CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2021-02-23
Cited: 0
Clicked: 3566
Weifeng CHEN, Zhiwei LI, Xingxing DONG, Xiaohong WANG. A tetravalent single-chain variable fragment antibody for the detection of staphylococcal enterotoxin A[J]. Journal of Zhejiang University Science B, 2021, 22(4): 305-309.
@article{title="A tetravalent single-chain variable fragment antibody for the detection of staphylococcal enterotoxin A",
author="Weifeng CHEN, Zhiwei LI, Xingxing DONG, Xiaohong WANG",
journal="Journal of Zhejiang University Science B",
volume="22",
number="4",
pages="305-309",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000661"
}
%0 Journal Article
%T A tetravalent single-chain variable fragment antibody for the detection of staphylococcal enterotoxin A
%A Weifeng CHEN
%A Zhiwei LI
%A Xingxing DONG
%A Xiaohong WANG
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 4
%P 305-309
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000661
TY - JOUR
T1 - A tetravalent single-chain variable fragment antibody for the detection of staphylococcal enterotoxin A
A1 - Weifeng CHEN
A1 - Zhiwei LI
A1 - Xingxing DONG
A1 - Xiaohong WANG
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 4
SP - 305
EP - 309
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000661
Abstract: Staphylococcal enterotoxin A (SEA) synthesized by Staphylococcus aureus is a foodborne and heat-stable toxin, which is a great threat to human health (
[1]ChenWF, HuL, LiuAP, et al., 2014. Expression and characterization of single-chain variable fragment antibody against staphylococcal enterotoxin A in Escherichia coli. Can J Microbiol, 60(11):737-743.
[2]DasguptaA, 2019. Biotin and Other Interferences in Immunoassays: A Concise Guide. Elsevier, St. Louis.
[3]LiangB, ZhangYX, LiuAP, et al., 2011. Production of a monoclonal antibody by simultaneous immunization of staphylococcal enterotoxin A and B. Appl Biochem Biotechnol, 164(6):831-840.
[4]LiuJL, AndersonGP, GoldmanER, 2007. Isolation of anti-toxin single domain antibodies from a semi-synthetic spiny dogfish shark display library. BMC Biotechnol, 7:78.
[5]MiyanabeK, AkibaH, KurodaD, et al., 2018. Intramolecular H-bonds govern the recognition of a flexible peptide by an antibody. J Biochem, 164(1):65-76.
[6]MuraseT, EugenioL, SchorrM, et al., 2014. Structural basis for antibody recognition in the receptor-binding domains of toxins A and B from Clostridium difficile. J Biol Chem, 289(4):2331-2343.
[7]NouriA, AhariH, ShahbazzadehD, 2018. Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotoxin A in raw milk samples. Int J Biol Macromol, 107:1732-1737.
[8]O'KennedyR, 2019. Rapid Antibody-based Technologies in Food Analysis. The United Kingdom by CPI Group (UK) Ltd., Croydon, UK.
[9]OrtegaE, AbriouelH, LucasR, et al., 2010. Multiple roles of Staphylococcus aureus enterotoxins: pathogenicity, superantigenic activity, and correlation to antibiotic resistance. Toxins (Basel), 2(8):2117-2131.
[10]PexaraA, BourrielA, GovarisA, 2010. Staphylococcus aureus and staphylococcal enterotoxins in foodborne diseases. J Hellenic Vet Med Soc, 61(4):316-322.
[11]PinchukIV, BeswickEJ, ReyesVE, 2010. Staphylococcal enterotoxins. Toxins (Basel), 2(8):2177-2197.
[12]PolonelliL, PontonJ, ElguezabalN, et al., 2008. Antibody complementarity-determining regions (CDRs) can display differential antimicrobial, antiviral and antitumor activities. PLoS ONE, 3(6):e2371.
[13]ThomasR, PatenaudeSI, MacKenzieCR, et al., 2002. Structure of an anti-blood group A Fv and improvement of its binding affinity without loss of specificity. J Biol Chem, 277(3):2059-2064.
Open peer comments: Debate/Discuss/Question/Opinion
<1>