JIANG
A1 - Juncai HOU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 7
SP - 533
EP - 547
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000602


Abstract: 
Lactic acid bacteria (LAB) are a representative probiotic. As the dominant flora in the human intestinal tract, LAB can regulate the balance of human intestinal flora and improve host health. The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population, with excellent probiotics and high antibacterial activity. After 16S ribosomal RNA (rRNA) homology and phylogenetic tree analysis, potential probiotics were tested for their antibacterial activity, resistance to artificial gastrointestinal fluid and drugs, surface hydrophobicity, and safety. Three strains of LAB with acid resistance, bile salt resistance, epithelial cell adhesion, and no multidrug resistance were selected: Lactobacillus salivarius, Leuconostoc lactis, and Lactobacillus paracasei. Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L. lactis were organic acids, whereas those of L. salivarius and L. paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity. These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens.

肠源益生性抑菌乳酸菌的筛选及其抑菌物质的研究

目的:筛选出适合中国人群肠道特点、具有优良益生性和高抑菌活性的乳酸菌,探究其抑菌活性物质。
创新点:从中国健康成人粪便中分离出了具有良好潜在益生性和安全性的乳酸菌,发现了其对大肠杆菌和金黄色葡萄球菌有较高的抑菌活性。同时探究出了乳酸明串珠菌的主要抑菌物质为有机酸,唾液乳杆菌和副干酪乳杆菌的主要抑菌物质为有机酸和细菌素,而且该细菌素具有广谱的抗菌活性。
方法:通过分离纯化、菌株形态学鉴定、16SrRNA同源性和系统发育树分析初步鉴定乳酸菌;采用牛津杯双层琼脂扩散法筛选出抑菌乳酸菌;通过人工模拟胃肠液和药敏试纸法评价乳酸菌的潜在益生性和耐药性;通过不同酶、pH和热处理对益生性乳酸菌的抑菌物质及性质进行分析;采用双水相萃取法对抑菌物质进行纯化;最后通过Tricine-SDS-PAGE电泳对抑菌物质分子量进行预测及抑菌普测定。
结论:从中国健康成人的粪便中分离筛选出3株具有良好潜在益生性和安全性的乳酸菌菌株,对大肠杆菌ATCC 25922和金黄色葡萄球菌ATCC 25923均有较好的抑菌活性。乳酸明串珠菌的主要抑菌物质为有机酸,唾液乳杆菌和副干酪乳杆菌的主要抑菌物质为有机酸和广谱抑菌活性的细菌素。

关键词:肠道乳酸菌;筛选;益生性;抑菌物质

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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